2D4E


Conserved Protein Domain Family
ALDH_F8_HMSADH

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cd07093: ALDH_F8_HMSADH 
Click on image for an interactive view with Cn3D
Human aldehyde dehydrogenase family 8 member A1-like
In humans, the aldehyde dehydrogenase family 8 member A1 (ALDH8A1) protein functions to convert 9-cis-retinal to 9-cis-retinoic acid and has a preference for NAD+. Also included in this CD is the 2-hydroxymuconic semialdehyde dehydrogenase (HMSADH) which catalyzes the conversion of 2-hydroxymuconic semialdehyde to 4-oxalocrotonate, a step in the meta cleavage pathway of aromatic hydrocarbons in bacteria. Such HMSADHs seen here are: XylG of the TOL plasmid pWW0 of Pseudomonas putida, TomC of Burkholderia cepacia G4, and AphC of Comamonas testosterone.
Statistics
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PSSM-Id: 143412
View PSSM: cd07093
Aligned: 37 rows
Threshold Bit Score: 586.454
Threshold Setting Gi: 87200623
Created: 14-Dec-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
NAD bindingcatalytic
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:
  • Structure:2D4E: Thermus thermophilus 5-carboxy-2-hydroxymuconate semialdehyde dehydrogenase Hb8 with bound NAD cofactor, defined using 3.5 A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2D4E_C        46 SLDPATNEVLGVAARggerEVDRAAKAAHEAFQRws--rtKAKERKRYLLRIAELIEKHADELAVMECLDAGQvl-rIVR 122
gi 84502146   33 VTDPATGREVAILREsdadQVDRAVAAARAAFPAws--rtTVADRQRALMAIHDAILANADELARLETLNTGIplgqTRT 110
gi 89056266   31 IVNPATGGPAGTLQEadaaEVAEAVRAARTAFDKtdwprwSVPRRQALLLAIRDKIVEHADKLAHLECIALGVplkeLKA 110
gi 119503017  39 VHFPGTGESIASLQEddatQVIAAVNVARERFRSgdwskcTTTEKQSVFRAAAQAIRNNAHELALQESLCAGLpfahLAS 118
gi 108803876  34 DISPITNEPIAEVSRageaEVDAAVRAARDAFPGwa--alGPAGRAPYLHRLADLIDANVERLAAVECMDMAMllrsLRA 111
gi 87200623   32 RCPFDWSWKLADISRgtvtTAAQAAQAATDAFPAwa--alSAAERGAYLHRLADLIEANVEKLAMIECLDMGMllesLRL 109
gi 154252237  32 NFSPIDGSHLGDLSAggkaEADAAVAAARKAFPAwa--alGPKGRLPILKKFAEGIKARVNDLAAVETMDNGSllmgNVH 109
gi 757827     21 TTNPATGEVLADVASggeaEINQAVAAAKEAFPKwa--nlPMKERARLMRRLGDLIDQNVPEIAAMETADTGLpihqTKN 98
gi 33599726   20 TINPATQETLAEVAAggeaEVHAAVAAAKAAFPKwa--glPAAERARLVRKLGDLIAAHVPEISRTETNDCGQtiaqTGK 97
gi 46201399   19 NLNPATNEVICQIAAggeaEVARAVAAAKEAFPKwa--glAPAQRAKLLRKVGDLINQHVDEIAKLESLDTGQsywrTKK 96
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                       ###     #                 # ##   
2D4E_C       123 AQVARAAENFAFYAEYaehameDRTFPvdrdWLYYTVRVPAGPVGIITPWNAPLMLSTWRIAPALAFGNTVVLKPAEwsP 202
gi 84502146  111 MHIPRAAQNFRFFAEFinq-dnGEVFTqepgYMSLVTHEPMGVCALIGPWNMPLGLTAMKMAGALAFGNTCVVKPSEitP 189
gi 89056266  111 RHMLRAAENFRFFAEYigq-arGDLYDqtpgYRTIVTREPVGVAALIAPWNAPVALASMKLAAALAFGNTCVLKPSEqtP 189
gi 119503017 119 RQIPRAAENFDFFADYigq-laGETYDqlhdYRTVVTRQPAGVAAIFTPWNACLALSSMQIASCIAFGNSCVLKPSEfaP 197
gi 108803876 112 RVIRRGARNYRAYADLaas-yeERDWRsq-gTWNRVQRMPAGPAAIITPWNAPFMLSTWKTAPALAAGCTVVLKPAEwsP 189
gi 87200623  110 RVILRGAANFRNYADLat--ghEERVWssrgTLNRVIRMPAGPALIITPWNAPFMLSTWKCAPALAAGNTVILKPADwsP 187
gi 154252237 110 RVVPRAAQNIEFFADWalt-ldGHSIDsp-eVVNHVRYDPAGVAVLITPWNAPLMLTTWKVGPALAAGNTVVVKPPEwaP 187
gi 757827     99 VLIPRASHNFEFFAEVcqq-mnGKTYPvvdkMLNYTLVQPVGVCALVSPWNVPFMTATWKVAPCLALGNTAVLKMSElsP 177
gi 33599726   98 QLVPRAADNFYYFAEMctr-vdGHTYPtp-tHLNYTLYHPVGVCALISPWNVPFMTATWKVAPCLAFGNTAVLKMSElsP 175
gi 46201399   97 MLVPRAADNFYFFADTcqh-vdGETYPtn-dHLNYTIYQPVGVVGLISPWNVPFMTATWKTAPCLAFGNTAVLKMSElsP 174
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                   #   ##             ###  #  #              ##         
2D4E_C       203 FTATKLAEILKe-aDLPPGVFNLVQGFGeeaGAALVAHPlVPLLTLTGETETGKIVMRNAAdhLKRLSPELGGKSPALVF 281
gi 84502146  190 LTLARLFEILAasgALPEGVINLVNGRGhvtGTALSGHPdIDMVSFTGGTETGARILTALArgIKGGAMELGGKSPSIVF 269
gi 89056266  190 FALHLLVRLLHd-aGLPDGVVNLVNGRGdvtGAALAAQDgVDLISFTGGTTTGRTIMAEAGrrLVPCTMELGGKSANIVF 268
gi 119503017 198 LAVLQLVRLLEs-vGLPTGVVNVVNGRGsvtGAALATAAgVDRISLTGRGDTARKVMAAAAaqLTPVHFELEGNSANIIF 276
gi 108803876 190 LSCSLLADLVAe-aGFPPGVFNVVQGIGeeaGAALVAHPlLRRVSFTGSPETGRSIGVAAAknLVPFTAELGGKGPLIVF 268
gi 87200623  188 LSASILADLIEe-aGFPAGVFNIVQGLGaelGNALTSDPrIKRISFTGSVPTARVIGKAAAenIVPLTAELGGKSPLLVF 266
gi 154252237 188 LTCSLMADIAHa-aGVPAGVLNVVQGIGeeaGDALVNHPdVDRISFTGSTDTAKIIGQAAArsITPMSAELGGKSPFIVC 266
gi 757827    178 LTADRLGELALe-aGIPAGVLNVVQGYGataGDALVRHHdVRAVSFTGGTATGRNIMKNAG--LKKYSMELGGKSPVLIF 254
gi 33599726  176 LTAARLGELALe-aGIPPGVLNLVHGYGrdaGEPLVAHPdVRAVSFTGSTATGNRIVQAAG--LKKFSMELGGKSPFVIF 252
gi 46201399  175 LSADRLGQLILe-aGIPAGVFNIVHGYGsavGEALVKHPdVRGVSFTGSTATGNRIIQSGG--LKKYSMELGGKSPNIVF 251
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                               #                                                        
2D4E_C       282 ADaDLERALDAVVFQIFSFnGERCTASSRLLVEekIFEDFVGKVVERARAIrvGHPLDpeteVGPLIHPEHLQRVLGYVE 361
gi 84502146  270 DDaDLDRAIDGAMLGSFMNnGQMCLAGSRLFVQrgVADRFIEAFTDRTRALriGDPFDpateVGPMINEAQLKRVERFVA 349
gi 89056266  269 ASaDRDRALDAALASIYSNnGQQCLAGSRIFVEraIADDFISDFVARARSIriGSPTDdsteLGPLATAAHRDRVLEFAR 348
gi 119503017 277 DDaDIDRAIDGALVSAFGNsGQICIAGSRILVQqnIADRFIDAFVARTKNLqvGDPLDnrteVGPMAFAQHQQKVLNYFA 356
gi 108803876 269 EDaDLDAAARRAAGQYDDA-GQVCLAGTRLLVQesVAEEFLELFHRYADEHvlGDPRSehttVSPLIHPDHLRRVEGFVE 347
gi 87200623  267 ADaDIDAAAKKAAGQYDDS-GQVCMAGTRIIVEesVKDEFLAKFHAYADAHvmGDSRDaattMSALIHPVHVERVLGFIE 345
gi 154252237 267 ADaDLDAAAQTVAGQYMNA-GQVCLAGTRIMVEkkIEEEFLEKVRGAASHMivGDPRDkdtrVGPLIHKEHFARVAGFVE 345
gi 757827    255 EDaDIERALDAALFTIFSInGERCTAGSRIFIQqsIYPEFVKRFAERANRLrvGDPNDpntqVGALISQQHWEKVSGYIR 334
gi 33599726  253 DDaDLDRALDAAIFMIFSNnGQRCTAGSRILVQqsIYADFTQKFVERARRIrvGDPLDegtiVGPLISPAHLAKVRGYIE 332
gi 46201399  252 DDcDFERAVDAAIVAVYGNnGESCTNGTRILVQdgIYDRFVAALAERTKKVvvGDPLDeatnVGPMITRDHWKKVTGYIE 331
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                          # #                           
2D4E_C       362 AgkreGARLLVGGERaktsfr--gedlsrGNYLLPTVFVGen-hmKIAQEEIFGPVLVAIPFKdEEEALRKANDTKYGLA 438
gi 84502146  350 SgledGAELIAGGARhea--------lpdGFYIQPTAMIAqsnalAICQEEIFAPFATIQIFDdEEEVVGRANDSIFGLV 421
gi 89056266  349 TapqdGVEILTGGGVpgdlr----ddisgGHYIEPIAAFApgnasVVCQQEIFGPFATFQIFDsYNQVISAANNTEFGLV 424
gi 119503017 357 LaentDATLLTGGRAmpe--------lggGYFVAPTVFKVldnnsPLCQEEIFGPVVTIQIFNeDEEAWTIANDSPFGLI 428
gi 108803876 348 RarreGHRVLRGGRRsp----------agPLFYQPTLIEPlsndaEIVQREVFGPVLTLQTFAdEEEAVSLANSTEYGLS 417
gi 87200623  346 RaraaGDAIVRGGRLwk----------egSNWIEPTLIEPknnqsEVVQSEVFGPVLTFQTFRdEAEGVALANSTAYGLS 415
gi 154252237 346 RaradGAVPLWGGNRan----------fgELYFEPTLFANitpelEIAQREVFGPVLTWQNFSsDDEVIELANNTRYGLA 415
gi 757827    335 LgieeGATLLAGGPDkpsdlp---ahlkgGNFLRPTVLADvdnrmRVAQEEIFGPVACLLPFKdEAEGLRLANDVEYGLA 411
gi 33599726  333 LgskeGATMLCGGLDrpsyapeldarvrdGNFVWPTVFADvdnrmKIAQDEIFGPVACLIPFRdEAHAIELANDIEYGLS 412
gi 46201399  332 LgikeGARVVAGGLGepeglp---ahlknGNFVKPTVLADvdnswRVAQEEIFGPVACVIRFKdEAEALKIANATTYGLA 408
                        410       420       430       440       450       460
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
Feature 1                                            #                            
2D4E_C       439 AYVFTRdLERAHRLALeLEAGMVYLNSHnVRHLPTPFGGVKGSGDRREGGTYALDFYTDLKTIAL 503
gi 84502146  422 GYVWTRdLERAFRLSRgLQTGTVLVNTPiMRDLRTGFGGIRQSGIGREGAKGSRAMFTEMKSTIV 486
gi 89056266  425 SYVWSDhLPTVERAVRdLRSGVVWVNTTmTRELRAPFGGFKSSGVGREGGEACERFYTEPKTVTV 489
gi 119503017 429 GYCWTEsLHRAQLFQQrLEAGTLWLNTPiARDLRAPFGGFKASGIGRDGPRHAADFFTEEKATIA 493
gi 108803876 418 GIVYTSsMGRADRVGRaVRAGVVWVNTFlVRDLTAPFGGMGISGIGREGGDYALDFYSDLKTLQI 482
gi 87200623  416 GIVYTGsAERAERVGRqVRGGTVWVNTFlVRDLTAPFGGIGISGIGREGGDYALDFHSDLKTLQI 480
gi 154252237 416 GTLFSRnEKRAMDIASrVTAGTLWVNCFfVRDLAAPFGGAKDSGIGREGGTWSFDFYTDIKNISV 480
gi 757827    412 SYIWTQdVSKVLRLARgIEAGMVFVNTQnVRDLRQPFGGVKASGTGREGGEYSFEVFAEMKNVCI 476
gi 33599726  413 SYVWTEsVGRAHRVAAaIEAGMCFVNSQnVRDLRQPFGGTKASGTGREGGTWSYEVFCEPKNVAV 477
gi 46201399  409 SYVWTEnGAKAIRMAEgIEAGLVFVNSQnVRDLRQPFGGIKGSGTGREGGHYSYEAFLEVKNVCV 473

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