Conserved Protein Domain Family
GT_MraY_like

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cd06912: GT_MraY_like 
This subfamily is composed of uncharacterized bacterial glycosyltransferases in the MraY-like family. This family contains both eukaryotic and prokaryotic UDP-D-N-acetylhexosamine:polyprenol phosphate D-N-acetylhexosamine-1-phosphate transferases, which catalyze the transfer of a D-N-acetylhexosamine 1-phosphate to a membrane-bound polyprenol phosphate. This is the initiation step of protein N-glycosylation in eukaryotes and peptidoglycan biosynthesis in bacteria. The three bacterial members MraY, WecA, and WbpL/WbcO, utilize undecaprenol phosphate as the acceptor substrate, but use different UDP-sugar donor substrates. MraY-type transferases are highly specific for UDP-N-acetylmuramate-pentapeptide, whereas WecA proteins are selective for UDP-N-acetylglucosamine (UDP-GlcNAc). The WbcO/WbpL substrate specificity has not yet been determined, but the structure of their biosynthetic end products implies that UDP-N-acetyl-D-fucosamine (UDP-FucNAc) and/or UDPN-acetyl-D-quinosamine (UDP-QuiNAc) are used. The prokaryotic enzyme-catalyzed reactions lead to the formation of polyprenol-linked oligosaccharides involved in bacterial cell wall and peptidoglycan assembly.
Statistics
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PSSM-Id: 133467
View PSSM: cd06912
Aligned: 38 rows
Threshold Bit Score: 93.8458
Threshold Setting Gi: 154249139
Created: 17-Nov-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Mg++ bindingputative
Feature 1:Mg++ binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                ##                      
gi 145219422  43 DGVQKFHTaPTPRIGGLAIFAAlfvawlllpkgs--gmllglmliasLPAFVAGFIEDLTKrvgvrERLLATILSGVAay 120
gi 154248946  36 DYKLKTHEkATPYLGGVAFVLSfliftpys----------lfrklyiTVLSLMGLYDDIKStn-pwIRLIVEFLIGFLvs 104
gi 160903098  34 DNKLKVHKsPIPYLGGVAVFLSilpfyynd----------igfiipaSILTFIGLYDDIKSvs-pyIRLFAEFIVVSMai 102
gi 15643568   31 DGVLKPHGrETPYLGGLGIFVGvlpflwnd----------tvvllsaSIALALGLMDDLFSls-pfLRLIAEFGISLLlv 99
gi 15644297   27 PDSRKSHGrAVPPVGGVSIFLTlliferd-----------npfflfsIPLFLLGLLDDLFDls-yrIKLAVTALVAVWfs 94
gi 157363868  26 PNSRKIHTiPIPLTGGVAIFIAllvtekn-----------lltiipvVLAFSLGFLDDIEDls-ykIKLPVQFLIALSal 93
gi 150020561  27 PNKRKNHNiPTPQIGGVILFTIlsiyfn-------------fpflilLSLLIFGILDDMFKls-ykQKFTFEVLIAIYlv 92
gi 154249139  34 PSERKKHNkPVPLIGGVILFLMvlflsklgiy-----enniisdvvfYIVFAIGIIDDLLEip-yyLKLLLQFIAGIFyi 107
gi 71083272   33 PNSEKTHKnPTPAIGGVIFLLLyisiffqylifdelnyfhsklvvasLLIFFIGYYDDLKDnn-pyLKTFFFISVIFIfl 111
gi 213014709  31 AGKLKPHEtAIPYTGGTAILLSlipwlihe----------pgymvvvVSLWAIGFIDDVKGln-psLRLMIELLAGFLfs 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 145219422 121 yltgysltrvdvpg--fdvllqwmpftvaftAFAVGGVANSVNIIDGFNGLAGGVLMICFGmlaaiawqvgdthlaklci 198
gi 154248946 105 trflts------------------pveifvaTFFYAFLINSVNMMDGMDGICAITSAIAAFgliwtvmfpydk---fyli 163
gi 160903098 103 yfvggii----------------epfqffilVLTGIALVNGVNMVDGMDGVCAGTAIVSLLffslisknye-------ll 159
gi 15643568  100 wrfigld----------------nllvstfwLFFVVVLINSVNMMDGMDGLCGSLVALSALsyfflvrgvffe---nlsl 160
gi 15644297   95 tavtievsi------------fgarihpvffVIWFVGMVNAFNVVDGLDGLLSGISLFSSLmigers------------- 149
gi 157363868  94 fvvsqkitffg--------itlnntlgrvieLFWFMSMLNAINMIDGIDGLACSTTMISSLlskeys------------- 152
gi 150020561  93 wkypftlfg------------ykniftdifaIFWVVAFFNGFNMIDGFNGLSTGISIIYLStlknyn------------- 147
gi 154249139 108 nshhlyflg------------fdnlttkivtFLFFIVIVNAFNLIDGVNGLLIGIAIIYSLmtlnfe------------- 162
gi 71083272  112 ffnqeilikkilisfidvtyvlnnffsyiftILCFWLLMNSFNLTDGFNGVALSLAIIFFTslitlfdlsevd--hffii 189
gi 213014709 100 lyylnn-----------------sffeslflAFLFAAVVNAFNMIDGMDGVCSGVVVVSALvlvivfqlhp------lll 156
                        170       180       190
                 ....*....|....*....|....*....|....*..
Feature 1                                             
gi 145219422 199 lfilcvaGFMFLNFPFGkiFMGDGGAYLMGFLLAWTA 235
gi 154248946 164 slmgallAYLIYNFPPAkiFMGDMGSYTIGAILGVSV 200
gi 160903098 160 ifafailGFLLYNLPPAkiFLGDAGSYLLGFTLFYSF 196
gi 15643568  161 sllgaslGYLVFNFPPAriFMGDAGSYLMAVLLSAIV 197
gi 15644297  150 -lafsiiGFLPWNLPDAkvFLGNSGSFLLGAYLSTAS 185
gi 157363868 153 -lalavaGFLPFNLPRAksFLGNSGATLLGIYLPMRV 188
gi 150020561 148 -lalaylPIFIFNFFGKl-FIGESGVLITSYLLLLSA 182
gi 154249139 163 -iallllILLFFNFQESl-FMGDSGAFLVAYILLNSA 197
gi 71083272  190 niifflvIILIFNLFGKl-FLGNNGAYLISFILAVYL 225
gi 213014709 157 afagtffGFFVWNFPPAkiFLGDQGSYIAGAFMGMML 193

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