2H5G,1VLU,1O20


Conserved Protein Domain Family
ALDH_F18-19_ProA-GPR

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cd07079: ALDH_F18-19_ProA-GPR 
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Gamma-glutamyl phosphate reductase (GPR), aldehyde dehydrogenase families 18 and 19
Gamma-glutamyl phosphate reductase (GPR), a L-proline biosynthetic pathway (PBP) enzyme that catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The glutamate route of the PBP involves two enzymatic steps catalyzed by gamma-glutamyl kinase (GK, EC 2.7.2.11) and GPR (EC 1.2.1.41). These enzymes are fused into the bifunctional enzyme, ProA or delta(1)-pyrroline-5-carboxylate synthetase (P5CS) in plants and animals, whereas they are separate enzymes in bacteria and yeast. In humans, the P5CS (ALDH18A1), an inner mitochondrial membrane enzyme, is essential to the de novo synthesis of the amino acids proline and arginine. Tomato (Lycopersicon esculentum) has both the prokaryotic-like polycistronic operons encoding GK and GPR (PRO1, ALDH19) and the full-length, bifunctional P5CS (PRO2, ALDH18B1).
Statistics
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PSSM-Id: 143398
View PSSM: cd07079
Aligned: 146 rows
Threshold Bit Score: 401.812
Threshold Setting Gi: 197784711
Created: 10-Oct-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
putative
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:putative catalytic cysteine [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2H5G_A        32 EQQGEXARSGGRXLatlEPEQRAEIIHHLADLLTDQRDEILLANKKDLeeaegr-----laapllKRLSLSTSKLNSLAI 106
gi 66363344    9 GEIARRSRESGIQLsssTVEERNAILEHFAEEIYLNRNEILEANMKDMdnsnknn----isepmkMRLLLDESKLKACIS 84
gi 116255842  13 EEKVTDAQTAALSLsnlSTARRNEALTAIADTIERNVDRILEANAADVadaekqvdageytqafvDRLTLSTSKIESIAE 92
gi 20092893    6 ETKVVKAKKASIELssvSEEVKNRALEAMAESLDRERKAILEANSKDLeyaselkkvgkltqalvDRLKVTDSKIDGMIA 85
gi 17231487   13 MKSAKRAFQASLKLgttKGIDRSRAVLAMAQALENAFDDILEANTLDLeasrema----vpelilDWLKLTPTRLEITVE 88
gi 119494530  13 TNPIRRSYAASIELaktKGEHRSRALRAMAEAIRKRQNDILEANTLDLeasrelg----vsecvsEWLKLTPERLDTTIQ 88
gi 16331508   11 VRVLDAAQGAFLALdsyGGNDRSRAVLAMAEALERSFAQILEANTLDLvvsrems----vadclcEWLKLTPERLQNTVT 86
gi 170077208  14 TAALQRAHQASLELellKGSERHLGIEAIATALEANISVILEANTKDLeasrema----vpklilDWLKLTPERLQKTVR 89
gi 145593234   6 DNILDGATRARAELptpGDERYDRYCEALADELSSGWADVLAANATDVaqgvrag----lgpelvDRLALGKRHLDALVD 81
gi 197784711   3 DALLSRARAAFDAAppvGDPAYDQYCRFLGEELVAHWPQIARANAADVaaaqrlg----mpptlvERLRLTDVHRDRMVE 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2H5G_A       107 GLRqiaassqDSVGRVLRRTRIa--kNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEaaHSNRIL 184
gi 66363344   85 SVRdvm-nleDPLGKYTLSREIts-nGLKLYRRTCPIGVILVIFEARPEVAIQISSLTIKSGNAVILKGGKEaiNTNKAI 162
gi 116255842  93 MVRsva-eqdDPLGETLTARELd--dDLNLYKVTVPIGVIGTVFESRPDALVQISALSLKSGNAVILKGGSEaeRSNRVL 169
gi 20092893   86 GIMdvi-klkDPVGETLSTLELd--nDLILYQISCPIGLIGVIFESRPDVVPQVMSLCLKSGNATIFKGGSEarESNRTI 162
gi 17231487   89 ILQrlg-eisDPLRRVRNADYQp--eDSQSYSQLMPLGVIGFIYEAFPDLGAIAAGLCIKTGNSIILKGSTEasHSNAAI 165
gi 119494530  89 ILKrlg-elpDPIGRVIGASYQl--eQGQTYYQLIPIGVVGLVYEAFPELGAIAAGLCMKTANSLILLGGSEanYSNQVI 165
gi 16331508   87 ILKrla-slpDPLQRVMASPYQf--nLAQTYCQLMPLGVVALVYESFPELAAIAAGFCLKTGNSLVLRSCGAssHSTAAI 163
gi 170077208  90 LLRqlp-klpDPLQRVKNATYQi--sGYQTYSQRMPLGVICLIHEALPELGAIAAGLCLKTANSLVIRGCGDasHTNHLI 166
gi 145593234  82 LIArtr--aaLPTVTAPTVGHRag-nGATVRRLPKPLGVVLMIFEARPTVTVEGALLPVAVGNVAILRGGTEiaATNQAL 158
gi 197784711  79 LTAmt----rDELRKVREAEVPvsgaARHVHRVPRPLGVVLMVYEARPTVTVDGALLPVAVGNAVLLRGGKEtaATNAAV 154
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2H5G_A       185 HLLTQEALsi------hGVKEAVQLVNtre--eveDLCRLdkxiDLIIPRGSSQLVRDIQKAakgIPVXGHSEGICHXYV 256
gi 66363344  163 LNCIENAInqvkknyinINREIVQMVFshe--dvnKLLSFnqfiDLVIPRGSGELVHMIKKNt-cIPVLGHADGVCMLYV 239
gi 116255842 170 YNCILEATp-------eVPSGWAQLIEare--dvqTLLEMddavDLIMPRGSSSFVQYVQDNt-sIPVLGHTEGVCHVYV 239
gi 20092893  163 FEILVKAIest----egMPKGAFQLMEtre--eimSLLSLdayvDLLIPRGSNEFVKFIQDNt-kIPVLGHTSGICHIYV 235
gi 17231487  166 VEALQHAIve-----vgLPSGCIELVTtehggslrDLITQdqyiNLVIPYGRSSLVQQVMRQa-tCPVLKSAMGNCYLYW 239
gi 119494530 166 ADTLQSAIee-----egMPIGSLEVFPneqeapiqDLVTQdqyiNLIIPYGRPHLVHQVVAQs-tVPVLRSAMGNCYLYW 239
gi 16331508  164 CEILREGLld-----adLPVDSVSHIPsetspnvqDLVGNasqlNLVIPYGRPSFVEQISQQc-tPPVLKAAMGNCYLYW 237
gi 170077208 167 AEIMQTALad-----qgLPAGCLESLSseegctvqQLVAQdrylNLIIPYGRPNMIQQVVQHa-tTPVLRTTVGNCYLYL 240
gi 145593234 159 AVVVGRALkd-----aqLPMGTVQVVTdpdrrlvrALLKRrdkiDGLIPRGSPSLIDYCLTSs-tIPVIASGGGVNHLYV 232
gi 197784711 155 ERTVHAALtr-----agLPTGLVTVLDdpqrrllrALLKRpdevDVLIPRGSPALVEHCRTSa--IPLIAGGGGVNHLYV 227
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                               #                                                        
2H5G_A       257 DSEaSVDKVTRLVRDSKCEYPAACNALETLLIHRDLlrtplFDQIIDXLRv--eQVKIHAGpkfasyltfs--------p 326
gi 66363344  240 HVDaNISKGVEIVIDGKLDYPAACNSLETLLIHKDKls-efLPALLLRLKeqgkHIKFYSDkeclkylegend----vce 314
gi 116255842 240 DSEaDLEMATAVAYDAKVQYPAVCNAVETLLVHESVap-sfLSQMMKQYRd--aGVEIRGDertqsivdtesniseeiia 316
gi 20092893  236 DEFaDPDTAWKVCFDAKVQYPAVCNAIETLLVNRKIae-afLPKMAEMYIk--aGVELRCDkdsyillaekgls--plsk 310
gi 17231487  240 SLNsSLDMVRWMILDSHESEPDPVNAIEKVLIHRQAmp-ssLAVLWNSLKe--kGFEIKGDgelveafpql-------ql 309
gi 119494530 240 SATaNVDMVRWMIFDSHQSKPDPVNAIEKVLIDPNQkg-ssLVRLWNNLRe--eGFLLKGDellvkqfpel-------aq 309
gi 16331508  238 SSKgDLEMVRQMIIDSHVGHPDPVNAIEKVLVSPGQnp-apLVRLLNNLQa--kGFKLRGDaelceqfpdhl------tl 308
gi 170077208 241 APSgDLELASWVIQDSHNYEPDAVNAIEKVLVCPEQkt-shLISLFKSLQe--qDFELRGDaslveefpehl-----api 312
gi 145593234 233 DRSaDLELAARIALDSKLGEPTACNTLELVLAHEDVae-elTRALVRAGAelaePWLLRVDprltaavppeap----vae 307
gi 197784711 228 HRSaDLDLAVRATLDSKLPEPAGCTSVETLLLDRAVar-dyLTALARGLAgregELTLHTApeldavvpctlk----tep 302
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2H5G_A       327 sevkslrtEYGDLELCIEVVdnvQDAIDHIHKYGSsHTDVIVTEdeNTAEFFLQHVDSACVFWNASTRFSDGyrfGLGAE 406
gi 66363344  315 liedlyhhEFSSNEMCVKSVssmDEAIAHINQYGShHTDVILTQdeKLAASFMNRVDSACVFHNCSSRFSDGyrfGFGAE 394
gi 116255842 317 ateadwttEYGDRIVSIAVVdslTDAIDHINMYGSkHTESILTQddNRAEQFMRAVDAASVFHNASTRFADGfrfGLGAE 396
gi 20092893  311 ateedwslEYNDLILSIKLVdtiKEAIAHINTFGShHTDGIITEnaSRRKEFIGLVDSSSVMVNASTRFADGyryGKGAE 390
gi 17231487  310 akdnewgnAYLTKTVAFKLVdslDGAIAWINQYSSgHADSIATEsyQESRQFALGVNSAATYINTSPRFSRNpsrGDSVF 389
gi 119494530 310 aeasewsqPYLNKTIAFRGVdslEEGIAWMNEYSSgHSNCLATEsySESRQFALDVNSALTFINASPRFSRHqnrGDAIF 389
gi 16331508  309 akenewgkAYLDRTVAFRTTqnlKTAIAWINSHSSgHGDCIATDsyQESRQFSMGVDSALVYVNIPPYFCRNprhGESLF 388
gi 170077208 313 gteeewqqAYLEKIVAFKRVpdlAAAITEINCNSGgHADCIVTEsyHESRQFSMGLDSALIYINASPCFSRHplgSDSVF 392
gi 145593234 308 laaaddgrEFLERAVGIRPVaglDEAVAHIRRFGSqHTEGVVAAdpVSVEAFLERVDAATLVVNGSLRLHDGptmGLGPE 387
gi 197784711 303 laahdlgrEFLDPIVAVVPVddeNAALDHVRRHGSgHTEGVIARdrHVIDAFRRRADAAMVVVNGSLRLHDGptlGLGTE 382
                        410       420
                 ....*....|....*....|....*....
Feature 1                                     
2H5G_A       407 VGISTSRIHARGPVGLegLLTTKWLLRGK 435
gi 66363344  395 IGISTTRIHARGPVGLegLLTYKYTLRGQ 423
gi 116255842 397 VGISTGKIHARGPVGLdgLTTYKYHLEGD 425
gi 20092893  391 VGISTNKIHSRGPVGMegLLIYKYILMGK 419
gi 17231487  390 LGMSNQKGHRRGFISLetLTTLKHIIQGN 418
gi 119494530 390 LGMSNQVGYRRGLIGLdsLTTLKYIVQGN 418
gi 16331508  389 LGVSSQKGQRRGLIGLeaFMTPKQIVQGE 417
gi 170077208 393 LGVSNQKGNRRGLIGLesFTTIKQVVQGD 421
gi 145593234 388 LSIATGRLHVRGPVSLsaLLTWSWVVDAG 416
gi 197784711 383 IAIATGRLHVRGPVTLgaLVTYSYVTDGA 411

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