2NXX,1R20,1Z5X,1R1K


Conserved Protein Domain Family
NR_LBD_EcR

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cd06938: NR_LBD_EcR 
Click on image for an interactive view with Cn3D
The ligand binding domain (LBD) of the Ecdysone receptor, a member of the nuclear receptors super family
The ligand binding domain (LBD) of the ecdysone receptor: The ecdysone receptor (EcR) belongs to the superfamily of nuclear receptors (NRs) of ligand-dependent transcription factors. Ecdysone receptor is present only in invertebrates and regulates the expression of a large number of genes during development and reproduction. ECR functions as a heterodimer by partnering with ultraspiracle protein (USP), the ortholog of the vertebrate retinoid X receptor (RXR). The natural ligands of ecdysone receptor are ecdysteroids#the endogenous steroidal hormones found in invertebrates. In addition, insecticide bisacylhydrazine used against pests has shown to act on EcR. EcR must be dimerised with a USP for high-affinity ligand binding to occur. The ligand binding triggers a conformational change in the C-terminal part of the EcR ligand-binding domain that leads to transcriptional activation of genes controlled by EcR. Like other members of the nuclear receptor (NR) superfamily of ligand-activated transcription factors, ec dysone receptors have a central well conserved DNA binding domain (DBD), a variable N-terminal domain, a non-conserved hinge and a C-terminal ligand binding domain (LBD).
Statistics
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PSSM-Id: 132736
View PSSM: cd06938
Aligned: 10 rows
Threshold Bit Score: 361.369
Threshold Setting Gi: 146400031
Created: 19-Nov-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
ligand bindingputativeheterodimer
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site [chemical binding site]
Evidence:
  • Structure:2NXX_e; Tribolium castaneum ligand-binding domain binds ponasterone A, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                       ###                              ## ##  #                        
2NXX_E        14 QEELIHRLVYFQNEYEHPSEeDVKRIinqpmd-----gedqcDVRFRHITEITILTVQLIVEFAKRLPGFdKLLREDQIA 88
gi 74892712  212 QEDLINKLVYYQQEFESPSEeDMKKTtpfplg----dseednQRRFQHITEITILTVQLIVEFSKRVPGFdTLAREDQIT 287
gi 146400031 325 TRELINRIVTIDQQFATPSTdMLMKVseh----------nevGNSYQQLAELTILSLQLIHQFTMHLPGFcKLSDEDKRT 394
gi 13677226  286 QEELINTLVYYQEEFEQPTEaDVKKIrfnfd------gedtsDMRFRHITEMTILTVQLIVEFSKQLPGFaTLQREDQIT 359
gi 124431281  71 QKELIHRLVYFQDQYEAPSEkDMKRLtinnqnmdeydeekqsDTTYRIITEMTILTVQLIVEFAKRLPGFdKLVREDQIT 150
gi 205371750 438 QMAVIYKLIWYQDGYEQPSEeDLKRImigspn----eeedqhDVHFRHITEITILTVQLIVEFAKGLPAFtKIPQEDQIT 513
gi 154936860 264 QEELISALVHYQEEFEQPSEaDIKRIqeigit-----dddseDPKFEHITKMTILTVELIIEFSKRLTGFgDLLREDQIT 338
1R20_D        27 QKSLIARLVYYQEGYEQPSEeDLKRVtqtwqsd---eddedsDMPFRQITEMTILTVQLIVEFAKGLPGFsKISQSDQIT 103
1Z5X_E        78 QEELIHRLVYFQNEYEHPSPeDIKRIvnaape-----eenvaEERFRHITEITILTVQLIVEFSKRLPGFdKLIREDQIA 152
1R1K_D        28 QKSLIARLVWYQEGYEQPSEeDLKRVtqtwqsd---eddedsDMPFRQITEMTILTVQLIVEFAKGLPGFaKISQSDQIT 104
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 #  ##  #       # ##            #           #                           
2NXX_E        89 LLKACSSEVMMFRMARRYDvqtDSILFVNNQPYSr---dSYNLAGmgeTIEDLLHFCRTMysMRVDNAEYALLTAIVIFS 165
gi 74892712  288 LLKACSSEVMMLRGARKYDvktDSIVFANNQPYTr---dNYRSASvgdSADALFRFCRKMcqLRVDNAEYALLTAIVIFS 364
gi 146400031 395 LHKTCKTEVLVLRTSRCYDmfdEKVVLGNEAKQWrydreQYREFIg-pLADLMFDFAHALskMQLHQAEYVLLTAIAIFS 473
gi 13677226  360 LLKACSSEVMMLRAARRYDaktDSIVFGNNYPYTq---aSYALAGlgeSAEILFRFCRSLckMKVDNAEYALLAAIAIFS 436
gi 124431281 151 LLKACSSEAMMFRVARKYDittDSIVFANNQPFSa---dSYNKAGlgdAIENQLSFSRFMynMKVDNAEYALLTAIVIFS 227
gi 205371750 514 LLKACSSEVMMLRMARRYDaatDSILFANNRSYTr---dSYRMAGmadTIEDLLHFCRQMfsLTVDNVEYALLTAIVIFS 590
gi 154936860 339 LLKACSSEVMMLRAARRYDaktNSIVFGNSYPYTe---sEYETAGlgfSAPMLFRFCRNMcrMKVDNAEYALLAAMSIFS 415
1R20_D       104 LLKACSSEVMMLRVARRYDaatDSVLFANNQAYTr---dNYRKAGmayVIEDLLHFCRCMysMMMDNVHYALLTAIVIFS 180
1Z5X_E       153 LLKACSSEVMMFRMARRYDaetDSILFATNQPYTr---eSYTVAGmgdTVEDLLRFCRHMcaMKVDNAEYALLTAIVIFS 229
1R1K_D       105 LLKACSSEVMMLRVARRYDaatDSVLFANNQAYTr---dNYRKAGmayVIEDLLHFCRCMysMMMDNVHYALLTAIVIFS 181
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                    #                   
2NXX_E       166 ERp-aLIEGWKVEKIQEIYLEALRAYVDNRrkpk--pgtIFAKLLSVLTELRTLGNQNSEMCFSLklk-nkKLPPFLAEI 241
gi 74892712  365 ERp-sLVDPHKVERIQEYYIETLRMYSENHrpp---gknYFARLLSILTELRTLGNMNAEMCFSLkvq-nkKLPPFLAEI 439
gi 146400031 474 DRp-gLLQPKSVEDIQEVYTSALQSYVDALrpk---qrnIFSRLLMKLTDLRSLTSEQSEIITGIsstsiaIKPSSSQQS 549
gi 13677226  437 ERp-nLKELKKVEKLQEIYLEALKSYVENRrlpr--snmVFAKLLNILTELRTLGNINSEMCFSLtlk-nkRLPPFLAEI 512
gi 124431281 228 SRp-nLLDGWKVEKIQEIYLESLKAYVDNRdrdt--atvRYARLLSVLTELRTLGNENSELCMTLklk-nrVVPPFLAEI 303
gi 205371750 591 DRp-gLEQAELVEHIQSYYIDTLRIYILNRhagdpkcsvIFAKLLSILTELRTLGNQNSEMCFSLklk-nrKLPRFLEEI 668
gi 154936860 416 ADrpfLREAYKVQKLQEIYLNALSAYVANKravn-sfsvMIGKLLNVLTELRTLGNLNSEMCYRIqlt-dkRLPDLLNEI 493
1R20_D       181 DRp-gLEQPSLVEEIQRYYLNTLRVYILNQnsasprsavIFGKILGILTEIRTLGMQNSNMCISLklk-nrKLPPFLEEI 258
1Z5X_E       230 ERp-sLSEGWKVEKIQEIYIEALKAYVENRrkpy--attIFAKLLSVLTELRTLGNMNSETCFSLklk-nrKVPSFLEEI 305
1R1K_D       182 DRp-gLEQPLLVEEIQRYYLNTLRVYILNQnsasprcavIFGKILGILTEIRTLGMQNSNMCISLklk-nrKLPPFLEEI 259

                 ...
Feature 1           
2NXX_E       242 WDV 244
gi 74892712  440 WDI 442
gi 146400031 550 CDV 552
gi 13677226  513 WDV 515
gi 124431281 304 WDV 306
gi 205371750 669 WDV 671
gi 154936860 494 WDV 496
1R20_D       259 WDV 261
1Z5X_E       306 WDV 308
1R1K_D       260 WDV 262

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