3BF0,3BEZ


Conserved Protein Domain Family
S49_SppA_1

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cd07019: S49_SppA_1 
Click on image for an interactive view with Cn3D
Signal peptide peptidase A (SppA), a serine protease, has catalytic Ser-Lys dyad
Signal peptide peptidase A (SppA; Peptidase S49; Protease IV): SppAs in this subfamily are found in all three domains of life and are involved in the cleavage of signal peptides after their removal from the precursor proteins by signal peptidases. Site-directed mutagenesis and sequence analysis have shown these bacterial, archaeal and thylakoid SppAs to be serine proteases. The predicted active site serine for members in this family occurs in a transmembrane domain. Mutagenesis studies also suggest that the catalytic center comprises a Ser-Lys dyad (both residues absolutely conserved within bacteria, chloroplast and mitochondrial signal peptidase family members) and not the usual Ser-His-Asp catalytic triad found in the majority of serine proteases. In addition to the carboxyl-terminal protease domain that is conserved in all the S49 family members, the E. coli SppA contains an amino-terminal domain, similar to Arabidopsis thaliana SppA1 peptidase. Others, including sohB peptidase, protein C and archaeal signal peptide peptidase, do not contain the amino-terminal domain. Interestingly, the single membrane spanning E. coli SppA carries out catalysis using a Ser-Lys dyad with the serine located in the conserved carboxy-terminal protease domain and the lysine in the non-conserved amino-terminal domain.
Statistics
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PSSM-Id: 132930
View PSSM: cd07019
Aligned: 3 rows
Threshold Bit Score: 258.033
Threshold Setting Gi: 144898890
Created: 25-Nov-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:active site residues [active site]
Evidence:
  • Comment:The N- and C-terminal halves of SppA in E. coli are tandem repeats and the Ser/Lys dyad is arranged such that the nucleophile Ser is located in the C-terminal half whereas the general base Lys is located in the N-terminal half.
  • Structure:3BEZ: Escherichia coli Signal peptide peptidase A (SppA)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
3BF0_A       302 SIGVVFANGaimdgee----tqgnvgGDTTAAQIRDARLdpkvKAIVLRVNSPggsvTASEVIRAELAAARaa-gKPVVV 376
3BEZ_A       302 SIGVVFANGaixdgee----tqgnvgGDTTAAQIRDARLdpkvKAIVLRVNSPggsvTASEVIRAELAAARaa-gKPVVV 376
gi 144898890  57 GIAIVPVVGtlvarsgylgaasgltgYGDIADAIEAAATdpgvRAILLDVDSSggevGGLFDLVDHIQAIRsqcgKPIWA 136
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #                                                                        
3BF0_A       377 SMggMAASGGYWISTPanyIVANPSTLTGSIGIFGVittvensldsiGVHTDGVstsplADVSi--tRALPpeaQLMMQL 454
3BEZ_A       377 SXggXAASGGYWISTPanyIVANPSTLTGSIGIFGVittvensldsiGVHTDGVstsplADVSi--tRALPpeaQLXXQL 454
gi 144898890 137 VAdeAALSAAYAIACTadrIYVTQTGEVGSIGVVAVhrdesgadaqaGLAWTFVha-gaAKVDgnphQPLSdsaRAGLQA 215
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
Feature 1                                                                  
3BF0_A       455 SIENGYKRFITLVADarhstPEQIDKIaqGHVWTGQDAKANGLVDSLgdfdDAVAKAA 512
3BEZ_A       455 SIENGYKRFITLVADarhstPEQIDKIaqGHVWTGQDAKANGLVDSLgdfdDAVAKAA 512
gi 144898890 216 DVDALYGRFVALVARcrkqpPEAIRATe-AAVYRGDQAVAAGLADKVgtlrVALADLG 272

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