Conserved Protein Domain Family
PX_Vps17p

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cd06891: PX_Vps17p 
The phosphoinositide binding Phox Homology domain of yeast sorting nexin Vps17p
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Vsp17p forms a dimer with Vps5p, the yeast counterpart of human SNX1, and is part of the retromer complex that mediates the transport of the carboxypeptidase Y receptor Vps10p from endosomes to Golgi. Similar to Vps5p and SNX1, Vps17p harbors a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. The PX-BAR structural unit helps determine specific membrane localization.
Statistics
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PSSM-Id: 132801
View PSSM: cd06891
Aligned: 14 rows
Threshold Bit Score: 165.988
Threshold Setting Gi: 71005760
Created: 10-Jul-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
putative
Feature 1:putative phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 2507154    85 QQPSQPSErvilp---------------ersdEKKKYTLLAKVTGLERfgsatgkkenPTIIFDCSTN-LPTFRKQQYKN 148
gi 50426315   60 EKLSEEEMrkll-----------------perFTDKYQINIKLTAIEKnka-----gnPILRFDATVKgLPKFRSETYKD 117
gi 159126347 100 QYTNEPRSqlpgrsqt---------garqnakNGPAYKIQAKITGLERtgk-----kdPILRFDVHTN-VPKFRTTQYRD 164
gi 50290269   59 TQPKQMESeetkkknaekqqeilkkliperfeQKDKYNVVIKVIGLERvgslsnkkenPTIIFEVNTN-LPTFRKKNHKN 137
gi 170090790  62 QIYGQPEAglisprel--------vgsngvgyEKPGPYLRVRITGLDRnr------rdILVKLDAQTN-LSNFTGTTYRN 126
gi 58259037   95 FQQSQQETpagev---------------qqerKAPRVYLRVRIGALERnk------kdLLIRFDASTN-LSTYKNGMYRN 152
gi 74625805   73 EATMNPSAassttg------------ensisqTGSGPFLRIRIVDIEAens-----kdPVIKMNVQTT-LPAYRSKLYKN 134
gi 68475097   51 EPSPDPNSesiltede--------lrkliperFTTKYSLSIQLVEIEKnkp-----gnPILKMNVKVKgLPRYRQSIYKD 117
gi 213402931 160 SQENEERStvsk----------------knatSEPKFMVSFRIASIEIvdk-----hdPILKMNVTTN-CPGYRSKHYRD 217
gi 156838632  50 ETDKAAEApkknv--------------lpersSNQKFNITVKVTGLERagstnnkkenPTIVLDLNTN-LPTFRKQTHKK 114
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
Feature 1           ###                      ##              #                              
gi 2507154   149 VKKSYEEFHQLFKYLNVAIQESFVPTLPSAYTtfGINSEEDRMKVTRNFQLWFNRLSQDpLIIRNEEVAFFIESD 223
gi 50426315  118 IRRTYNEIVKFNKYLTVSNLEVFVPVIPLAITsyPANGEDEAKQLHSIWQEWFNRITSNpILIRDEEFVYFIEND 192
gi 159126347 165 VRRTHAEFAKLADHLISANPEVLVPAVPPPLTpaGAGTEEDEIRVKASMQRWLNIVMSNeILMQDDEVVLFVESD 239
gi 50290269  138 VRKSLDEFRNLFKYLNATLSESFIPSLPAPFTnyGINTKEDYKKTVNNFQVWFFRLCANpLIIRNEELAFFIESD 212
gi 170090790 127 VSRSYLEFQQFYETLVQSNPQTIIPALPLAQTs-APTDEEDDRLVKIMLQRWITRVSEDsILIQDEDLRSFIESD 200
gi 58259037  153 MQRSYVEFQKFAEQLSLVCPQTIIPALPLPSTs-ASTDEEDDRLVRIALQRWFSRVCEDpVLIRQDEVRSFIESN 226
gi 74625805  135 VRRTHAEFKKFAKYLISTHPECLIPAVPEAKTsvSSGIKEDLIYLKSGLQSWLNYVSTNpNLLYDPELQLFVESD 209
gi 68475097  118 VRRTFNEVVKFNRYLIVSNLEVFVPVIPSAITsyPTGGEDERKKLFVEWQEWLDRITSNpILIRDEEFVYFVEND 192
gi 213402931 218 VRRTYGELCKLARYLTFMHPECLIPAVPPSVGsaGSGSIEDNRRLKESLQSWLDYVGRNpYLVTDTETKLFVESD 292
gi 156838632 115 VKKTMEEFQQLFKYLNGAIQESFIPALPIPFTnyGINNREDLDKTVHNFQLWFDRILSDpLILRNEELAFFIESD 189

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