Conserved Protein Domain Family
GT8_GNT1

?
cd06914: GT8_GNT1 
GNT1 is a fungal enzyme that belongs to the GT 8 family.
N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases.
Statistics
?
PSSM-Id: 133064
View PSSM: cd06914
Aligned: 8 rows
Threshold Bit Score: 325.918
Threshold Setting Gi: 74606800
Created: 24-Sep-2008
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
putative ligandmetal binding
Feature 1:putative ligand binding site
Evidence:
  • Comment:annotation is based on homology to other subgroups of the superfamily

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             ###  ##                                                                    
gi 74603159   68 YAYVQYATDfDYLNLAIINFIVLRRSStkIPNLVVLFNraLQEDKNRFDGlrdls------------lrysvTLKPIPII 135
gi 74585448   71 YAYMQYATNyDYLNLAIINFIHLRKANtkIPNLVIIYDevLHYYASDKWSelyqva-----------nqykiTLKAAPLI 139
gi 74666923   80 YAYSQYATSsAYLCNAVMVFEALERLGs-RADRVLFYPedWDLFVADDHDrdsqll-----------vlakeKYKALLVP 147
gi 156062778 107 YAYITYATSkDHLCNALMLFESLQRLQs-KAERVLLYPqlWKDAWVEDVHgtdmeiisk----mliqardkyKVKLENVE 181
gi 74626254   96 YAYVNYVADkNYVCSSMIHFNRLHESGt-QAKLVMLVAkeLTELPEDDSVtrmlaqf--------keisdncIVKPVENI 166
gi 74606800   85 FAYVNYITDfEYLCNTLIQFRKLNDSGs-KAKLLALVTdtLVNKSKENKEveallnkiksvsdrvavtevgsVIQPNDHT 163
gi 74583804   92 FAYVNYVTNaDYLCNTLIIFNDLKQEFetKAKLVLLISkdLLDPNTSSNVayissllnk----iqaidedqvVIKLIDNI 167
gi 116508700 698 FAFVFYATNaGYACGALLLIKQLSSLSp-GISTVLMVPptFLPRNLVPFQrfn-----------------ttIVKLKPPP 759
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                             #                 # ###                                    
gi 74603159  136 envna------esptWSKSFTKFHIFNE----VKYDRIVYFDADSMLLHtqwndnssivnnesnvpeNLDELFtip--ei 203
gi 74585448  140 kasyq------ddsnWAASFTKFHIFNQ----VEYDRIVFFDSDSMLVDipneidfd---nmesrfnHIDELFkip--qe 204
gi 74666923  148 isaemikagggsgesWDKSIAKLLAFGE----TEYDRVIHIDSDVTVLQ------------------SMDELFflp---p 202
gi 156062778 182 ilqhrh----atadeWAESYTKLIAFNQ----TSYEKLLVLDSDSTIRH------------------PMDELFvapmsns 235
gi 74626254  167 vlsq-------gsaqWMTSMTKLRVFGM----VEYKRIVYFDSDSIITR------------------NMDELFflp--dy 215
gi 74606800  164 p--------------WSKSLTKLAIFNL----TDYERIIYMDNDAIIHD------------------KMDELFflp--sy 205
gi 74583804  168 vkpk-------dttpWNESLTKLLVFNQ----TEFDRVIYLDNDAILRS------------------SLDELFflp--ny 216
gi 116508700 760 lagg-------gdayYWSALLKLRAFQLqeqlPDLKRIIVMDSDQIIRK------------------PLHHLFhlp---e 811
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #                                                                        
gi 74603159  204 iDIALPQAYWltkhtkasgkikapdgkgyqdeitaline---------------------------isksvndslvfekl 256
gi 74585448  205 lSFASPQAYWlnnvvegksprprknveipnkkryslrmkk--------------------------lvndlsiyqdfnll 258
gi 74666923  203 aKVAMPRAYWalp------------------------------------------------------------------- 215
gi 156062778 236 tQILAPRAYWldaq------------------------------------------------------------------ 249
gi 74626254  216 iQFAAPATYWflndndlpqliednkqialannqtaelteiedilqq-------------kiddsediynflpnlpkrlyp 282
gi 74606800  206 vKFAAPISYWfvtaddlrtvstdtkklfktnkldpiekklasrvkn-------------sleiynhlpnlpqhfysksmn 272
gi 74583804  217 iKFAAPLTYWflsnsdleksyhetrhrekqpinlqsytkvltkrigkgqmiynhlpslphslylnsnniaqdiisstssl 296
gi 116508700 812 vEVAAPVAYWlen------------------------------------------------------------------- 824
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         ###                                      ##                    
gi 74603159  257 psllvqsqklnhrnnFFATHVMVVKPSnEIFNELKKYVHNPwlwslhkrhalrnksDYDMEVLNKFIDnvlqen------ 330
gi 74585448  259 psliyenhyfdnanhFFANHIMVITPSkNTFQELMRYIHNPwwwsitnrgslrkpnDYDMEILNKYLNnelqr------- 331
gi 74666923  216 ------------dtkTLSSLLIVIEPSyREFKALMESAQPAlhgqvev--dsnetqRYDMELLNNRYAds---------- 271
gi 156062778 250 ------------gipQLASHIMLIKPStSAFERLQVEFKKAgl------------gTYDMDIINSAFTeqk--------- 296
gi 74626254  283 ksdnaridstdntyfKYAATLMVIKPEqEMFERLEQEVLPKyln---------ttnKYDMDLINIEFYdfngtaeaqkkl 353
gi 74606800  273 fiididgfqksdnkvNFATHLMVIKPDvTMANDIRDNILPRylk---------akeEYDTDLINEELYnfkeliyyqfki 343
gi 74583804  297 splfdfqsskkvgklKFASNLMVINPSkEAFDEIVNVMLPKiln---------kkeKYDMDLINEEMYnlkkiiykqfif 367
gi 116508700 825 -------------teYTTSALMVISLSkRLSKEVTTALTTIrs------------kEYDMDLVNRLFYps---------- 869
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              ##                                           # ##    #    
gi 74603159  331 -------enfkvGILPHKVYGVLTGefrellhesfvseaqflp--fitgelneqwrpieiirnIKLIHFSDspiPKPWEG 401
gi 74585448  332 -------krinvGILPHRVYGVLTGefgeewherfvvepqylp--finkksnkgwspleffkkIKVVHFSDspiPKPWEE 402
gi 74666923  272 -----------aLVLPHRQYGLVTGefrkkdhrsffgn------------dyetwdpdkvlaeAKLVHFSDwplPKPWVL 328
gi 156062778 297 ---------dscGLLDHQIYALLTGefrrpitkhsgfwgk--------ghemdiwdpesvfkkSKFVHFSDwpmRKPWKL 359
gi 74626254  354 ydqspqsfkpsmLVLPFNQYTLLTKtireknrvkllsnd----------mlgyetkkptdfrdASYYHFSDspiGKPWKY 423
gi 74606800  344 frkiqylfkpsvLILPYTKYGLLTKsiddkrqkdllknai---------lgyerkekddliqdAKFIHFSDyplSKPWFY 414
gi 74583804  368 frkvrklfkpevLVLPFARYGLLTGslrnprhysiiyndvlgyktldndgndipvglndsvaySKYIHFSDyplAKPWNY 447
gi 116508700 870 ----------rmLVLPGSYCTLNSHwesselpdwmqn--------------ktkglqglweeeSQVIHFSAg--GKPWNV 923
                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|...
Feature 1                                                             
gi 74603159  402 mnnfhfynkfriychdagfdadlfnslfptswkprlttdcdsVNIWNWIMAEY 454
gi 74585448  403 enneehynikkiycdkgdme---kfhkdypvykprltddcdsVSIWNWFREQF 452
gi 74666923  329 snqkllaeilpkcd---------------fkpgtmqergcrdREVWKSLYEDF 366
gi 156062778 360 nnlewtvwapkc-------------------vpveegddcraRDIWDGLYKDF 393
gi 74626254  424 kgledipc--------------------------npgdseeiCNAWHSIFSNF 450
gi 74606800  415 snaddiqcs------------------------kkysisdenCQLWKSLYKEY 443
gi 74583804  448 psmkefecivke------------------edaedsklehqaCDLWNSVYASY 482
gi 116508700 924 nmrrmnrlreqgkvdg----------mfvkmvedwreeadktCVLGWQGRAPL 966

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap