3CGI


Conserved Protein Domain Family
BMC_PduU-EutS

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cd07046: BMC_PduU-EutS 
Click on image for an interactive view with Cn3D
1,2-propanediol utilization protein U (PduU)/ethanolamine utilization protein S (EutS), Bacterial Micro-Compartment (BMC) domain
PduU encapsulates several related enzymes within a shell composed of a few thousand protein subunits. PduU exists as a hexamer which might further assemble into the flat facets of the polyhedral outer shell of the pdu organelle. This proteinaceous noncarboxysome microcompartment is involved in coenzyme B12-dependent degradation of 1,2-propanediol. The core of PduU is related to the typical BMC domain and its natural oligomeric state is a cyclic hexamer. Unlike other typical BMC domain proteins, the 3D topology of PduU reveals a circular permuted variation on the typical BMC fold which leads to several unique features. The exact functions related to those unique features are still not clear. Another difference is the presence of a deep cavity on one side of the hexamer as well as an intermolecular six-stranded beta barrel that seems to block the central pore that is present in other BMC domain proteins. EutS proteins included in this CD are sequence homologs of PduU. They are encoded within eut operon and may be required for the formation of the outer shell of bacterial eut polyhedral organelles which are involved in the cobalamin-dependent degradation of ethanolamine. Although it has been suggested that EutS might also form hexamers and play similar functional roles in the construction of the eut organelle outer shell at present no experimental evidence directly supports this view.
Statistics
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PSSM-Id: 132886
View PSSM: cd07046
Aligned: 19 rows
Threshold Bit Score: 159.341
Threshold Setting Gi: 212696378
Created: 7-Oct-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
putativeputative
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:putative hexamer interface [polypeptide binding site]
Evidence:
  • Comment:Based on Synechocystis sp. CcmK2 (2A1B) and CcmK4 (2A10)

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                          #  #        #  ##     #    # #
3CGI_A         8 DRMIQEYVPGKQVTLAHLIANPGKDLFKKLGLQDAv--------sAIGILTITPSEASIIACDIATKSGAVEIGFLDRFT 79
gi 83596367    5 QRIIQELVPGKQITLAHIIANPDPILYQKLGLNPAvey----akaAIGVLTVSPAETAIIMADIGIKSAGVELGFVDRFS 80
gi 126651159   6 KRFIQEFVPGKQLTLSHLIANPDPEMFQKLGIQESg---------ALGIMTCTPSETVIIAGDLATKAANVRLGFLDRFT 76
gi 153809641  27 LRLTRVRVPGKSLDFAHVITPSDPAVYQNLALNIGvhegedhtgeSIGIMRVTPWEAIVVATDVAVKAADVQVGFMDRFS 106
gi 153809642   9 IRIVQETVAGKEITFAHVMGGPAPIIYQKLGLNPQvdy----rssAIGIMNMTPSESAVIASDIAVKSGNVYLGFADRFT 84
gi 153956025  13 VRIIQETVPGKQITIAHIIASPDDIIYKKLGLNPTidy----gkaAIGIVCMSPSETAIITGDIASKAANIDIGFVDRFS 88
gi 154500388  27 LRIVRVKVIGRAISLAQLIGVSDRRIYENLGLHIGthqgedhtgeSIGLLHITPWEATVAAADIAMKSGDVELGFLDRFS 106
gi 160879325   7 LRIVQELVPGKQISLAHIIAAPDPLLMEKLCFQKEner----mkaAIGILTMSPAETVIIGADLALKASGVTLQNVDYAS 82
gi 160942348  14 MRIIQESVPGKQITMAHIIASPDEVIYKKLGLDPKvdy----hrsAIGVLTLTPSETAIITADLAIKASNIQIGFIDRFS 89
gi 167040567   5 QRIIQEYVPGKQITLAHIIASPQIELCEKLGIEKKe---------AIGIMTITPAEGAIIAADIALKTSTVKIGFLDRFS 75
                         90       100       110
                 ....*....|....*....|....*....|....*..
Feature 1                                             
3CGI_A        80 GAVVLTGDVSAVEYALKQVTRTLgemMQFTTCsITRT 116
gi 83596367   81 GSVIVTGTVSEVEASIQAILQYVentLGYAVTpVTRT 117
gi 126651159  77 GSLVIVGSVSEVEMAMLEINRFLsevLGYTPSrITKS 113
gi 153809641 107 GALIILGGLSQVMTAVEEVVKFFrdeLHFDVCeIHKS 143
gi 153809642  85 GTMIITGEISDVMSAMTEVVDYFrdtLGYVVCnITKR 121
gi 153956025  89 GTLIITGGVAEVESAMKAVANYCrdkLFFTVCeITRT 125
gi 154500388 107 GAVILLGNRAEVKSALEAVLTYFreeLHFSVCdLTER 143
gi 160879325  83 GTLVFTGTVSEVEAAMNAVVEYSnrtLSFTVCdITRT 119
gi 160942348  90 GTLIITGTISDVNIAFERILEYTsseLDFTVCrITKA 126
gi 167040567  76 GSLVIIGNISAVDEAIHRIMNTLvrvLNFSPCpITKS 112

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