Conserved Protein Domain Family
PX_SNX19_like_plant

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cd06872: PX_SNX19_like_plant 
The phosphoinositide binding Phox Homology domain of uncharacterized SNX19-like plant proteins
The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to PI-enriched membranes. Members in this subfamily are uncharacterized plant proteins containing an N-terminal PXA domain, a central PX domain, and a C-terminal domain that is conserved in some sorting nexins (SNXs). This is the same domain architecture found in SNX19. SNX13 and SNX14 also contain these three domains but also contain a regulator of G protein signaling (RGS) domain in between the PXA and PX domains. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction.
Statistics
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PSSM-Id: 132782
View PSSM: cd06872
Aligned: 4 rows
Threshold Bit Score: 175.787
Threshold Setting Gi: 116059283
Created: 10-Jul-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              ###                        ##             #
gi 168023479  826 LHCRVLVAHFEKigsrTFAVYIIQVKDtenrTWQIQRRFRNFEQLHRRLKDMPYY---NLTLPPKRFLSSSldsTFVRER 902
gi 5306243    528 LKCRVLGAYFEKqgskSFAVYSIAVTDvenkTWFVKRRYSNFERLHRQLKEIPNY---NLQLPPKRIFSSStedAFVHRR 604
gi 116059283  330 LSARVTGAEIVGsgssSYAVYLVTVTTneneMWVVPRRFRNFETLHRRLREVDKVavnALEFPSKSWIKLSlsgAFIESR 409
gi 5103823    613 LRCEVLGANIVKgsskMFAVYSVAVTDesnhSWSIKRRFRHFEELHRRLKVFPEY---KLHLPPKHFLSTGvdiPVIQER 689
                          90       100       110
                  ....*....|....*....|....*....|..
Feature 1                                         
gi 168023479  903 CTLLDKYLKDLLAIp--SVAELHEVWDFLSLN 932
gi 5306243    605 CIQLDKYLQDLLCIa--NVAEQHEVWDFLSAA 634
gi 116059283  410 WKALDAYLRAVLVNe--KLAASAEVFSFMDAR 439
gi 5103823    690 CVLLDEYIKLQRISgsiEVWDFLSVDSQTYAF 721

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