1KMD


Conserved Protein Domain Family
PX_SNARE

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cd06897: PX_SNARE 
Click on image for an interactive view with Cn3D
The phosphoinositide binding Phox Homology domain of SNARE proteins from fungi
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. This subfamily is composed of fungal proteins similar to Saccharomyces cerevisiae Vam7p. They contain an N-terminal PX domain and a C-terminal SNARE domain. The SNARE (Soluble NSF attachment protein receptor) family of proteins are integral membrane proteins that serve as key factors for vesicular trafficking. Vam7p is anchored at the vacuolar membrane through the specific interaction of its PX domain with phosphatidylinositol-3-phosphate (PI3P) present in bilayers. It plays an essential role in vacuole fusion. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction.
Statistics
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PSSM-Id: 132807
View PSSM: cd06897
Aligned: 22 rows
Threshold Bit Score: 105.817
Threshold Setting Gi: 164660166
Created: 12-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                      ###                         ##    
1KMD_A         6 LRIKVDDVKIn----PKYVLYGVSTP--------------NKRLYKRYSEFWKLKTRLERDVGst---iPYDFPEKPGVl 64
gi 50548865   18 MDISIPTYTV-----SSPTSFTVQVDlegggdvrhrgeenKYTLTKRYSDFVALVSQLEDEVGrt---vEVALPPKSWFk 89
gi 156848280   8 VEVNIEDVKIv---nSKYASYGILIIlksvdg---siqyyDKKVYRRYSEFFKLKKNLEREFGve---iPYEFPDRLFVi 78
gi 149235963   1 MSITIPAEEEi----NGSTYYQINVKlp----------lrSYTIKKRYSDFDNLVKTLCHHIGinrkdfPYTLPAKRIHw 66
gi 213403604   1 MKVTIPTTSSssdlrAPWTWYHILIEypn---------grKMTVKRRYREFCELHETLEKQSSqfd-taVPPIPPKSWFs 70
gi 150865441   3 EIISIPTTSTh----GGATYYHVAIKlp----------lrSLTVQRRYSEFEDLVNGLCDNLGinvkdfPYELPSKRINw 68
gi 50302279    7 CEVSINDVTIv---eKRYALYHIIVNvirsk-----edysEYKSERRFKDFLQLKKQLEYECSge---lPYELPGRRLLw 75
gi 19075292    3 LKIKIPETSQssdeySRWTVYHIEVAfpn---------ggKHVVFRRFNEFVALDAQIRPNDYns---rLCKLPSKSWVs 70
gi 45191039    7 VHVTISDASVv---nKSYVQYTTRVRvqhgs-----esavEYKCRRRFSEFLQLKLDLEREFDae---iPYDFPARKFNl 75
gi 62275462    2 VEVRIPSTIVl----NDYHLYNIEIQfes--------gvhVHKLSKRFSDFVEFKKELEDTLHkt---iPYSLPSKFTAl 66
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
Feature 1                        #                                 
1KMD_A        65 drrwqrryDDPEMIDERRIGLERFLNELYNDrfdsRWRDTKIAQDFLQLS 114
gi 50548865   90 -------sKNVEFLDDRRRGLEVFLRRLVKIp---HFYSSAALLEFLQLQ 129
gi 156848280  79 wv---gsnIDPDVIESRRKKLSKFLYDLLNDsfdsKWKNSDYVSEFLDLR 125
gi 149235963  67 -------lNKSNVVEERKQEFTHFLNNMLLDl---SLRNEPEFLLFLQLP 106
gi 213403604  71 s-----tvTNSSLRESRRLQLEEFLGRLVYS----DWFHLQVVKDFLELR 111
gi 150865441  69 f-----ksNTQNIISERKAELSRFLNQAIRDs---TIQNSALLHKFLSLP 110
gi 50302279   76 srt--ansCDPDVVEERRVKLRQFLSDLLNDsfetKWRNSSLVAQFLNLP 123
gi 19075292   71 s-----tvTNEKLRESRRLALQAYVQCLSET----PWIKMPVVKKFLNIK 111
gi 45191039   76 wnm-ksrsCDPAVVDERRERLTSFLTDLLNDsfdvRWKTSPTLCAFLNMP 124
gi 62275462   67 y------qSRATTIEQRRVGLAEFLNGLLNDe---QLRTHKLVGEFLYLP 107

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