Conserved Protein Domain Family
Peptidase_S66_mccF_like

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cd07062: Peptidase_S66_mccF_like 
Microcin C7 self-immunity protein determines resistance to exogenous microcin C7.
Microcin C7 self-immunity protein (mccF): MccF, a homolog of the LD-carboxypeptidase family, mediates resistance against exogenously added microcin C7 (MccC7), a ribosomally-encoded peptide antibiotic that contains a phosphoramidate linkage to adenosine monophosphate at its C-terminus. The plasmid-encoded mccF gene is transcribed in the opposite direction to the other five genes (mccA-E) and is required for the full expression of immunity but not for production. The catalytic triad residues (Ser, His, Glu) of LD-carboxypeptidase are also conserved in MccF, strongly suggesting that MccF shares the hydrolytic activity with LD-carboxypeptidases. Substrates of MccF have not been deduced, but could likely be microcin C7 precursors. The possible role of MccF is to defend producer cells against exogenous microcin from re-entering after having been exported. It is suggested that MccF is involved in microcin degradation or sequestration in the periplasm.
Statistics
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PSSM-Id: 132883
View PSSM: cd07062
Aligned: 74 rows
Threshold Bit Score: 230.535
Threshold Setting Gi: 104774095
Created: 13-Jan-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
catalytic triadputative dimer
Feature 1:catalytic triad [active site]
Evidence:
  • Comment:The role of the catalytic triad residues is based on mutational studies for Pseudomonas aeruginosa LD-carboxypeptidase

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 149180696  11 DTIGIITPSTPAPvlf-seRYERGLAQLRKmGFNVIEGKCsklsqg--yrsasiRDRAEEINDFIRNpeiKAIISTIGGT 87
gi 51893555   15 DLVGVVAPSGPVDar--reAVEAGIRRLEAyGFRVRTGEAlwareg--vragarETQLADLHAMWADpevKAIFCATGGV 90
gi 87122682   11 SVIAITAFSSGISegk-kaRYQHVITGLKNqGYVIIEGDClignsq--hvsapaRQRATELMSFLLDdriDAIAAPWGGE 87
gi 149910696  11 STIAITAFSSGIEkkh-daRFRVVRENLESrGFNVVVGKClygqrk--hvsapaQKRADELMSFLMDdeiDAIYPPWGGQ 87
gi 154485118  11 DTIGVTATSSGIVnelkqkRIKNAIKNFENrGYNVKVTDNvytsdwr-gcsstgKERGEQFNELVKNkdvSCIFSVAGGD 89
gi 160878791  11 ATIGVTACSDGKPdvldavRLDHAKVQLDKfGYKVLETKNtrtsfk--nrsssaMERKEQLEELLENdevKAIIMVSGGD 88
gi 163814286  11 DTIGFVAPSFGCDtepyktAFGRAQENFRNmGFGIQIGPNcyvgegi-gisnkpELCGQEINDYYTSeknDALISCGGGE 89
gi 182434249  13 DRVGVTSPSSGVPael-reRLAVAVRDVEArGYEVVTGRCmdgagh---vsapaADRAAELTAMLTDpgiRAVVPPWGGE 88
gi 197244400  14 DLVVVTALSGQLEpge-epLLARGVAILERmGFRVRVSPLvdparprwwaagtpAEQAAELNALLRDpevRAVVAHTGGQ 92
gi 218280993  12 NTIGVCAMSSGVGhk--ldSFDESMDYIQSfGFHIVESSSvrndse---pscdaLTRVDELNALVKDssiGAVWLAAGGD 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                 #                                                      
gi 149180696  88 NSNSLLLYIDYETLr-kNPKIIMGYSDVTALLLGIYsrtglTTFYGPaivpsfge---ypemlpkGREYFENTVMQTHec 163
gi 51893555   91 TAITLVDGLDYDLIa-qNPKPFIGMSDITLLQAAMLrrtglVSLHASgladgl-------gspdaELEAPYLLRLLTNpd 162
gi 87122682   88 LAMEILPLLDFDLLkkaKPKWLFGFSDVSTLTVVISsrlnwASAHSPnlmdlaase-tealtanfLKNMATDTGGRFSqc 166
gi 149910696  88 LAIELLPLIDFAKLqtvRPKWILGFSDVSTIAAVFTsklswATGHCSnlmdltsea-ndpltantLMHLSTSIGGSFSqt 166
gi 154485118  90 YLMEMLEYVDFESIk-nNPKWFQGFSDNTTLIYPIVtkcdvAAVYGChisdfgm----kpwqkpvENALGVLEGTVKKqe 164
gi 160878791  89 YLLEMLPLLDYDKFv-kNPKWLQGYSDPTGLLYTVTvgcdiATVYGCnfsdfgm----gewhhslENNIKLLQGEAVKqd 163
gi 163814286  90 LMCEILNYIDFDRLraaDPKWFMGYSDNTNLTFLLTtlcdtASIYGPcaaafgq----epwhrsvADAMAVLQGQKLSfa 165
gi 182434249  89 TAIDLLPLLDWERLrtaEPTWVVGYSDMSTVMTPLTlltgaATVHGNnlmdtpyr--vpeglvswLDVVAAPSGHRFTqi 166
gi 197244400  93 ATIGYLDLIDLDAIr-aDPKPIIGYSDISLLHMALHaqtglVGFHADiathgfgsdwytlvdearRSELVDLYTRVLTra 171
gi 218280993  87 FQFETLPYIDFEWIe-kNPKWYLGASDPTNLLFPVTckcdlATVYGFnagsfda----ygvneysNLCLDFLKGKNIElh 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 149180696 164 phtlei-----psewteemLDWRTQDrakklvpnsgwnslrKGKTRGKLIGGNLNTMSGIIASeyfpd--------lkga 230
gi 51893555  163 ppgpl-------pdwagevRPVRPGRaesaghdrggasgrqDSGVRGRLVGGCCNCVLHLLGTsywpd--------tega 227
gi 87122682  167 aslqhtdk--wpdgtrhpaAVLAPYLktnwcw---lvaprnGSRIEGRLIGGCWDTLIHLFDTpyldlkry-aeqfdegi 240
gi 149910696 167 esklyass--wpdivteptSGIMPDTptnwkw---lvkpefGSSIEGRLIGGCWDTLSHLFETeyldlkgl-sqrysegi 240
gi 154485118 165 sfdfyes---drheyvtgfEGYCNDEkvnwvn----grgeeKIEISGRLVGGCLDVLAFLIGTkydgteqfiekykddgi 237
gi 160878791 164 sfdfymd---gfkkkgtgyEGYDSDKevcwrn----argedEVLIEGRFLGGCLDVLLNLVGTkydkttqfiekykedgi 236
gi 163814286 166 gydgwe-----keslrdeeHPFLPYNiteprv---ikgypeKFQVSGRMIGGCMDCLVNLIGTkfdhvgeflerykddgf 237
gi 182434249 167 ppgrhratgyddyaahpgvRTFTLDTpgrwtr----ldgdgDVDVEGRLIGGCVEMLSNLTGTpyldvpafagahapegl 242
gi 197244400 172 ------------------eAPGALPAngpw-------etwrPGRAQGRLFGGLLNRLVLLQATpfappa-----elfdga 221
gi 218280993 162 ssslhqd----vdfyndgaPILNTKTey-----------dgSCSVSGRLLGGCFESINDMAGTpydfveefneryksdgv 226
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #                                                                          #
gi 149180696 231 ILFIEDsfkdmAIQERSLSMLKLaGVLDDi----aGLIVGKHEhfndc---hapftFEDLLMEVmgdrqiPIMTNVDIGH 303
gi 51893555  228 ILFLEGvnlltSAVLRGLAQLRLaGALDGvkailfGHMEGCFRdrps-----plegMALAVTEAlgdldlPVYQTEAFGH 302
gi 87122682  241 ILYLENvemapADLTRSILSMKFrGVFTHi----tALLLGRSAypasnn--kedltYHEVLQRHladlgiPVLIDMDIGH 314
gi 149910696 241 VLYLENaemspTELARTILNMKFrGVFSCi----sGLLLGRSAaavpks--dqslsYYEVLEKYlmdigiPVMIDLDIGH 314
gi 154485118 238 VWVLESfnmedVVLITHLWQMKEkGYFKNa----tGFVFGRPLmyntw----ieqpYKDAVMSVlgdlnvPIIFDSDIGH 309
gi 160878791 237 VWYLESfsrdsEELFTGLWNLREaGWFQYa----kGFVFGRPCffeth----tdttYEEAILSVleelnvPVIFDVDFGH 308
gi 163814286 238 IWFLEScdlnvMSIRRAMWQMDNgGWFRYc----kGFMIGRPLqfgqe---mmgldQYQAVLGIigkynvPVVMDVDLGH 310
gi 182434249 243 LVYVEAgghdaFTICRNLHGMRLaGFFDAa----nAVLVARTSapds-----gsftQRQAVLDAlgplnvPIVADVECGH 313
gi 197244400 222 VLFWEElqrpvSRIWNDLHTLRLsGVLDRi----aGMVVGIPTevtphdgggrhvdLRDVVLDVlgqrdiPVLAQVDFGH 297
gi 218280993 227 IWFFDIfsmnsCDVYRALLKMKYmGYFKYt----kAILVGRVLfknvs----dligYEEAFKRAlp--diDVVFKMDIGH 296
                        330
                 ....*....|....*
Feature 1                       
gi 149180696 304 TFPshvFPIGIEAEL 318
gi 51893555  303 CVPnvtLPIGAVACI 317
gi 87122682  315 VPPnltLINGALAKV 329
gi 149910696 315 VPPnltLINGAIVKV 329
gi 154485118 310 KGPqfsVIMGAKAKV 324
gi 160878791 309 KPPrmaIINGAKGKV 323
gi 163814286 311 LSPmmpVVCGSMGTL 325
gi 182434249 314 VQPylpLVNGARGRV 328
gi 197244400 298 TSPnlpLPIGVRADV 312
gi 218280993 297 TYPhmyVVNGAMARV 311

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