2O2Q,1BXS,1CW3


Conserved Protein Domain Family
ALDH_F1-2_Ald2-like

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cd07091: ALDH_F1-2_Ald2-like 
Click on image for an interactive view with Cn3D
ALDH subfamily: ALDH families 1and 2, including 10-formyltetrahydrofolate dehydrogenase, NAD+-dependent retinal dehydrogenase 1 and related proteins
ALDH subfamily which includes the NAD+-dependent retinal dehydrogenase 1 (RALDH 1, ALDH1, EC=1.2.1.36), also known as aldehyde dehydrogenase family 1 member A1 (ALDH1A1), in humans, a homotetrameric, cytosolic enzyme that catalyzes the oxidation of retinaldehyde to retinoic acid. Human ALDH1B1 and ALDH2 are also in this cluster; both are mitochrondrial homotetramers which play important roles in acetaldehyde oxidation; ALDH1B1 in response to UV light exposure and ALDH2 during ethanol metabolism. 10-formyltetrahydrofolate dehydrogenase (FTHFDH, EC=1.5.1.6), also known as aldehyde dehydrogenase family 1 member L1 (ALDH1L1), in humans, a multi-domain homotetramer with an N-terminal formyl transferase domain and a C-terminal ALDH domain. FTHFDH catalyzes an NADP+-dependent dehydrogenase reaction resulting in the conversion of 10-formyltetrahydrofolate to tetrahydrofolate and CO2. Also included in this subfamily is the Arabidosis aldehyde dehydrogenase family 2 members B4 and B7 (EC=1.2.1.3), which are mitochondrial, homotetramers that oxidize acetaldehyde and glycolaldehyde, as well as, the Arabidosis cytosolic, homotetramer ALDH2C4 (EC=1.2.1.3), an enzyme involved in the oxidation of sinapalehyde and coniferaldehyde. Also included is the AldA aldehyde dehydrogenase of Aspergillus nidulans (locus AN0554), the aldehyde dehydrogenase 2 (YMR170c, ALD5, EC=1.2.1.5) of Saccharomyces cerevisiae, and other similar sequences.
Statistics
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PSSM-Id: 143410
View PSSM: cd07091
Aligned: 25 rows
Threshold Bit Score: 742.489
Threshold Setting Gi: 46122907
Created: 14-Dec-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
NAD(P) bindingcatalytic
Conserved site includes 25 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:
  • Structure:2O2Q: rat 10'formyltetrahydrofolate dehydrogenase in complex with NADP+, defined using 3.5 A.
    View structure with Cn3D
  • Structure:1CW3; Human mitochondrial aldehyde dehydrogenase (ALDH2) complexed with NAD+ and Mn2+, defined using 3.5 A.
    View structure with Cn3D
  • Structure:1BXS; Sheep liver cytosolic aldehyde dehydrogenase (ALDH1) complexed with NAD+, defined using 3.5 A.
    View structure with Cn3D
  • Citation:PMID 9195888
  • Citation:PMID 9862807

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2O2Q_A        34 QMPYQLFIGGEFVDAEGSKTy-nTINPTDGSVICQVSLaqvsDVDKAVAAAKEAFENglwgkiNARDRGRLLYRLADVME 112
gi 66803593    6 LPNTKLFINNEWVESVSGKKl-kTFNPTNGKLICEVSEggkeDVDVAVKAARNALDNgpwgkmTAEDRGKLILKLADLVD 84
gi 118361802  10 KFQTKLFINGKFVDGALKKTi-pVINPATEELICEIAEateqDVELAIDAAEASFPIws--klPQRDRTEYLLKLASLLE 86
gi 150866913  18 TQPTGLFINNEFVKSVSGKTl-dSINPSTSEVNGTVYCaeeeDVDIAVKAARAAFKDwk--kvTGVDRGILLNKVADAFE 94
gi 20981689   23 EQPTGLFINNKFMKAQDGKTy-pVEDPSTENTVCEVSSatteDVEYAIECADRAFHDtewatqDPRERGRLLSKLADELE 101
gi 50551783   37 EQPVGLFINGEFVKSQSGKRf-eTENPTTETPIISVYEageaDANAAVEAAKNAFKNwgf-ktAPSERGVLLNKLADLIE 114
gi 66805929   14 TIPTKIFINNEWVDSIDCNEnfsLINPTNEECLGIIGLggrkDVDRAVEAARSAIRGkws-tmAPLDRGILLNKLADKLE 92
gi 119175659  17 EIPTGLFINNEFVPSLTGTTl-dTTNPANGDLLATIAAaseeDINAAVQSAKDAFYTtwk-atPPTTRQSLILKLADLIE 94
gi 46122907   15 QIPTGLFINNRFIPSTTSETl-tTENPTTNTPLGQVSAaqpsDVDAAVSSSKEALKTwk--tsQPAERRRLLNRLADLIE 91
gi 2506349    42 EQPTGLFINNKFVPSKQNKTf-eVINPSTEEEICHIYEgredDVEEAVQAADRAFSNgswngiDPIDRGKALYRLAELIE 120
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                     ## 
2O2Q_A       113 QHQEELATIEALDAGAvytlALKTHVGMSIQTFRYFAGWcdkiqGATIPinqa-----rpnrNLTLTKKEPVGVCGIVIP 187
gi 66803593   85 QHREHLSDLETLDNGKpltaSSGFDITEAARALRYFGGWadkiqGKTIPis---------seFTAMTKHEPIGVVGLIVA 155
gi 118361802  87 ANKEEFIALESLDNGKpl-eGATFDINDVIGHLRYYAGWadkitGKSFSsl---------dqQIFYTRREPYGVVGLISP 156
gi 150866913  95 AQRDLIGAIEAWDSGKtkeqNAVYDIDECISCFRYFAGWadkiqGKVIQnd---------pkKLAYTIHEPLGVCGQIIP 165
gi 20981689  102 SQIDLVSSIEALDNGKtl-aLARGDVTIAINCLRDAAAYadkvnGRTINtg---------dgYMNFTTLEPIGVCGQIIP 171
gi 50551783  115 RDLDLISAIETTDNGKvf-aQAQGDVALVVKVLRYYAGFadkigGDLVQtn---------dgFFNYTRKEPLGVCGQIIP 184
gi 66805929   93 EKREQMATIESINVGKpigeSLVYDLKQSITFLRYFAGWadkitGRTIPissssdtstptrqVLAYTKQVPLGVCALILP 172
gi 119175659  95 RDVDEFANLEALDGGIllsgSKGMHVPHSVETLRYFAGWadkidGKSIAip----------sGIAYTHREPLGVCAAIVP 164
gi 46122907   92 RDARELASIEAIDAGMlfnmSLGFCIVQAVETLRYFAGWadkidGQSLEfd----------qGLAYTKREPIGVCAAVVP 161
gi 2506349   121 QDKDVIASIETLDNGKai-sSSRGDVDLVINYLKSSAGFadkidGRMIDtg---------rtHFSYTKRQPLGVCGQIIP 190
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ##                      #  #                            ##   ##            #### 
2O2Q_A       188 WNYPLMMLSWKTAACLAAGNTVVIKPAQvtPLTALKFAELTLkAGIPKGVVNILPGSGslvGQRLSDHPdVRKIGFTGST 267
gi 66803593  156 WNFPLLLLSWKLGPSLAAGCTIVAKPSEftPLTALYFCELVKeAGFPPGVINIVNGVGdvvGDALSHHMdVDKISFTGST 235
gi 118361802 157 WNFPLMMAEWKYAPALAAGNCIVLKPSEvtPLTVLRLCELVNeAGFPPGVFNVVPGYGhvaGVAITHSKrISKISFTGSS 236
gi 150866913 166 WNYPLAMAAWKLAPALAAGNVVVLKTSEitPLSLLYVARLFKdAGFPAGVVNIISGFGavaGKALSSHLdVDKIAFTGST 245
gi 20981689  172 WNFPIMMLAWKIAPALAMGNVCILKPAAvtPLNALYFASLCKkVGIPAGVVNIVPGPGrtvGAALTNDPrIRKLAFTGST 251
gi 50551783  185 WNFPLLMWAWKIAPALTTGNTVVLKTAEstPLSALYACKLSQeAGFPKGVLNVVSGYGp-vGGVLSAHPdIKKIAFTGST 263
gi 66805929  173 WNFPLQLLMFKLAPALAAGNTVIIKPSEftPLSTFYLAELIKeVGFPPGVVNVVCGLGsvvGDAMSSHMkINKIGFTGST 252
gi 119175659 165 WNAPLIVTMWKLAPCIAAGNTLVLKTSEltPLYGIKLAALIKeAGFPAGVVNIVTGLGavaGRALSEHMdVRKIAFTGST 244
gi 46122907  162 WNTPLLITAWKLGPALAAGNTLIIKTPElaPLYGQKLAQLVLeAGFPPGVVNIITGLGpvaGQALADHHqVRKISFTGSL 241
gi 2506349   191 WNFPLLMWAWKIAPALVTGNTVVLKTAEstPLSALYVSKYIPqAGIPPGVINIVSGFGkivGEAITNHPkIKKVAFTGST 270
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #  ##              ###                                #                        
2O2Q_A       268 EVGKHIMKSCAlsnVKKVSLELGGKSPLIIFADc-DLNKAVQMGMSSVFFNkGENCIAAGRLFVEesIHNQFVQKVVEEV 346
gi 66803593  236 RVGRLIMEAAAksnLKPVTLELGGKSPNIIFGDc-DIEYVANAAKNYVFANsMQLCCAASRFFVHesILDAFLTIFTEKI 314
gi 118361802 237 FVGRKILEASSqtnLKKVVLELGGKSPVVVFPDa-DLDKAAEVAWYGCMFNmGQSCDAGTRLFVHedVYDKLLQKLKEYE 315
gi 150866913 246 ATGKLIQQAAAs-nLKAVTLECGGKSPLIIREDa-DLEQAVKWAAIGIMSNqGQICTSTSRVYVHesVYDKFLEEYTAHV 323
gi 20981689  252 EVGKSVAVDSSesnLKKITLELGGKSAHLVFDDa-NIKKTLPNLVNGIFKNaGQICSSGSRIYVQegIYDELLAAFKAYL 330
gi 50551783  264 ATGKQVAKTALtsnLKKTTMELGGKSPNIIFDDa-NLEDALSAAALGIFFNsGEVCCAGSRLFVQagVYDQVVEAFKKKA 342
gi 66805929  253 KVGKMVQNSATnsnLKHCSLELGGKSPIIIFNDveDLDLAVIHSFHGLFWNaGQCCSAASRIYVQsgIYDQFVEKIKKQV 332
gi 119175659 245 LTGRAIMKAAAssnLKKVTLELGGKGPTIIFDDa-DLQNAVFWATLGITANnGQICVAGSRIYVQegIYEKFIAAFSAAS 323
gi 46122907  242 AVGRTILVSAAksnLKRVTLELGGKGPSIIFNDa-NFENALAFATAGITMHnGQICAAGSRIYVQadVYDRFVSEFAAKT 320
gi 2506349   271 ATGRHIYQSAAa-gLKKVTLELGGKSPNIVFADa-ELKKAVQNIILGIYYNsGEVCCAGSRVYVEesIYDKFIEEFKAAS 348
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                      # 
2O2Q_A       347 EKMkignp-lerdtNHGPQNHEAHLRKLVEYCQRgv-keGATLVCGGNQvp----rpGFFFQPTVFTDvedhMYIAKEES 420
gi 66803593  315 KQLkvgdp-ydsetHLGPLVSKQQHDRVLGYIQKgk-eeGATCHLGGEVhnhhadgaGYFIQPTIFTNvtddMTICKEEI 392
gi 118361802 316 QNVvigdsfevqnsNHGPLVSKLQFDRVMSYITHakeveKLQCFMGGERwg----nkGYFIKPTIFINvddnSKLAREEI 391
gi 150866913 324 KEAykqgsmfdseaVVGPQVSKVQRDKVLSYIEIgk-keGARLLLGGEKnsegelskGFYIKPTIFADikpeMRIVNEEI 402
gi 20981689  331 ETEikvgnpfdkanFQGAITNRQQFDTIMNYIDIgk-keGAKILTGGEKvg----dkGYFIRPTVFYDvnedMRIVKEEI 405
gi 50551783  343 ESVkvgdp-fdpnsLQGPQQNKNQFKKILGYIEQgq-keGAHLLCGGSAqag--pnkGYFIQPTVFTDvnndMSIVREEI 418
gi 66805929  333 ESRvlgdp-lskdtHQGPQVNKFQFESILRYIETgk-reGATLVCGGKRfg----nkGYYIEPTVFSNvtdvMTIAREEI 406
gi 119175659 324 TKAvagdp-llsntTKGPIVSADQHSKIMGYIKKgq-deGARLLHGGGQp------aPGYIENTAFVDvnedMTIMREEI 395
gi 46122907  321 RDAvmgdp-llddtVKGPVISSTQKNRIMEYITKak-seGIELLHGSAEpd----skGNFVPNTAFINvsptATIMREEV 394
gi 2506349   349 ESIkvgdp-fdestFQGAQTSQMQLNKILKYVDIgk-neGATLITGGERlg----skGYFIKPTVFGDvkedMRIVKEEI 422
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #                           #                                     #             
2O2Q_A       421 FGPIMIISRFAdgdVDAVLSRANATEFGLASGVFTRdINKALYVSDkLQAGTVFINTYnkTDVAAPFGGFKQSGFGKDLG 500
gi 66803593  393 FGPVVVILPFKt--VDEVIKRANDTTYGLAAGVWTKdISLALNVSNkLKSGSVWVNGFniLKSQIPFGGFKQSGFGRDLS 470
gi 118361802 392 FGPVLVVLKPWk-tFDEVIKRANDTNYGLAAYAVTQnAGNVEKFVReVKAGSFYINQGalSAHQMPFGGYKESGFGKDNG 470
gi 150866913 403 FGPVVVVGKFSs--DEEVITYANQTQYGLGAAIFTKdITVAHTMAAeIQAGMVWINSSndSDVHIPFGGVKMSGVGRELG 480
gi 20981689  406 FGPVVTVAKFKt--LEEGVEMANSSEFGLGSGIETEsLSTGLKVAKmLKAGTVWINTYndFDSRVPFGGVKQSGYGREMG 483
gi 50551783  419 FGPVLTITKFNt--VDEVIDMANDSEYGLAAGIHTTdINKAHYVAEnIASGTIWVNCYnaFHEAVPFGGYKQSGFGKEMG 496
gi 66805929  407 FGPVMSILRFEt--VQEAIDRANDSEFGLVGAVFTKdINKSIIVSDqVQSGLVWVNSFniIDPSIPWGGFKSSGKGRDAS 484
gi 119175659 396 FGPVAAISKFKt--ESEAIQKANNTEYGLSSAIFTNnVSRADRVSRaLESGQVTVNSWgmVHANVPFGGIKMSGFGKDMG 473
gi 46122907  395 FGPVASFAKFKt--EEEVISLANDNEYGLAAAVFTNdISRAVRVSDqIEVGIVFVNTWgsISANSPFGGIKQSGFGRENG 472
gi 2506349   423 FGPVVTVTKFKs--ADEVINMANDSEYGLAAGIHTSnINTALKVADrVNAGTVWINTYndFHHAVPFGGFNASGLGREMS 500
                        490
                 ....*....|....*
Feature 1                       
2O2Q_A       501 EAALNEYLRIKTVTF 515
gi 66803593  471 EYAIQGYLSVKAVTI 485
gi 118361802 471 EEGLLEYTQLKSVYY 485
gi 150866913 481 EYGLSIYTQAKAIHV 495
gi 20981689  484 EEVYHAYTEVKAVRI 498
gi 50551783  497 RDGLENYLQTKAVRV 511
gi 66805929  485 EYCLSVWTETKTTVL 499
gi 119175659 474 EESLDGWTGTKTIKY 488
gi 46122907  473 TDALNDWTQVKCVKI 487
gi 2506349   501 VDALQNYLQVKAVRA 515

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