3BEZ,3BF0


Conserved Protein Domain Family
S49_SppA_67K_type

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cd07018: S49_SppA_67K_type 
Click on image for an interactive view with Cn3D
Signal peptide peptidase A (SppA) 67K type, a serine protease, has catalytic Ser-Lys dyad
Signal peptide peptidase A (SppA; Peptidase S49; Protease IV) 67K type: SppA is found in all three domains of life and is involved in the cleavage of signal peptides after their removal from the precursor proteins by signal peptidases. Members in this subfamily contain an amino-terminal domain in addition to the carboxyl-terminal protease domain that is conserved in all the S49 family members (sometimes referred to as 67K type), similar to E. coli and Arabidopsis thaliana SppA peptidases. Unlike the eukaryotic functional homologs that are proposed to be aspartic proteases, site-directed mutagenesis and sequence analysis have shown that members in this subfamily, mostly bacterial, are serine proteases. The predicted active site serine for members in this family occurs in a transmembrane domain. Mutagenesis studies also suggest that the catalytic center comprises a Ser-Lys dyad (both residues absolutely conserved within bacteria, chloroplast and mitochondrial signal peptidase family members) and not the usual Ser-His-Asp catalytic triad found in the majority of serine proteases. Interestingly, the single membrane spanning E. coli SppA carries out catalysis using a Ser-Lys dyad with the serine located in the conserved carboxy-terminal protease domain and the lysine in the non-conserved amino-terminal domain.
Statistics
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PSSM-Id: 132929
View PSSM: cd07018
Aligned: 137 rows
Threshold Bit Score: 149.613
Threshold Setting Gi: 197736998
Created: 25-Nov-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:active site residues [active site]
Evidence:
  • Comment:The N- and C-terminal halves of SppA in E. coli are tandem repeats and the Ser/Lys dyad is arranged such that the nucleophile Ser is located in the C-terminal half whereas the general base Lys is located in the N-terminal half.
  • Structure:3BEZ: Escherichia coli Signal peptide peptidase A (SppA)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
3BEZ_A        34 LLLDISGvivdkpdssqrfsklsrqllgassdrlqensLFDIVNTIRQAKDdrnitGIVXDLKNfag--------gdqPS 105
gi 198260263  49 LVFDMSAsiqdrpvgmtrdaf---ideaiggqrlrkisLRTLTRGIRAAASddrieGLLLTGSFavegy-----gsgfAA 120
gi 19704606   44 VLINLADsynerllks-------------nlfeddsinFYTLLQSIEAISYddrveGIILKLNGdsl---------syAQ 101
gi 162456619 346 VRVRLDStpg-------------------------vrrHTRLLQQLWRLAEdpevsGVALVLRAepa--------sslAH 392
gi 197736998  36 DKVSLKGiktvvfnlnelved----ymistvsinktlsHEAVLKALENLVNdkkieKIIIDVDEvdl---------srVH 102
gi 149920043 340 TRISLAGksd-------------------------drdLAEVVWTFDGLARrg-pgVILLEVDGsdf---------glAQ 384
gi 71019091   95 VWKVYDGsivesrgppsvsali--napaageeaprvmeMFEVIRALKWAQAddrikGILADFSGlhlpssvtpnrlglAQ 172
gi 164662519  48 TWRVFDGsivevrqaqsigqll--aqptpgdqpvrileLHQVIETLRRAKDdarirGIVADFSQmhvpravlrqplglAQ 125
gi 148259074  74 LLRRATGat---------------------------ptLEQTIAAIDRAATdprvrGIDILLGGgcc---------glTT 117
gi 114799830  46 LTLDLNAaypdqapagg------------laalsgtpgFIDLLLKLKEAESddsvkGLFIRGADygf---------gtTR 104
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                             #                                          
3BEZ_A       106 XQYIGKALKEfrd-sgKPVYAVGe----------NYSQGQYYLASFankIWLSP--QGVVDl--hGFATNGLYYKSLLDK 170
gi 198260263 121 LKEVHEAVEVfse-sgKPVWAYVv----------YPTARDMYVISAadtIYMNP--EGVVSd--tGMSMSYPYLGGFMKK 185
gi 19704606  102 SEELAHEISMara-anKKIIAYFe----------NVGRKNYYLASYaneIYMPSanSTNVNi--yPYFKENFYIKGLADK 168
gi 162456619 393 AEEVSDAIRNlrs-hgKKVLCHLe----------DAGGRSLHVCSSadaIAMNP--AGGLRf--aGMSSSYFYFGGLLNK 457
gi 197736998 103 IEEIKEIFEKls--vnKEIIAIGt----------TFDEYSYQVALLankIYMLN--TKQSSlyfrGYEYKEPYFKNILTN 168
gi 149920043 385 LEEVREAVLRyra-qgGKVVAYLe----------GASTFEYFLAAScdrIYAHP--QQGLEl--rGVLRNTFYFGELLGR 449
gi 71019091  173 IEELMQAIHEfki-akKQQHGDKarpsi-awadtFNSQGSFLLASAfdeLWMQP--AGSIPl--tGLSAQIPFFKKVLDY 246
gi 164662519 126 TEELLTALHEfr--taKQDQFGKdqpatvawsdtFDSQTAFLLATGfdrVYMQS--SGQVPl--vGLGSSLTFFRRMLQW 199
gi 148259074 118 AEELHDALARfraishRQVVARAmsf------dgAEGLGAYIVATAanrIELSD--AGDFGv--tGLALQSPFAADLLKM 187
gi 114799830 105 AEELREAIQSfka-sgKFVIAHSqgm------fgSSGPSAFHSISAadeIWMQP--GTDLMv--tGVVFETEFYKGLFEN 173
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
3BEZ_A       171 lKVSTHVFRVgtYKSAVEPFird-dxsPAAREADSRWIGELWQNYLNTVAAnrqipaeqvfpgaqGLLEGLTk---TGGD 246
gi 198260263 186 yGVGVQVTRAgdYKAAAESFvle-gmsEPSREANAAVLEDFWSEYLSVIANgag--------ieaAEYERMLn--eLGML 254
gi 19704606  169 fGVKFNIIHVgdYKSYMENLasn-tmsKEAKEDTVRVLDKNYNNFLDVVSLnrk--------inrEDLDKIIkdgeLVAA 239
gi 162456619 458 lGVQADIVRIgaHKLAAEQFare-tstRVGKDDHQALLDQLSDAFLAEVSAgrr--------ipvPELERRIa---RGPF 525
gi 197736998 169 lGVTVNTLHIgdYKVAGESFsnd--kmSEEKKESLINIKETLFQNFINLVKekr---------kvDITNEILsg-dLIFA 236
gi 149920043 450 lGVRAEFVRVaeYKGTPERAsrg-epsAPVARANRAYVTDTWNHVVRLIGHsrar-------dpaVVSEWID----RAVW 517
gi 71019091  247 fGIRVLAEARreYKSMISTFsredsltAPQIHNEAELLGELNRGLVHAIAVnrfpde--dpeltsSKVEAWMk---QGPF 321
gi 164662519 200 lGVRVLSETRneYKSVTSAFvhd-elpPEQLANLSDVLGGLQHNMARLLGQnrfseqg-pphvatAKAEHVLr---HGPY 274
gi 148259074 188 aGVEAQFEHIgkYKTYPELFtrs-gpsAAATEMMNSLAGSLYDSALVPIAArlk--------rspDQVKALFd---QAPF 255
gi 114799830 174 iDLQPQVYPFyeYKNAPNSYnet-sytEPHRMAMEALATSIWTTALEEIAEdrg--------tsaGQLRAALe---SGPK 241
                        250       260
                 ....*....|....*....|....*.
Feature 1                                  
3BEZ_A       247 TAKYALENKLVDALasSAEIEKALTK 272
gi 198260263 255 TAVDAERLGLVDELmfTDELIAALRK 280
gi 19704606  240 SSADLMNNNLIDKYvyWDNVISMVGG 265
gi 162456619 526 LAQEARQSGLVDMLayDDELGRVIDE 551
gi 197736998 237 NSEKAIQLGLIDGLstYEEIGIDYNE 262
gi 149920043 518 TPEDARRMGLIEGLawPEELDARLEG 543
gi 71019091  322 STREATQLGLINGAafKRDIIKRLID 347
gi 164662519 275 SASEALAAGLIDGIchRHDITPSLDV 300
gi 148259074 256 SAAQAKQDGLVDTVlpLSAEVAHVRG 281
gi 114799830 242 PAETALELKLVDKLgwPEDAEEAAIA 267

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