Conserved Protein Domain Family
PX_SNX25

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cd06878: PX_SNX25 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 25
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. The function of SNX25 is not yet known. It has been found in exosomes from human malignant pleural effusions. SNX25 shows the same domain architecture as SNX13 and SNX14, containing an N-terminal PXA domain, a regulator of G protein signaling (RGS) domain, a PX domain, and a C-terminal domain that is conserved in some SNXs.
Statistics
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PSSM-Id: 132788
View PSSM: cd06878
Aligned: 12 rows
Threshold Bit Score: 172.944
Threshold Setting Gi: 115689651
Created: 10-Jul-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
putative
Feature 1:putative phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       ##
gi 118090087  503 WWCENLGMWKAFIISGEv-----lEENGEQVPCYSVLVSlqevg-----------------------gaetkNWTVPRKL 554
gi 47214113   571 WWCENLGHWRANITAAEa-----tEEGGEMVACYSVCVSlvege-----------------------estnsRWNVNRKL 622
gi 157117553  613 VWGEYLGKWRAIVESVDf------SDDKEPQPQFMIVVQvdevndcgkedv---------atamdaadsistGWVVVRSL 677
gi 156375362  497 RWCENIGNWNVQIEGVEw-----nNEDDKWIPLYVIVVHcspinlpispsg---------aspdrlpgatseGWVVTRKF 562
gi 190584545  620 LWCDLMGQWRTSISNAHv-----vVEGDGYVPVYVILVErassgredsn--------------dketrtisdCWAVTRTL 680
gi 219439392  469 QWWDMIGRWRANICNAEf-----iVNEEKIEPVFVIVVHisgaias-------------------tdestttGWVVMRNL 524
gi 19527695   645 AWTEHLGKWKATIQSVE-------VSDEKESLQFMILVHvdedinapvqptsskngdsghanlrkrpsgissGWVVMRSL 717
gi 198430697  574 AWWEHMGSWAANVQDAYcqqdsseGSLNGGGPLYTIVVQcsgvsly-------------------eatddvsGWVVTRCL 634
gi 115689651  561 RIEEKLSNKSQALANLKst----hGVDSKMEQLLEIEIEklklqkkrvelyieq----tdlwtehidkiptsGWVVSRRL 632
gi 193787897  374 WWCENLGMWKASITSGEv-----tEENGEQLPCYFVMVSlqevg-----------------------gvetkNWTVPRRL 425
gi 110764995  593 MWAEHLGHWKAEVQSAE-------IPDNGESPQFVLVVHmrenedn--------------------nesiysGWVVLRKL 645
gi 212505448  568 VWEEHLGKWRVVVQSAE-------VSADKECPHFVLVVHvledeln--------------------ydaistGWVVLRKL 620
                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
Feature 1         #                          ##             #                              
gi 118090087  555 SEFQNLHRKLSECFPslkkvQLPSLSKLPFKSi--DQKFMEKSKNQLNNFLQKLLSDeRLCQSEALYAFLSPS 625
gi 47214113   623 PEFQMLHRKLTECFPslkklQLPSLSKLPFKSi--DQKFLDKSKTQLNAFLQRLLTDeRLCQSEALYAFLSPS 693
gi 157117553  678 AQFHDLHRKLRPMCSdlktiDLPSNNAFKLFLlknDKSLLEKSKQQIQKYLNFILEDdHLNQSEALYEFLSPS 750
gi 156375362  563 NDFKILHAKLKECSSkl-pnELPAPAKKWYKSl--DDEFLNKSRLALEQYLQSLLADeTLCLSEELYSFLSPS 632
gi 190584545  681 NEFQELHHTLTDCYPwl-rkNFPILSKKWTKSi--AQTTLDRIKGLLENYLQGLILHeKLCQSDVLYLFLCPS 750
gi 219439392  525 SDFHALHKRLKEYRSwl--rEKDLPSKRWFRAt--DKAFLDKARTMLEAYLNEVLADdTLCQSQVLFSFLSPS 593
gi 19527695   718 NQVHELQRKLRHVSSnlkaiDLPTNFKFFFLKt--DRHGQEKAKSQIQKFLNFILEDdHLNGSEAIYTFLSPS 788
gi 198430697  635 SDFHNLHHSLKEYSSwlrkqSLPKLPMFRMRGv--SEKFLLESKSTLNTYLHNILKDeILRCSQIVYSFLSPG 705
gi 115689651  633 GEFHDLQNRLKECSHwlakvKLPQNNKKLFNRe-tMNEFLERSKNELQKHLTAILGDaTLRHSEALYSFLIPK 704
gi 193787897  426 SEFQNLHRKLSECVPslkkvQLPSLSKLPFKSi--DQKFMEKSKNQLNKFLQNLLSDeRLCQSEALYAFLSPS 496
gi 110764995  646 QDFQDLHRKLRQLCSnvknlDLPSQPLKFFGKs--DKNTLDKAKIQIQKYLNFVLEDeKLNQSEALYTFLSPS 716
gi 212505448  621 SEFHELHKKLSQVWNgvknlELPSQSPKFLFGkhsDKASLDKAKNQIQKYLEFVLQDeRLNTSEAIYTFLSPS 693

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