1ZRS,1ZL0


Conserved Protein Domain Family
Peptidase_S66

?
cd07025: Peptidase_S66 
Click on image for an interactive view with Cn3D
LD-Carboxypeptidase, a serine protease, includes microcin C7 self immunity protein.
LD-carboxypeptidase (Muramoyltetrapeptide carboxypeptidase; EC 3.4.17.13; Merops family S66; initially described as Carboxypeptidase II) family also includes the microcin c7 self-immunity protein (MccF) as well as uncharacterized proteins including hypothetical proteins. LD-carboxypeptidase hydrolyzes the amide bond that links the dibasic amino acids to C-terminal D-amino acids. The physiological substrates of LD-carboxypeptidase are tetrapeptide fragments (such as UDP-MurNAc-tetrapeptides) that are produced when bacterial cell walls are degraded; they contain an L-configured residue (L-lysine or meso-diaminopimelic acid residue) as the penultimate residue and D-alanine as the ultimate residue. A possible role of LD-carboxypeptidase is in peptidoglycan recycling whereby the resulting tripeptide (precursor for murein synthesis) can be reconverted into peptidoglycan by attachment of preformed D-Ala-D-Ala dipeptides. Some enzymes possessing LD-carboxypeptidase activity also act as LD-transpeptidase by replacing the terminal D-Ala with another D-amino acid. MccF contributes to self-immunity towards microcin C7 (MccC7), a ribosomally encoded peptide antibiotic that contains a phosphoramidate linkage to adenosine monophosphate at its C-terminus. Its possible biological role is to defend producer cells against exogenous microcin from re-entering after having been exported. It is suggested that MccF is involved in microcin degradation or sequestration in the periplasm.
Statistics
?
PSSM-Id: 132882
View PSSM: cd07025
Aligned: 203 rows
Threshold Bit Score: 158.883
Threshold Setting Gi: 62179814
Created: 8-Jan-2008
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
catalytic triaddimer interface
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic triad [active site]
Evidence:
  • Comment:The role of the catalytic triad residues is based on mutational studies.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1ZRS_B        28 VALIAPASAIAt---eVLEATLRQLEVh-GVDYHLGRHvearyr-ylagtvEQRLEDLHNAFDmpditAVWCLRGGYGCG 102
gi 16799106    8 IGIICCSDGRKkeeekNLKRLKQVLEKefGLQVVFAKTifqtddspfsgtpEERAAELMKLYQnvdvkMIFDISGGDAAN 87
gi 18311444   12 IGVISCSNGLSiknknIIEELKLNLKSl-DIEMVEGDTlyakeynlfsgtgEEKARALEKLFLdkdikMIFDISGGDLAN 90
gi 46115644   16 IAFISPSERINstlpaVVDRASAVLSNk-GYHVQTFFSedngi----qsciDNRLSEIRAAFSdpsiaAVITTIGGTTFT 90
gi 51893555   17 VGVVAPSGPVDar-reAVEAGIRRLEAy-GFRVRTGEAlwareg-vragarETQLADLHAMWAdpevkAIFCATGGVTAI 93
gi 81530503   12 VGIISCSDGIKkenkkRVESVVDILKSm-EIEAELSKCifreka-efsgsgSQRAKELMRLFLdkdikAIFDISGGNSAN 89
gi 104774095   3 AALVGCSNPLKaayrpVVDQLVKILEDr-GLEVVVSQFltddt---ligrgEKRARELESFFLdpemgHIFDISGGDLAN 78
gi 167747477   9 VGIVCCSNGLSdsarpAVMPLENALKEl-GLKPVFSRYlyagss-vfdrtgRQRAGELMEFYRnpgirAVFDISGGDLAN 86
gi 169335611   8 IGIVACSNAINikkkkEIDELISILKNi-GLIPILSNNifsdns-ifcgsgMEKAKSLMDFYNdkeikAIFDISGGDIAN 85
gi 188589180  10 VGIVACSNGIDksmelKINELERTLKSi-GFKVIFSNTiykkys-vfngngKERADELMRFFEdrsikAIFDISGGDLAN 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  #                                                     
1ZRS_B       103 QLLPg--LDWGrle--aasPRPLIGFSDISVLLSAFHrh-glPAIHGPvatglgl-------splsaprEQQERLASLAS 170
gi 16799106   88 QVLPy--LNYDiik---kaAKPFIGYSDLTVILNAIFakakqPGFNYLlrnlv------------seasEIQRVQFQKAF 150
gi 18311444   91 EVLDf--LDFNlik---enPKPFFGYSDLTVLLNAIYsqcdiTTYNYQlrnlv------------gkfkEEQMQNFKASF 153
gi 46115644   91 ELLPaliADKElheiiranPKVVVGYSDITGLHWFLYamtglRTFYGPgavpeigepndindkntplsfCVDNLLRAIAS 170
gi 51893555   94 TLVDg--LDYDlia---qnPKPFIGMSDITLLQAAMLrrtglVSLHASgladgl----------gspdaELEAPYLLRLL 158
gi 81530503   90 QVLSd--LDFNiik---nnYKPYFGMSDLTVILNSIYkktglKSYHYAiynfg------------rensRLQIDRFKKSF 152
gi 104774095  79 TVLGh--LDLEqik---dsQAVFYGYSDLTTILTALAkn-gnQAVNFQlsnc-------------lvnkDLLKSGYFDRL 139
gi 167747477  87 EVLTn--LDFQvir---ehLKPFFGYSDLTVIVNAVFqqtgrKSYLYQvrnlv------------mdhsEEQKKNFKDSL 149
gi 169335611  86 KILDf--LDYEvik---nnIKPFFGYSDLTTVINALYkkvgaVSYLYQvrnfv------------sefkEKQTRDFYESF 148
gi 188589180  88 GILEh--LNFNiir---enRKLFFGYSDLSVVINALYakcnmKTYLYQirnli------------gdysEIQIKNFKDTF 150
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                      # 
1ZRS_B       171 Vsrlla--------gidheLPVQHlg--------------ghKQRVEGaLIGGNLtALACMAGTlgglhapagsILVLED 228
gi 16799106  151 Fenqi-----------ainGKSLTd-----------------FEWSVGeVVGGNIrCFLKLAGTefmpd-vsnkIIFLES 201
gi 18311444  154 Iegke----------difnLDYKWi----------------nGSHLEGiVVGGNIrCLLKLAGTkympd-fkdkILFLES 206
gi 46115644  171 PkplgqlprslyyaprgapFFQDAasteppavvktsewqwlrPGKAQGrLFGGCLtVVARLGGIraiipdwrgrIVFLET 250
gi 51893555  159 Tnpdppgplp-dwagevrpVRPGRaesaghdr---ggasgrqDSGVRGrLVGGCCnCVLHLLGTsywpd-tegaILFLEG 233
gi 81530503  153 Iqgke----------difdFTYKWl----------------rKDVIEGeVVGGNIrCFLKLAGTeyfpn-legkVLLLEA 205
gi 104774095 140 Lagkek--------nkeldELEVTfv---------------rGSKMAGpVYGGNLrCLLKLAGTpdwpd-ftgsILLLEA 195
gi 167747477 150 Lgngn----------glfqFRTRLi----------------rGTSMEGmVVGGNIrCLLKLAGTpfwpd-mqdkILFLES 202
gi 169335611 149 Flckn----------nlfdFKYKFi----------------kGDVIQGiVIGGNIrCLLKLAGTeylpd-fqnkVLFLES 201
gi 188589180 151 Menkt----------slldFKYEWi----------------qGNNINGvVVGGNIrCFLKLAGTeyipn-fkdkILLLES 203
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1ZRS_B       229 vge-------pyYRLERSLWQLLEsida----rqlgAICLGSFTdcprk----evahsLERIFGEYaaa-ievPLYHHLP 292
gi 16799106  202 lgg-------keAKIASYVAQLEQldvf----skclGIVVGEHTeaekn----geydrIGILYQQIglk-yklPVFRTKE 265
gi 18311444  207 fsg-------nsAKMVTYITQYKNlgvf----nevkGIILGEFTemere----nlkpdIVEILKRVige-iniPILKTRD 270
gi 46115644  251 atnedgsggnppHRVQAAFADLIAqgvf----eeaaGLVVGRPYgydsdedretyagiIKGLLCEGrlaskkfPILFNVD 326
gi 51893555  234 vnl-------ltSAVLRGLAQLRLagaldgvkailfGHMEGCFRdrps------plegMALAVTEAlgd-ldlPVYQTEA 299
gi 81530503  206 lsg-------nvSNISSLVEQLSMlndf----nklsGIILGTFTqiqee----gniniLEEIILEKtkv-fniPVIKTEE 269
gi 104774095 196 yrg-------qpELVASLLEQCREtgif----nqisGVLLGTFSeldkl----kesqlPEEILLDLlpt--nlPIAKTEF 258
gi 167747477 203 rgg-------evPQMVTYLNQLKQigvf----eqvnGILLGTFTameag----gclprMEELVKNTagp--giPIAKTEE 265
gi 169335611 202 rsg-------neDVMTSYLNQLNQmgvf----dkinGVILGRFSqmesk-----vkpkVEDIVLKItsd-kdiPIVKTSD 264
gi 188589180 204 lsg-------dvAKMTTYLTHYKQlgvf----kdikGIILGNFTemenk----qyspnIIELVKEIvnd-yniPIVKTKE 267
                        330
                 ....*....|....*..
Feature 1          #              
1ZRS_B       293 SGHGAQnrawPYGKTAV 309
gi 16799106  266 IGHSVEakpcLIGAKIN 282
gi 18311444  271 LGHGADakciPIGKYLI 287
gi 46115644  327 IGHTVPmltlPYDALAE 343
gi 51893555  300 FGHCVPnvtlPIGAVAC 316
gi 81530503  270 IGHGSDskciVIGEKIK 286
gi 104774095 259 IGHRPDakaiRLGQECV 275
gi 167747477 266 IGHGTDskclIIGKKYR 282
gi 169335611 265 IGHNKDskcmIIGKEIC 281
gi 188589180 268 IGHGPDskciIIGENIE 284

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap