3BEZ,3BF0


Conserved Protein Domain Family
S49_Sppa_N_C

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cd07023: S49_Sppa_N_C 
Click on image for an interactive view with Cn3D
Signal peptide peptidase A (SppA), a serine protease, has catalytic Ser-Lys dyad
Signal peptide peptidase A (SppA; Peptidase S49; Protease IV): SppA is found in all three domains of life and is involved in the cleavage of signal peptides after their removal from the precursor proteins by signal peptidases. This subfamily contains members with either a single domain (sometimes referred to as 36K type), such as sohB peptidase, protein C and archaeal signal peptide peptidase, or an amino-terminal domain in addition to the carboxyl-terminal protease domain that is conserved in all the S49 family members (sometimes referred to as 67K type), similar to E. coli and Arabidopsis thaliana SppA peptidases. Site-directed mutagenesis and sequence analysis have shown these SppAs to be serine proteases. The predicted active site serine for members in this family occurs in a transmembrane domain. Mutagenesis studies also suggest that the catalytic center comprises a Ser-Lys dyad and not the usual Ser-His-Asp catalytic triad found in the majority of serine proteases. Interestingly, the single membrane spanning E. coli SppA carries out catalysis using a Ser-Lys dyad with the serine located in the conserved carboxy-terminal protease domain and the lysine in the non-conserved amino-terminal domain.
Statistics
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PSSM-Id: 132934
View PSSM: cd07023
Aligned: 275 rows
Threshold Bit Score: 123.752
Threshold Setting Gi: 55379667
Created: 2-Dec-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:active site residues [active site]
Evidence:
  • Comment:The N- and C-terminal halves of SppA in E. coli are tandem repeats and the Ser/Lys dyad is arranged such that the nucleophile Ser is located in the C-terminal half whereas the general base Lys is located in the N-terminal half.
  • Structure:3BEZ: Escherichia coli Signal peptide peptidase A (SppA)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
3BEZ_A       302 SIGVVFANGaixdgee-----tqgnvgGDTTAAQIRDARLDpkvKAIVLRVNSPGGsvTASEVIRAELAAARaa-gKPVV 375
gi 42522622   42 TILHLEMNGvi--------------lnGKKFIKNLKKYRDDdkvKAILISINSPGGsvGPSQELFAEIKRVRdeikKPVV 107
gi 15678828   34 TIAIIPVHGaiaydta----gfsdgvdPDDIKELIAEANSDpsvKAIVLDINSPGGtpVASEELMDAINKSE----KPVV 105
gi 15668832   58 GDGIFPQQKk----------------dARYYINLLDDLEKDdsvKGVLLVVNSPGGevIASEKLARKVEELAk--kKPVV 119
gi 24214911  289 VVAVLPLKGnihhdtigkgegktdgisYYSIKEALKELKKEssvKAVILEVDSPGGsaFVSELLYQEILKLQk--kKPVY 366
gi 150399145  50 SDSGLLSTGtt---------------gVEDYIKWLDEVENNdniKAVIIKINSPGGevVASEKLSRKIKEVSe--kKPVV 112
gi 149194025  58 YVAVININKtit------------vdyIDKITKKMDALLKDkncKEFLLVFNTPGGspSASDEFNAYLKYVNk--kKRVN 123
gi 163798488 101 STPEDDLFSeyp-------------pdVYDYIDWLDEAEKNpdiKGVIIKINSPGGssIASTKLAEKIEEVSq--kKPVV 165
gi 52548349   21 ITSEESVFGlm--------------aaSTLIIKDIEEVENKrkiKTVIFEINSPGGtpFASKEIATCIENMD----KPAI 82
gi 153896364  58 DTGRPSPLSrag------------gatADDVVEQIEAADEDedvEALVVELNTPGGevLPSDDIRRAAADFD----GPTL 121
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                #                                                                       
3BEZ_A       376 VSXGgXAASGGYWISTPanyIVANPSTLTGSIGIFGVIttvensldsiGVHTDGVsts-plADVSitRALPpe-aQLXXQ 453
gi 42522622  108 CVSTgVMASGAYYAAVAcdkIVVAPGALVGSIGVIMEFanleklydwaKISRYSItsgkfkDSGAeyRPMRed-eRSLFQ 186
gi 15678828  106 SWISdSGTSGAYLAASAsdrIVASPSAWVGSIGVILDLtdlsqmyrqmGINKYAIkageykDMGAdyRMITde-eRQMLQ 184
gi 15668832  120 VYVEgLDASGAYMVSAPadyIVAEKHSIVGSIGVRMDLmhyyglmkklGINVTTIkagkykDIGSpfRPMTke-eKEYLQ 198
gi 24214911  367 AYIQnISASGGYYLSCGaskIYASPYGIVGSIGSISIRfdlknlysklGITKDRVgfykyrDLLSeyGPIHpe-sKKLME 445
gi 150399145 113 AYIEtIGASGAYMAASSsnyIVAEKQSLVGSIGVRMELlhyyglmeklGVNTTSItggkykNIGTpnRPMTee-eYKMLE 191
gi 149194025 124 VYVEsMAASGGYYIISAikpIVANKNAVVGSIGVIMPHfvlkkladkiGIEEDSLtigkykEPVSlfRKFSdkdkEYLKT 203
gi 163798488 166 AYIEaMGASAAYGAAAStgyIVAEKEALVGSIGVRMDIlhyyglmdkiGVNSTVIkggeykDIGSpyRPMTee-eQEMLQ 244
gi 52548349   83 AWVReYGASGAYWIASAcdeIVADELSTVGSIGAMSIRpdigelmkkfGIDVETLrtgiykGMGLp-YEKPteeeRELMK 161
gi 153896364 122 AYATdTCASGGYWIASGcdeLWARDASLVGSIGVVGSRpnaagladklGISYEQFtageykDAGVplREIEed-eREYLQ 200
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1                                                                        
3BEZ_A       454 LSIENGYKRFITLVADARhstPEQIDKIAq---GHVWTGQDAKANGL--VDSLgdfdDAVAKAA 512
gi 42522622  187 SMIDEVYAQFKGTVATERklkEEVVAEYAd---GRVFTGATAVKVGF--ADQEgyfdDAVKLAA 245
gi 15678828  185 SMVNEEYDYFIKTVAANRnlsVSYVKSLAe---GRIFTGRQAVRNRL--VDFTg--gKDYAVEV 241
gi 15668832  199 KMINETYMDFVKWVAEHRhlsINYTLKIAd---GKIYSGEDAKKVGL--VDEVg--tEEDALKK 255
gi 24214911  446 QEIKESEGLFYKRVSDSRkipISDLDKRFg--qGKVFTSSQFLKERM--IDSItd-fLSLLEDI 504
gi 150399145 192 SIVDEMYFDFISMVAENRnmtINETLIAAd---GKIYNGLQAKNAGL--IDQVg--tEKDAIDM 248
gi 149194025 204 HLLLPTYENFLKTVAKDR---NISVDKLKqyadGKIYVASIVKGILVdkISTLidvkEEIKKRL 264
gi 163798488 245 KIVNNSYVDFVSQIARDRnmtYEEANELAq---GKVYDGRDALQLGL--VDENg--dFDQAINV 301
gi 52548349  162 KELDEIQDNFLNAVAKNRkldDAKMKELSt---AKVYLGREAKEMGL--IDHLg--gKELAIRI 218
gi 153896364 201 GIIDGYYEQFVETVSEGRd-mDPEAIRETe---ARVYLGSDAAEIGL--VDELg--tEDDVEDR 256

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