Conserved Protein Domain Family
PX_SNX14

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cd06877: PX_SNX14 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 14
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX14 may be involved in recruiting other proteins to the membrane via protein-protein and protein-ligand interaction. It is expressed in the embryonic nervous system of mice, and is co-expressed in the motoneurons and the anterior pituary with Islet-1. SNX14 shows a similar domain architecture as SNX13, containing an N-terminal PXA domain, a regulator of G protein signaling (RGS) domain, a PX domain, and a C-terminal domain that is conserved in some SNXs.
Statistics
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PSSM-Id: 132787
View PSSM: cd06877
Aligned: 13 rows
Threshold Bit Score: 152.145
Threshold Setting Gi: 110759141
Created: 10-Jul-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                         ###            
gi 86129486  554 SAWKISIPYVDFfddpsl----erkdreERIPVFCIDVErnd--------rraVGHEPEHWSVYRRYLEFYVLESKLTEF 621
gi 119569018 458 AAWKISIPYVDFfedpss----erkekkERIPVFCIDVErnd--------rraVGHEPEHWSVYRRYLEFYVLESKLTEF 525
gi 113679934 555 SAWTISIPYVDFydde---------vkkERIPVFCIDVErnd--------rknVGHETESWSVYRKYVEFYVLESKLTEF 617
gi 147901161 564 SAWKISIPYVDFfedtnf----ekkdrkERIPVFCIDVErnd--------rkaVGHEPEHWSVYRKYIEFYVLESKLTEF 631
gi 189235075 548 SSWRVSILSYQTn---------------NKVVSFCINVQrld---------vvEDVTKRHWIVLRKDQDFYTLKAKLVEF 603
gi 170576188 589 SKWNVTIPCVEPrrdp---------vggHTMYVYVISVErfdlnddidqtslsATVAPQKWSLIRHYNEFYILESKLVEF 659
gi 71998307  591 NCWKVNVAQVNGlrdk---------isdRVIFVFIIEVErp----------daKPHETQRWSIHRSFNEFYVLESKLNEF 651
gi 193699981 502 STWKVTDVTSAMkles--------gsngGQSLVFFITVEt------------vKNDLIKQWCVERRLADFYTLKSKLTEF 561
gi 219467363 509 SAWRVAIPRAESrstdvvl--gfryengKQYYVYIISVQrid--------ireGGKLFSHFPLYRRYNEFYVLENKLTEF 578
gi 198435340 556 STWRIVIPRVDVlrrddeisrgssastnEKMYAFCVEVKrvd--------iheDDDMPIQWQVLRRYNEFYVLENKLTEF 627
gi 110759141 528 TTWRISIPHIETg---------------DNQTLYVIAVH--------------NIAEGKSWTVLRRDQDFYVLRTRLAEF 578
gi 115717872 207 SAWRVSIPGVGMkme-----------ggKEGFVFIIDIQrvd--------vreGDGERNSWRVERMYHEFYALEQKLREF 267
gi 215509167 514 SAWRVSIPDVEMrvd----------mqhRHYLAFIVEVQridv-------gdgDSPDDLHWTVARRYHEFYALESKLLEF 576
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
Feature 1                     ##             #                              
gi 86129486  622 HGtf--pDAQLPSKRiigp-knyEFLKSKREEFQEYLQKLLQHpELSNSQLLADFLSPN 677
gi 119569018 526 HGaf--pDAQLPSKRiigp-knyEFLKSKREEFQEYLQKLLQHpELSNSQLLADFLSPN 581
gi 113679934 618 HGpf--qDAQLPSKRiigp-knyEFLSSKRGEFEEYLQKLLHHpELSNSQLLADFLSPF 673
gi 147901161 632 HGtf--pDAQLPSKRiigp-knyEFLKSKREEFQEYLQNLLQHpELSNSQLLADFLSPN 687
gi 189235075 604 HGeneisDTPLPSRKa------gSSIEVRMKKYEEFLTKLLQKpLLRGSDLLFSFLTAE 656
gi 170576188 660 HGnt-ikTESLPPRRffnc-xsrVYVESRREIFERFIQLLAKQrALKQSDLLFVFLSTE 716
gi 71998307  652 HGds-lrFSPLPTRKtfvt-rdkPFMEQHRLIFSTFLSTLSKQrILNRSELLLAFLSSN 708
gi 193699981 562 HGvf--vDARLPSRRi------lLQKTENNDIYMQFLNQLLQKpELRGSDLLHMFLTSI 612
gi 219467363 579 HGetlleDCCLPTKRiqltkpsqEFIDSKIAIFEKWLQVLLTKpALRGSELVHSFLSPG 637
gi 198435340 628 HGsl--aPASLPPKKfmr--rspEFLDSKKADFERFLKLLIGKpGLQHSELLYDFLLPE 682
gi 110759141 579 HGdkelsDSPLPSRKnp-----hSSCAINRQRYEDFLEKLLSKpVLRSSELLYNFLTVP 632
gi 115717872 268 HGvf--eDAQLPVRRpfgs-ksqDFMEKKRPGLEKYLRVLLTKpILRGSQLLYTFLTTN 323
gi 215509167 577 HGel--pVSPLPPRRsp----srQQQPPCRNALEKYLRDLLTVpRLRSSELLYSFLRHD 629

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