2V6V,2CZO


Conserved Protein Domain Family
PX_Bem1p

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cd06890: PX_Bem1p 
Click on image for an interactive view with Cn3D
The phosphoinositide binding Phox Homology domain of Bem1p
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Members of this subfamily bear similarity to Saccharomyces cerevisiae Bem1p, containing two Src Homology 3 (SH3) domains at the N-terminus, a central PX domain, and a C-terminal PB1 domain. Bem1p is a scaffolding protein that is critical for proper Cdc42p activation during bud formation in yeast. During budding and mating, Bem1p migrates to the plasma membrane where it can serve as an adaptor for Cdc42p and some other proteins. Bem1p also functions as an effector of the G1 cyclin Cln3p and the cyclin-dependent kinase Cdc28p in promoting vacuolar fusion. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction. The PX domain of Bem1p specifically binds phosphatidylinositol-4-phosphate (PI4P).
Statistics
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PSSM-Id: 132800
View PSSM: cd06890
Aligned: 22 rows
Threshold Bit Score: 94.2777
Threshold Setting Gi: 68474314
Created: 11-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:The PX domain of Bem1p binds phosphatidylinositol-4-phosphate (PI4P).
  • Comment:based on the structures of phosphoinositide-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              ###                                       
2V6V_A        19 VKASVESFGLe-dEKYWFLVCCELsn----gKTRQLKRYYQDFYDLQVQLLDAFPAEAGklrda-----------ggqws 82
gi 45200784  134 CWCCVTCCGIr-eSRVWYRIECMLrs----gYQRTLCRYYHDFYWLHVGLIGRLQQLGLgv------------------d 190
gi 50308785  192 NDCKVVSVAIk-dNRMWYRVETTTss----qHKRFLCRYYQDFYQLHCQLLDEFRRLSLd-------------------- 246
gi 68474314  334 FEIDQIFYNKs-nKSFWYKIKYYDnl---nhQIIYLGKFYKDFYQLHDGIISQYNNNNNnnnnnnnanninnkntndlsi 409
gi 50427181  131 KSASISNVLLl-dNRYWYRLDITYsd----nSKTYVPRYYEDFYNLHGSLLNMVGKNIDtn-----------------gi 188
gi 150864035 168 VSACVAKVYSdstNKYWYRTRMTMst----nEDIYVGKCYQQFYKLHTALVSKYPEETLp-------------------- 223
gi 50289491  200 TKCEVESIELk-dNRLEYTVRIQDse----gNTKVKHIYYSAFYKLHTKLMAGLKENMSl-------------------- 254
gi 156847598 176 EDVSVQSCKLisvDIVEKRVQYTLnlrlannGENIVHRYYGQFYKLHTKLLSIGSNYNEi-------------------- 235
gi 190347966 134 VNCSISNCLSf-dNRYWYRLDVTYsd----hSQSYICRYYQDFYELHIALVQCKNSLAAtsis-------------dpke 195
gi 150864034 130 TSVSISNFLLs-eQRYWYRVDVVYsd----hSKAYLCRYYQDFYNLHIKLLGLMAASSQiv-----------------se 187
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1                ##                   #                                  
2V6V_A        83 KRIXPYIPGPVPyv--------tNSITKKRKEDLNIYVADLVNLp---dYISRSEXVHSLFVVL 135
gi 45200784  191 LNQLPKLPAPAGnk-------lkNSSAEIRMESFNKYLRELFESthipgHVRDSLVVDQWLAPR 247
gi 50308785  247 RKHLPNLPKPMAns-------ksNESVHSRLNSFTAYLEVLLSSehiptDFKQSIFYDQWLCPK 303
gi 68474314  410 KLPPPILRNSLYhspnvkfdknlIENSYKLLIRLNDYLNQLINHwp--nILNECPKFHDFIYNC 471
gi 50427181  189 SPSLPKLPEPIPptass-qskdlIVMLLKRCNDLSIYTNRLFNDk----RFQRSKALYEWLDVS 247
gi 150864035 224 KLPLPVRSSSVSgnkry-stlkyDRKFLKDLNALSEGLNSYFNNlllirSIQTSSEVYSFIFDS 286
gi 50289491  255 PNIPAPSSNYAQek-------vdESTKLDRLQMFNTYLTYLIDVi---yDIKSPLHLQDTFTNW 308
gi 156847598 236 KLPSLPHPLPFTgsl-----ehnSTLINDRVQEFNDYLANLLDNi----LNSSSIVLKDLINNW 290
gi 190347966 196 TIQLPKLPEPIPstcid-dkhqlMSLLLKRCNDLHVYINKLILNk----NYQTCETFMNWLDLD 254
gi 150864034 188 ELKLPKLPEPLPtnhdv-edssqITSLLKRCNALNIYVNKLILNk----YYQTSQVLLNWLDLN 246

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