1H6H,2DYB


Conserved Protein Domain Family
PX_p40phox

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cd06882: PX_p40phox 
Click on image for an interactive view with Cn3D
The phosphoinositide binding Phox Homology domain of the p40phox subunit of NADPH oxidase
The PX domain is a phosphoinositide binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. p40phox contains an N-terminal PX domain, a central SH3 domain that binds p47phox, and a C-terminal PB1 domain that interacts with p67phox. It is a cytosolic subunit of the phagocytic NADPH oxidase complex (also called Nox2 or gp91phox) which plays a crucial role in the cellular response to bacterial infection. NADPH oxidase catalyzes the transfer of electrons from NADPH to oxygen during phagocytosis forming superoxide and reactive oxygen species. p40phox positively regulates NADPH oxidase in both phosphatidylinositol-3-phosphate (PI3P)-dependent and PI3P-independent manner. The PX domain is a phospholipid-binding module involved in the membrane targeting of proteins. The p40phox PX domain binds to PI3P, an abundant lipid in phagosomal membranes, playing an important role in the localization of NADPH oxidase. The PX domain of p40phox is also involved in protein-protein interaction.
Statistics
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PSSM-Id: 132792
View PSSM: cd06882
Aligned: 7 rows
Threshold Bit Score: 186.872
Threshold Setting Gi: 195998215
Created: 14-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..PB1 domain
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Structure:1H6H_A; Human P40(phox) PX domain binds Phosphatidylinositol 3-Phosphate; defined at 3.5A contacts.
    View structure with Cn3D
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                ###                                ##   
1H6H_A        18 DVAISANIADIEEKRGF-TSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGpdsks-salaCTLPTLPAKVYVG 95
2DYB_A        21 DVAISANIADIEEKRGF-TSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGpdsks-salaXTLPTLPAKVYVG 98
gi 147904961  19 DVPVSAHIADMEERRGF-SFYYTFVIEVKTKGGSKYFIYRRYSQFFALQTKLEENYGpekgi-apyiCTLPELPPKIFVG 96
gi 86129452   19 DVPVSANIADIEEKKGF-TNYYMFVIEVKIKSGGRYLIFRRYREFYALHTKLEERYGgeskn-saftCTLPVLPGKVYVG 96
gi 94733412   19 NIPVTATIADIEEKKGF-IVYFSFVIEVKTKGNSKYLIYRRYREFFALHQSLELKYTaeaqs-gyynCQLPTLPGKVFMG 96
gi 219408304  17 NVAVTAKIVDLEKRRGI-TNYYVYVTEVTLNNGSSYKIFRRYSQFDALQSDLLARFPieagdidakdRVLPSLPGKIYFG 95
gi 195998215   5 ELAIDATILDCHKVQLEnTKYYVYSIKVILHSGETHVIYRRFRQFNGLLLHLERRFPleagkvnpqdRILPTVPGKIIFG 84
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
Feature 1                  #                                    
1H6H_A        96 VKQ--EIAEMRIPALNAYMKSLLSLpvWVLMDEDVRIFFYQSPYDSE 140
2DYB_A        99 VKQ--EIAEMRIPALNAYMKSLLSLpvWVLMDEDVRIFFYQSPYDSE 143
gi 147904961  97 NKK--DIAETRIPLLNGYMKRLLNSpvWLLLDEDLRLFYYQTLSDSE 141
gi 86129452   97 AKR--EIAENRIPILNIYMKNLLCLpvWVLMDEEVRLFFYHSNFDSE 141
gi 94733412   97 NKK--EIAESRIPELNSYMKRLLCLptWVLLDDLIRMFFYQTESDSQ 141
gi 219408304  96 RSAirEVAERRKPELNKYMQELLSLdpKISRDPDVRLFFGQTESDVL 142
gi 195998215  85 RSAkrEVAERRLPLLDRYVRELLSLhrKISQCDLIQSFLRQNKEDYD 131

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