1UXU,2ESD


Conserved Protein Domain Family
ALDH_F11_NP-GAPDH

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cd07082: ALDH_F11_NP-GAPDH 
Click on image for an interactive view with Cn3D
NADP+-dependent non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase and ALDH family 11
NADP+-dependent non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase (NP-GAPDH, EC=1.2.1.9) catalyzes the irreversible oxidation of glyceraldehyde 3-phosphate to 3-phosphoglycerate generating NADPH for biosynthetic reactions. This CD also includes the Arabidopsis thaliana osmotic-stress-inducible ALDH family 11, ALDH11A3 and similar sequences. In autotrophic eukaryotes, NP-GAPDH generates NADPH for biosynthetic processes from photosynthetic glyceraldehyde-3-phosphate exported from the chloroplast and catalyzes one of the classic glycolytic bypass reactions unique to plants.
Statistics
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PSSM-Id: 143401
View PSSM: cd07082
Aligned: 29 rows
Threshold Bit Score: 619.587
Threshold Setting Gi: 94265647
Created: 2-Jan-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:NADP binding site [chemical binding site]
Evidence:
  • Structure:1UXU; Thermoproteus Tenax NP-GAPDH with cofactor NADP bound, defined using 3.5 A.
    View structure with Cn3D
  • Structure:2ESD; Streptococcus mutans NP-GAPDH thioacylenzyme intermediate with reduced cofactor nicotinamide moiety, defined using 3.5 A.
    View structure with Cn3D
  • Comment:The structure reveals that reduction of the cofactor upon acylation leads to an extensive motion of the nicotinamide moiety with a flip of the reduced pyridinium ring away from the active site.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1UXU_A        18 VYPSYLaGEWGgsg-qeIEVKSP---------IDLATIAKVIsps---reEVERTLDVLFKRGRWSARdmPGTERLAVLR 84
gi 18977127   23 EFKTYIaGKWIyge-kfKDIKSP---------INGEVIARVSils---reQIEEAISTTYEIGREKIRdyPGEKRISSFL 89
gi 91201212   28 QREYLCnGTIKnwtgprQDVLSPicdm-hngtIQQRRIGSYPllteqqaiETLEAACQAYDHGRGTWPtmSIAERITHVK 106
gi 124003968  29 QREYLIdGELRqwqgpmNQVQSPvcie-tmegYQQKIIGSSPlltskeslEALTAAHKAYNKGQGFWPtlKVKERIQYME 107
gi 191161099  27 QDYYLInGELRrwdgplQEVVSPvqvk-seglLENERVGEAPllsveaalEALDVAVSAYHNGRGTWPtmSVGERIHCIT 105
gi 172055273  29 QKEYLVnGKLEtwegetEESLSPiylk-teqeLVRQTIGTFPslnqeaalSTLEAAVTAYNHGRGKWPtmSVSQRIECVQ 107
gi 71907695   29 QRAILInGEMRiwkgetRTVHSPvcvqgsdgsLSHVELGSVPvtgtveadAALAAAVTAYDHGRGAWPnlSVAERIACVG 108
gi 120435093  17 QETYLSnGELIkwkgktDEVYSTiss---tddYKPTLLGTVPhmdektalEVLNSACDAYAKGQGDWPtmKVADRISCME 93
gi 150398918  25 QNKYLInGELCkwqgdfQNVYSPiyvr--tenNSSNLIGSYPlmdketalYALESAVNAYNNGQGEWPrmPIEQRIDCLK 102
gi 15597519   29 QRDYLVdGQLRhwdgplAPVRSPvfla-eadgERQVILGSTPlldadaalAALDAAVRAYDHGQGLWPnlRVAERIQHVE 107
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       #
1UXU_A        85 KAADIIERNLDVFAEVLVMNAGKpksAAVGEVKAAVDRLRLAELDLKKigGDYIPGDwtydtleTEGLVRREPLGVVAAI 164
gi 18977127   90 KAAEMMKEAFDDFVRILILDAGKpksNAMGEVKATIERLEKTTFEFGRliGDYIPGDwseetleSEAIVKREPYGLILAI 169
gi 91201212  107 GFTGRMKERRADVVKLLMWEIGKtlpDAQKEFDRTVDYITDTIDALKDldRVSSRFVie---qgVIGQIRRVPLGVVLCM 183
gi 124003968 108 VFVTEMQKQRDEVVKLLMWEIGKtlaDSTKEFDRTVTYIQDTINALKDldRRSSKFEie---sgVIAQIRRVPLGVTLCM 184
gi 191161099 106 RFSRRVQEKRDEVVKLLMWEIGKslkEAQGEFDRAIAYIRDTVEALKEldRVSSRFViq---qgIIGQIRRAPLGVVLCM 182
gi 172055273 108 EFAYRMKEKRDTVVTLLMWEIGKsytDSCKEFDRTVNYIQDTIDALKNldRVSSRFEis---qgVIGQIRRAPLGIVLSM 184
gi 71907695  109 DFTNQIVARRREIVNLIMWEIGKslaDSQKEFDRTIDYIRATVEELKRldNSNSRFEiv---dgTIAQIRRSPVGIALCM 185
gi 120435093  94 DFVEQMKTKREKVVKYLMWEIGKslpDSQKEFDRTVEYIKDTIEDYKKldRDSAKFHek---dgVYAHIRRGPLGVVLCL 170
gi 150398918 103 NFVSLMKEQKNLVVNLLMWEIGKtlkESEKEFDRTIEYINDTIEALKDldRDASRITik---qgIIAQIKRAPLGVVLCM 179
gi 15597519  108 RFLARMREQREAVVKLLMWEIGKnlkDSEKEFDRTCDYIVDTIEALKEldRHSSRFEle---qgTLGQIRRVPLGVALCM 184
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ###                       # ##                             #    #            # #
1UXU_A       165 TPFNYPLFDAVNKITYSFIYGNAVVVKPSIsdPLPAAMAVKALLDaGFPPDAIALLNLPGKe-AEKIVADDrVAAVSFTG 243
gi 18977127  170 SPFNYPLFISATKVIPALLSGNAVLLKPASadPLAPILFTRVLELsGLPKESFSLLTVPGGf-MDDIVRDKrIRAITFTG 248
gi 91201212  184 GPFNYPLNETFTTLIPALIMGNVVIFKPPKlgVLLHHPLLEAFRD-SFPKGVVNTVYGKGQrvIGPIMKSGkVDVLAFIG 262
gi 124003968 185 GPYNYPLNETFTTLIPALIMGNTVVFKPAKygVLLIRPLLKAFCD-IFPKGVINIIYGRGRdtVGALMETGqVDAFAFIG 263
gi 191161099 183 GPYNFPLYETFTTLIPSLVMGNTILLKPPRfgILLFAPLLEAFRD-CFPPGVVNTVYGDGEevLPPLMASGrIDVLGFIG 261
gi 172055273 185 GPANYPLNETFTTLIPALIMGNTVIVKAPRpgVLLNYPLLEAFKD-AFPPGVVNTIYGKGRtiTPPLMESGkIDALAFIG 263
gi 71907695  186 GPYNYPMNETFSTLIPALIMGNVVLFKPPRfgVLLYYPMLEAFRS-AFPPGVINIVYGQGHvvVPHIMGSGqVNVLALIG 264
gi 120435093 171 GPYNYPLNETFALLIPALIMGNTVVFKPAKhgVLLLSPLLEAFQS-SFPKGVVNVIYGRGRvvAAPIMQTGrIDVLALIG 249
gi 150398918 180 GPFNYPLNETFTTLIPALIMGNTVVFKPAKlgVLLLSPLLNAFKS-AFPKGVVNIVYGRGRnvIEPIMTSGkINVLAFIG 258
gi 15597519  185 GPYNYPLNETFTTLIPALIMGNTVVFKPAKfgVLLIRPLLEAFRD-SFPPGVINVIYGRGRetVSALMASGkVDVFAFIG 263
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #  #                                                                            
1UXU_A       244 STEVGERVVKVGg---VKQYVMELGGGDPAIVLEDaDLDLAADKIARGIYSYaGQRCDAIKLVLAErpvYGKLVEEVAKR 320
gi 18977127  249 STEVGEHIIRTGg---IKAYHLELGGKDPAIVLNDaNIEESVEKIVKGIVTYsGQRCDAIRLILVEediYEQFRESLVAQ 325
gi 91201212  263 SSRVADILKQQHpkphRLRCVLGLEAKNAGIVLKDaDLDLAVKECVLGSLSFnGQRCTALKMLFVHkeiADAFITKFSDA 342
gi 124003968 264 TNKGANDLKKMHpkphKLRSVLGLDAKNPGIVLPDaDMENAVAECVTGSLSYnGQRCTALKILFVHrsiVEEFLGKFTEA 343
gi 191161099 262 THKVADALRAIHprphRLRCVLGLDAKNPAIVLPDaDLELAVEECLSGALSFnGQRCTALKIIFVHksiAGEFIKGMAAG 341
gi 172055273 264 GSRAANNLKKQHpkshRLRSILGLEAKNPGIVLPHaDLDLTVKECILGTLSYnGQRCTAIKIIFVHrsvIDTFLEKFTVA 343
gi 71907695  265 SSEVADQLKKSHpktnRLRAILGLGAKNAAIIMPDaDIELTVRECITGALSFnGQRCTAIKMILVHqsiAETFLRRFCEE 344
gi 120435093 250 NSKSAIALQDQHpyknRLRLVLGLEAKNPGIILPDaDLDLAIEECISGTLSFnGQRCTALKILYVHeevQEEFNKRFAKR 329
gi 150398918 259 SCNVANTLKKQHpyphLLKSVLGLEAKNPAIILSDaEIEHTIKECITGTLSFnGQRCTALKILFIHekiYDIFLEKFTSA 338
gi 15597519  264 THSGASDLKKLHprphRLRAALGLDAKNPGIVLPQvDLDNAVNEAVTGALSFnGQRCTALKILFVHesvLRPFLDKFSAR 343
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                      # 
1UXU_A       321 LSSLrVGDPRDPt-VDVGPLISPSAVDEMMAAIEDAveKGGRVLAGGRRlg---pTYVQPTFVEAPadrvkdMVLYKREV 396
gi 18977127  326 LSKIePKNPLEDenAIMGPLIDEDSANYIEEIYKDAieKGARPLLEFRRr----gNYVWPIVLEVNneilptLRAFNEDV 401
gi 91201212  343 VDRLkSGMPWDEg-VQITPLPEPNKTTYLTELIEDAcsKGARVVNKDGGvv--rqTFIVPAVVFPVgp---gMRLFSEEQ 416
gi 124003968 344 VNHLkPGMPWDEg-VKLTPLPEPGKTDYLAGLVKDAerHGAQVVNAGYGggevneTYFHPAILYPVnd---qMRVYREEQ 419
gi 191161099 342 IAALpCGVPWQEg-VAITALPEPEKPEYLEGLLMDAeaHGARVVNEGGGar--sgSYFFPALLYPVtp---kMRIYQEEQ 415
gi 172055273 344 VSELkFGMPWEDn-VFLTPIAEPGKPNYLQELVEDAknHGAEVINEGGGmi--neTFFYPAIVYPVnd---kMKIYSVEQ 417
gi 71907695  345 VGKLaIGMPWEAg-VTLTPLPEMAMVTYMNECIADAlsKGAKVINPGGGtt--veTLFYPAVIFPVse---gMKLYREEQ 418
gi 120435093 330 VDELkFGNPWNEn-VMLTPLPEPDKPKYIQELIDDAqkKGAKILNERGGat--seNYIFPAVLYPVte---dMQVYKEEQ 403
gi 150398918 339 VDNLnLGLPWDKn-VDLTPLPEKDKPMLLQEILEDAikKGAIICNKNGGiv--neTSFFPVVLSNVne---tMRIYHEEQ 412
gi 15597519  344 LASLkPGMPWEPg-VALTPLPEPGKVDYLEGLLNDAlaKGARVVNAGGGeh--rqSFFYPALLSPVsp---dMRLYHEEQ 417
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1UXU_A       397 FAPVALAVEVKdLDQAIELANGRPYGLDAAVFGRdVVKIRRAVRLL--EVGAIYINDMprHGIGYYPFGGRKKSGVFREG 474
gi 18977127  402 FGPLALLIKVKdEDEAIEIANSSRFGLDAAVFTTcEKRARKVARKL--EVGSVFINEYprHGIGYYPFGGMKDSGIGREG 479
gi 91201212  417 FGPVVPIATFSnIEEPMNYVIQSDYGQQVSLFGSnTNLIGEIIDTMvnQVCRVNINSQcqRGPDTFPFTGRKDSAEGTLS 496
gi 124003968 420 FGPVVPVVAYDnIEEVIAYVVSSNFGQQASIFGRdPQKIAELIDELyhQVSRININAQcqRGPDVFPFNGRKDSAEGTLS 499
gi 191161099 416 FGPVVPVVVYEdIKETIDYVVNSNYGQQASIFGQdDELLAQLIDALvnQVCRININSKcqRSPDSFPFNGRKNSAEGTQS 495
gi 172055273 418 FGPVVPIVPFEdIETPINYLVNSDYGQQVSIFGQnTEAIAQLVDPLvnQVCRVNLNSQcqRGPDTFPFTGRKDSAEGTLS 497
gi 71907695  419 FGPIIPVATFEdIETPLEYVITSDHGQQVSIFGSdPGQIASLVDTLvnQVCRVNINCQcqRGPDVFPFVGRRDSAEGTLS 498
gi 120435093 404 FGPVIPIKPFNsIEKLLDEISDSNYGQQVSLFGTgIDKIAPLIDTLvnLVCRVNLNSScqRGPDVFPFTGRKDSAVGTLS 483
gi 150398918 413 FGPIIPIVKFNdIQEPLKYVINSNFGQQASIFGNdAIQISKLIDALvnQVCRVNINSQcqRGPDIFPFTGRKDSAEGTLS 492
gi 15597519  418 FGPLVPVVPYReLDEVIDYVLRSDYGQQLSLFGNdPAQIGRLVDAFanQVGRINVNAQcqRGPDSYPFNGRKNSAEGTLS 497
                        490
                 ....*....|....*..
Feature 1                         
1UXU_A       475 IGYAVEAVTAYKTIVFN 491
gi 18977127  480 IGYSLEMLTTTKTIVHN 496
gi 91201212  497 VSDALRVFTIRTLVAAK 513
gi 124003968 500 VSDALRVFSIRAIVATK 516
gi 191161099 496 VADALRVFSIRIVVAAR 512
gi 172055273 498 VHDALRAFSIRTLVAAK 514
gi 71907695  499 VHDALRAFSIRTMVAAK 515
gi 120435093 484 VHDALRSFSIRTFVASK 500
gi 150398918 493 VSDALRVFSIRTMVAVK 509
gi 15597519  498 VHDALRVFSIRTLVATR 514

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