Conserved Protein Domain Family
S49_Sppa_36K_type

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cd07022: S49_Sppa_36K_type 
Signal peptide peptidase A (SppA) 36K type, a serine protease, has catalytic Ser-Lys dyad
Signal peptide peptidase A (SppA; Peptidase S49; Protease IV) 36K type: SppA is found in all three domains of life and is involved in the cleavage of signal peptides after their removal from the precursor proteins by signal peptidases. Members in this subfamily are all bacterial and include sohB peptidase and protein C. These are sometimes referred to as 36K type since they contain only one domain, unlike E. coli SppA that also contains an amino-terminal domain. Site-directed mutagenesis and sequence analysis have shown these SppAs to be serine proteases. The predicted active site serine for members in this family occurs in a transmembrane domain. Mutagenesis studies also suggest that the catalytic center comprises a Ser-Lys dyad and not the usual Ser-His-Asp catalytic triad found in the majority of serine proteases.
Statistics
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PSSM-Id: 132933
View PSSM: cd07022
Aligned: 61 rows
Threshold Bit Score: 153.102
Threshold Setting Gi: 3128372
Created: 2-Dec-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:active site residues [active site]
Evidence:
  • Comment:The N- and C-terminal halves of SppA in E. coli are tandem repeats and the Ser/Lys dyad is arranged such that the nucleophile Ser is located in the C-terminal half whereas the general base Lys is located in the N-terminal half.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 144898890  57 GIAIVPVVGTLvarsgylg-aasgltgYGDIADAIEAAATdpgvRAILLDVDSSggevGGLFDLVDHIQAIRsq-cgKPI 134
gi 212702705  47 GVAVISITGVIsrkesw-----wadvgQDGIRQALDEARQdervRGILLSFNSPggvaAGVKELADYIASIDd----KPV 117
gi 46581275   71 GVAVIPVMGMLtprrswswgygyttgmLDQIRPAIASALAdrgvRAILLDVASPggtvAGMKELADYIGAERak-gtKPM 149
gi 171913618  62 NVAIIRIDGVIdkrlaaleaecfggvdLEVIDEALQQAEHdravTHVVLDIHSPgggvTGTPETAARIARLAq---tKEV 138
gi 196229320  51 GIGIIPITGVLmkrpdifsrvllgaadMDEISEAINEAGDredvQAVLLDIDSPggtvTGTPELAAAVAALSr---aKYV 127
gi 171911267  19 NVAVIPIYGVLaknagllqqvcmgfcdINPIAHAVNQAAKakdvSTIVLDIASPggqvKGIRETASAVRAAAkt-rgKTV 97
gi 153889579  62 GIAEIPVFGPIgkglgsfe-kscgatsTEDVANDITEALGrrdvSGILLNINSPggttTGVPELASAIARAAe---nKPV 137
gi 120602110  50 DVAVVNMRGTLtkrgs------wwttgMEGVRTTVQQALDdpavRAVLLAIDSPggtvDGTRELADWIAARVad-ggKPI 122
gi 83945138   93 NVALIEIEGVLmsrgfegvwsgcywpgYRDYVAAVRAANEdervDAILLRFDTPggyvAGCAEAAQALRNLNqsnggKPL 172
gi 187735829  48 SSLVIDVIGPLygdgvpln-evlggtnYQSIVKEIKGAAGn--iDEVVFIFDSPggdvQGCHETAELIKSLP-----VET 119
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 #                                                                      
gi 144898890 135 WAVAdeAALSAAYAIACTadrIYVTQTGEVGSIGVVAVHRDesgadaqaGLAWTFVHag--aAKVDGNPHQPLSdsaRAG 212
gi 212702705 118 AAYAdgLTASAAYWLASAtgrVYAPAMAQVGSVGVISEVRNisgfldkmGVSITYIAsg--kWKTAGNPVEKLTpeqTAY 195
gi 46581275  150 AAYAngLMASAAYMIGSAtgrVLAPATATVGSIGVISVYENwskwnekaGLSYAYLTag--qWKAVGNPDTPLAdneRAY 227
gi 171913618 139 HAFVncQACSAAYWLASQadvIAAAPSSCIGSIGVYMALLDetralemeGYTVNLIKag--kHKAMGASFQKLTdeeRAI 216
gi 196229320 128 YAFSdgQMCSAAYWIASQadvIFSTPSARVGSIGVLLPMLDeteafkqeGLKVDLFAag--kYKSVGVPGVALTddqRTW 205
gi 171911267  98 YAFSdeNMASSAYYLGSQaneIYVTPSATVGSIGTYLAWLDptmklameGLKLEFFGag--qHKGMGLPGKPLSegdRVL 175
gi 153889579 138 VAFTdsLMASAAYWLGSQadmIVASKSASVGSVGVYIPFLDssrraemmGLKVDVVKnkegtLKGMGIPGTSLTeaqREH 217
gi 120602110 123 ASYAdgTMCSAAYWIGGStgrVFAPAAALVGSVGVLWMHYDwsqyletnGIRATYITag--sRKAAGAPEAPLSdndKAY 200
gi 83945138  173 VGHAdeLCASAGMKLAAQcdgLFASDGAMVGSVGVRIGFFDfegalekwGERYHVYKsg--rLKDMGSPLRAPTdeeSAI 250
gi 187735829 120 VAYVkgMCCSAAYYLASAcdqVVVTETALVGSIGTVISLWNa------kSEEILTITn---dDAVFKHPETPMNteaIAY 190
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                                                                                    
gi 144898890 213 LQADVDALYGRFVALVARCRkqpPEAIRATe---AAVYRG--------DQAVAAGL-----ADKVgtlrVALADLG 272
gi 212702705 196 FQERVDALHTVFKADVAQHMgisQDPAWTE----AQILFA--------QSAQQLGL-----VTAIv--rDEDQAIN 252
gi 46581275  228 LQERLTALYRHFTDGVSASMgldAANLTTWa--dGKVFVA--------SEAPQGLVta-ivADRE----AAIATLA 288
gi 171913618 217 FQGQVDSIYADFKKAVTASRr--IADSTMQ----GQSFDG--------KTALKLGL-----VDVLt--sDSLEEYA 271
gi 196229320 206 LQSMIDEINGEFQAAVLARGr--AIDPAAMe---GQDFSG--------QKAFENSLtagvmLDRA----TVLTKMQ 264
gi 171911267 176 LQATVDEFNGWFTSAVQGAR---PKVVNATm--qGQSFSG--------ERAVGVRL-----ADGIcdtwDEFIDLL 233
gi 153889579 218 LQQRADEIFSMFKGDIKSRRp--GVKDEALr---GQTLLA--------AGSRETGLid-diGDIQha-rASLAALI 278
gi 120602110 201 FQHLIDTAYTQFLDGVSAPMgldRANPAAWa--dGQVFRA--------TEALALGL-----VTAIv--pDLAGAVA 259
gi 83945138  251 YQAEVDHLADRFYVELALGRgldLEAVRESrgweARTFTAgdppppaeLDPLAVDL-----IDAVm---TEEAAFA 318
gi 187735829 191 YRDLCNKIAGRFQEFVASGRp-gLSADAFS----GKVFVA--------EDAVSLGL-----IDDV----IPWVDFP 244

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