2HZB,3C3D,3C3D,2PPV,2Q7X


Conserved Protein Domain Family
CofD_YvcK

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cd07044: CofD_YvcK 
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Family of CofD-like proteins and proteins related to YvcK.
CofD is a 2-phospho-L-lactate transferase that catalyzes the last step in the biosynthesis of coenzyme F(420)-0 (F(420) without polyglutamate) by transferring the lactyl phosphate moiety of lactyl(2)diphospho-(5')guanosine (LPPG) to 7,8-didemethyl-8-hydroxy-5-deazariboflavin ribitol (F0). F420 is a hydride carrier, important for energy metabolism of methanogenic archaea, as well as for the biosynthesis of other natural products, like tetracycline in Streptomyces. F420 and some of its precursors are also utilized as cofactors for enzymes, like DNA photolyase in Mycobacterium tuberculosis. YvcK from Bacillus subtilis is a member of a family of mostly uncharacterized proteins and has been proposed to play a role in carbon metabolism, since its function is essential for growth on intermediates of the Krebs cycle and pentose phosphate pathway. Both families appear to have a conserved phosphate binding site, but have different substrate binding residues conserved within each family.
Statistics
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PSSM-Id: 132871
View PSSM: cd07044
Aligned: 5 rows
Threshold Bit Score: 319.142
Threshold Setting Gi: 168177315
Created: 8-Jan-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphate binding site [ion binding site]
Evidence:
  • Structure:3C3D; Methanosarcina 2-Phospho-(S)-lactate transferase with bound phosphate, 4A distance
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                      10        20        30        40        50        60        70        80
              ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          ###                                                                        
2HZB_A      6 VVVFGGGTGLSVLLRGLKTfp--vSITAIVTVADDggssgrlr-keldippPGDVRNVLVALSeveplleqlfqhr---- 78
3C3D_B      1 XIIFSGGTGTPKLLDGLKEilpeeELTVVVNTAEDlwvs--------gnliSPDLDTVLYLFSdqidrkrwwgiendtfg 72
3C3D_A      1 XIIFSGGTGTPKLLDGLKEilpeeELTVVVNTAEDlwvs--------gnliSPDLDTVLYLFSdqidrkrwwgiendtfg 72
2PPV_A      7 VVLIGGGTGLSVLARGLREfp--iDITAIVTVADNggstgkir-dvxdipaPGDIRNVIAALSdsesiltqlfqy----- 78
2Q7X_B      7 ITVIGGGTGSPVILXSLRExd--vEIAAIVTVADDggssgelrxnxqqltpPGDLRNVLVAXSdxpxfyexvfqyr---- 80
                      90       100       110       120       130       140       150       160
              ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                     
2HZB_A     79 ------fengnglsghslGNLLLAGXTSITgdf----argISEXSKVLnvrGKVLPAsnrSIILHGEXedgTIVTGessi 148
3C3D_B     73 tyerxkelgieeglklgdRDRATHIIRSNIirdgasltdsTVKLSSLFgikANILPXsddPVSTYIETa-eGIXHFqdfw 151
3C3D_A     73 tyerxkelgieeglklgdRDRATHIIRSNIirdgasltdsTVKLSSLFgikANILPXsddPVSTYIETa-eGIXHFqdfw 151
2PPV_A     79 ------rfgenqvdghslGNLVIAGXTNITndf----ghaIKELSKVLnikGQVIPStnaSVQLNAVXedgEIVHGetni 148
2Q7X_B     81 -----fsedagafaghplGNLIIAGLSEXQgst----ynaXQLLSXFFhttGXIYPSsdhPLTLHAVFqdgTEVAGeshi 151
                     170       180       190       200       210       220       230       240
              ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                ##   #                               
2HZB_A    149 p--kagkkIKRVFLTpkd----tkPLREGLEAIRKADvIVIGPGSLYTSVLPNLLVPGICEAIKQstaRKVYICNv---- 218
3C3D_B    152 igkrgepdVRGVDIRgvs---easISPKVLEAFEKEEnILIGPSNPITSIGPIISLPGXRELLKKk--KVVAVSPiigna 226
3C3D_A    152 igkrgepdVRGVDIRgvs---easISPKVLEAFEKEEnILIGPSNPITSIGPIISLPGXRELLKKk--KVVAVSPiigna 226
2PPV_A    149 p--kthkkIDRVFLEpsd----vePXNEAIEALEQADlIVLGPGSLYTSVISNLCVKGISEALLRtsaPKLYVSNv---- 218
2Q7X_B    152 v--dhrgiIDNVYVTnalnddtplASRRVVQTILESDxIVLGPGSLFTSILPNIVIXEIGRALLEtxaEIAYVCNi---- 225
                     250       260       270       280       290       300       310       320
              ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1       ##                                                                            
2HZB_A    219 -xTQNget------dgyTASDHLQAIxdhcgvgIVDDILVhgepisdtvkakyakek-aepvivdehklkalgvGTISdy 290
3C3D_B    227 pvSGPagklxpacgievSSXGVAEYYq-----dFLDVFVFderdrade------------------faferlgcHASRad 283
3C3D_A    227 pvSGPagklxpacgievSSXGVAEYYq-----dFLDVFVFderdrade------------------faferlgcHASRad 283
2PPV_A    219 -xTQPget------dnyDVKEHIDALtrqvgepFIDFVICssesyskdvlqryeekn-skpvavhkeqlkdsgiRVLTas 290
2Q7X_B    226 -xTQRget------ehfTDSDHVEVLhrhlgrpFIDTVLVniexvpqeyxnsnrfdeylvqvehdfvglcxqvsRVISsn 298
                     330       340
              ....*....|....*....|....
Feature 1                             
2HZB_A    291 fvleqd--dvlrhnaSKVSEAILE 312
3C3D_B    284 tlxt------steksKELAEIVVQ 301
3C3D_A    284 tlxt------steksKELAEIVVQ 301
2PPV_A    291 nlveisnehyvrhntKVLSKXIYE 314
2Q7X_B    299 flrlen--ggafhdgDLIVDELXR 320

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