Conserved Protein Domain Family
ALDH_KGSADH-YcbD

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cd07097: ALDH_KGSADH-YcbD 
Bacillus subtilis NADP+-dependent alpha-ketoglutaric semialdehyde dehydrogenase ycbD-like
Kinetic studies of the Bacillus subtilis ALDH-like ycbD protein, which is involved in d-glucarate/d-galactarate utilization, reveal that it is a NADP+-dependent, alpha-ketoglutaric semialdehyde dehydrogenase (KGSADH). KGSADHs (EC 1.2.1.26) catalyze the NAD(P)+-dependent conversion of KGSA to alpha-ketoglutarate. Interestingly, the NADP+-dependent, tetrameric, 2,5-dioxopentanoate dehydrogenase (EC=1.2.1.26), an enzyme involved in the catabolic pathway for D-arabinose in Sulfolobus solfataricus, also clusters in this group. This CD shows a distant phylogenetic relationship to the Azospirillum brasilense KGSADH-II (-III) group.
Statistics
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PSSM-Id: 143415
View PSSM: cd07097
Aligned: 21 rows
Threshold Bit Score: 690.142
Threshold Setting Gi: 33598663
Created: 30-Dec-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:catalytic residues [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 1169281    10 YLNFINGEWVKSQSgdmvkvENPADVNDIVGYVQnstaeDVERAVTAANEAKTAWRklTGAERGQYLYKTADIMEQRLEE 89
gi 56965568    5 YLNYIDGEWKPSISgnvfygRNPANYEEILGAYQastaeDARLAIKSAAAAFPNWKnkSAIERADVLYQLMPLLAAEKEK 84
gi 108803393   8 SRNYIGGEWVPADSgeveevRNPARPSEVLGTAPrsdrgEMERAVAAAQEALPGWRsaLPAARGQILLRAADIIDSRSEE 87
gi 70607429    2 EKGVYIGKLVKPVDrewyevRNPADYREIVSKFPrlhrkDVVDAIKIAKESYEKWReyTAYERAKILFKTADILESRMQE 81
gi 55379616    5 YENYIDGEWTGSGEtq--evTNPADETDVVSTVPvasaaDADEAVAAAAAATDEWGemPGPERGAILRETGEILKSRKDE 82
gi 116620096   2 FANYINGEWIAAGAtf--enRNPANTDEVVAVMAkgsaaDIAAAADAAGAAFPAWSamSGPARGNILYKAADILDKTFDS 79
gi 159042340   3 ERGVFIGKLIIPRDrefyeiRNPADTRQVVAKFPrlrrdDAREAIGVAKEAFAKWGktLPVERARILYKVADIIESRADE 82
gi 74556534    8 ADKWIKGSGEEYLD------INPADKDHVLAKIRlytkdDVKEAINKAVAKFDEWSrtPAPKRGSILLKAGELMEQEAQE 81
gi 33597371    4 ITSLIDGQWAGGAGds--enINPSDLSAPVCLVAqasaqQAAQAVEAARRAQPGWAatGLAARAQILKDIGRGIVANSQQ 81
gi 33600202    5 YRSFVAGQWQDGGEpv--alYNPSDLQEEAGVYMhapveMVNQAVRAAEEALDAWRglHAMARSRLLDGIADGLVRRRDE 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                           #            
gi 1169281    90 IAACATREMGKTLpEAKGETARGIAILRYYAGEGMRKTGDVIPStDKDALMFTTRVPLGVVGVISPWNFPVAIPIWKMAP 169
gi 56965568   85 LAAIITKEVGKTMaAARKEVDASIQALKHFSGAANRLAGETVPAgNPETFAYTIKEPLGPVGVITPFNFPLGIGIYKIAP 164
gi 108803393  88 LARLMALEAGKPLgESRAEVSRAAAIFRYYGSEGWRLYGVEAPStRPGVRITSTREPLGVVGLITPWNFPLAIPSWKMAP 167
gi 70607429   82 IAKTLTMEEGKTLgESAYEVERVTSLLRYYGGLTLNSHGKTLQSsMKNSMHLTVREPIGVVGIITPWNFPFLIPGWKIAP 161
gi 55379616   83 LAETLTREEGKPLgEAEGEVQRAIDIFYYYAEKARDFGGTVKQPsGGRAGLQTKKEPMGVAALITPWNYPIAIPAWKIAP 162
gi 116620096  80 VAADMTREEGKTLpEAKGEVRRAINILRYFAGEGSRLPGMLVPSeRDRVHMFALRKPVGVVGLITPWNFPSAIPAWKLAP 159
gi 159042340  83 MARTLTLEEGKTLpDSMFEVVRTVNLLRFYAGLITRGQGKVISSqDRNTIIMTTREPLGVISVITPWNFPLSLPAWKIIP 162
gi 74556534   82 FALLMTLEEGKTLkDSMFEVTRSYNLLKFYGALAFKISGKTLPSaDPNTRIFTVKEPLGVVALITPWNFPLSIPVWKLAP 161
gi 33597371   82 LAHILASEQGKTLpEARGEVQRAADIFDYYAGEVFRLGGEMFPSiRAGVEVEISREPLGVVALITPWNFPIAIPAWKTAP 161
gi 33600202   83 LAMAIAREQGKPLrDALAEATRAADIFRYFASETIRQKGYTYASiRPGVRVEVQRNPVGVVALVTPWNFPVVIPAWKAAA 162
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 1169281   170 ALVYGNTVVIKPATETAVTCAKIIACFEeaGLPAGVINLVTGPGSvVGQGLAEHdgVNAVTFTGSNQVGKIIGQAALa-- 247
gi 56965568  165 ALIAGNTVVYKPHNDTVQIAARLVELFIeaGTPKGVLNLVTGDGDkVGQELGENrtLKAISFTGSSDVGLRLGRSVTn-- 242
gi 108803393 168 ALICGNTVVIKPAANAVLNAAELVRILEeaGLPGGVVNLVMGPGGsSGDALVRDerVRAISFTGSTAVGLSIQERAA--- 244
gi 70607429  162 ALATGNTVVFKPASNTPTVAFELVNALYeaGLPEGVVNLVTGSGReVGDEIVTNkeIKAVSFTGSLEVGREVNSKIGn-- 239
gi 55379616  163 ALAVGNTVVIKPAMQAPTVGAMIVEALDeaGIPDGAINLVCGPGSeVGEQLTTHddVDVVSFTGSASVGEHVYEQATs-- 240
gi 116620096 160 ALICGNTVVIKPASAAPLSAWRIVEALHqaGIPKGVVNFIAGSGGeLGQALVNAapLKAVSFTGSCEIGDWLHAEASk-- 237
gi 159042340 163 AIATGNTVVWKPASITPTIAYELVKAFYdaGLPEGVLNLVTGSASeVGDELVTNkdVDAITFTGSLQTGKEINEKVGrmn 242
gi 74556534  162 ALAAGNTAVIKPATKTPLMVAKLVEVLSkaGLPEGVVNLVVGKGSeVGDTIVSDdnIAAVSFTGSTEVGKRIYKLVGnkn 241
gi 33597371  162 ALACGNAVVLKPSEFTPATAVMLAEIIRaaGVPDGVFNLVLGTGAdVGQVLTGHpaVAGVSFTGSSATGRKIAASAVa-- 239
gi 33600202  163 ALAYGNTVVMKPSEIAPCGAWILADVMReaGLPAGVFNLVLGAGQ-AGAALVAHeaVAAVSFTGSVATGKRIAAAAA--- 238
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #                              #  #                                      
gi 1169281   248 rGAKYQLEMGGKNPVIVADDADLEAAAEAVITGAFRSTGQKCTATSRVIVQsgIYERFKEKLLQRTKDItIGDSLKEdVW 327
gi 56965568  243 rGGKMQAEMGGKNATVILEDADLDKAVSGIVISGLLNNGQSCTGTSRVIVPknIAKTVTSKLVEKATAIrVGDGFNEnVE 322
gi 108803393 245 -GKKVQLEMGGKNPYVVMEDADLEDAAAKVAYGAFGYAGQKCTASSRAVVVeeVYEAFLEQVVEATRALkLGEPLDEeAV 323
gi 70607429  240 rFTRLQLELGGKNATVLTKNGDFNLAIEHVTWSVMVNTGQVCLATSRFLVPkeLHDKAVNAMTESFKSLvVGNALKQpVH 319
gi 55379616  241 nGKRAQAEMGGKNPTVVMPSADVDKAADIVGAGAFGGTGQACTATSRAIVHedVYDEFLDAIVDYAESLeIGDGLDR-AG 319
gi 116620096 238 rRLRIQLEMGGKNPTIVLADADFGSAVENVVNAAFFSTGQKCTATSRAIVEdaIYDKFVAAVVARTRKLkVGDGMQPgID 317
gi 159042340 243 rFIRVQLELGGKNATVLSKNGDINLAVELTARSAFGLTGQACTATSRFLVPedMHDEVLSKLIERTKKIvVGNGLKSgVD 322
gi 74556534  242 rMTRIQLELGGKNALYVDKSADLTLAAELAVRGGFGLTGQSCTATSRLIINkdVYTQFKQRLLERVKKWrVGPGTED-VD 320
gi 33597371  240 gHKKLQMEMGGKNPLVVLDDADLEIALACALDGAFHATGQRCTASSRLIVTpgIHDRFVQELAQRVMALnVGHALDErSQ 319
gi 33600202  239 -GKRIQLEMGGKNPLIVLDDADLDTAVACAIDGAFLYAGQRCTASSRIIVTegIHAAFVKRLLAQTASLaVGHALDEaTV 317
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 1169281   328 MGPIASKNQLDNCLSYIEKgkqeGASLLIGGEKLengk-yqnGYYVQPAIFDNVtseMTIAQEEIFGPVIALIKVDsIEE 406
gi 56965568  323 NGPVANEAQLQKYLHYVDIavkeGAKLECGGKRLfgeg-kdkGYFVAPTVLSGVtpeMTIAQEEVFAPVVAIMEVDsFDE 401
gi 108803393 324 IGPVINESQYESILGAIEQaredGRVVLDGGATGpr----eeGYYISPVIVADVdngSKTAREEIFGPVLAVIKARdFDH 399
gi 70607429  320 MGPLSSRDQYEKVLGYVEIgkdeGAKLVYGGEPIkged-ydyGYFVKPTIFDNVtkdMRIAKEEIFGPVLAIMTYEsLDE 398
gi 55379616  320 MGPHVSEDELAGSLEYIDVaqseGATLETGGEELtsge-ydgGNFISPAVFSDVepdMRIAQEEVFGPVLAVMSVSdFDE 398
gi 116620096 318 IGPCVDQSQMETVLRYIEIgrkeCGEPACGGKRLtega-lakGYFVEPTVFAGVtpdQTIAREEIFGPVLAIMRARdFED 396
gi 159042340 323 MGPLASKEQYDKILSYIEIgrneGAKLVYGGQPIkgseefdhGYFVMPTIFDGVtpdMRIAKEEIFGPVLAVMTYRtLDE 402
gi 74556534  321 MGPVVDEGQFKKDLEYIEYgknvGAKLIYGGNIIpg-----kGYFLEPTIFEGVtsdMRLFKEEIFGPVLSVTEAKdLDE 395
gi 33597371  320 IGPVSNGAQFDKDLSYIALar-dEAQRVIGGAALqra---qrGYYLQPCLALDVsldARIAREEVFGPLACVLRAQdYEH 395
gi 33600202  318 IGPVVNAEQFEKNRRYADLar-qEGGTVAGGRALsrp---trGHYFEPCLVTGLdnsATVCREEVFGPFACVLTARdYEH 393
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 1169281   407 ALNIANDVKFGLSASIFTEnIGRMLSFIDeIDAGLVRINAeSAGVELQAPFGGMKQSs-SHSREQGEAAKDFFTAIKTVF 485
gi 56965568  402 AIAVANQTDFGLSSAIYTTnLQKAHEFVRrVETGVTHINIpSNHYENQLPFGGKKNSs-IGPREQGSTALDFWVDTKAVY 480
gi 108803393 400 AVEIANDTRYGLTAGIATRsLRYAEEFIErSQTGLVNVNLpTAGVEFQVPFGGSKQSg-VGGREQGMAAIEFYTGWKTAS 478
gi 70607429  399 AIEIVNSTDYGLTAEIVTNnLNEAMKFSEgVEAGVVKVNRpTVELDQWVPYGGFKGSgnDVYKELGEEALDFYTKIKAVH 478
gi 55379616  399 GVEVANDIDYGLSASIVTDrIKEENEFIErSESGVVKVNEkTTGLELHVPFGGLKRSstNTYREQGDAGLEFFSYIKTVY 478
gi 116620096 397 AMSIANSIPFGLSSSIQTTnLSRAFEYIYrAEAGLLTVNLpSAGVEYQLPFGGTKDSs-FGPKEQGPAALEFYSDYKTVY 475
gi 159042340 403 AIDIVNSTEYGLIAGIVTRdISEAAKFTEgVKVGVVKVNKpTIGLEPWVPYGGVKGSgnDMYKEMGEEAVEFFTRIKAIY 482
gi 74556534  396 AIRLVNAVDYGHTAGIVASdIKAINEFVSrVEAGVIKVNKpTVGLELQAPFGGFKNSgaTTWKEMGEDALEFYLKEKTVY 475
gi 33597371  396 ALAIANDTEYGLSAGICTQsLKHATHFRRhVQSGLAMVNMpTSGVDFHVPFGGRGASs-YGSSEQGHYAREFFTSLKTSY 474
gi 33600202  394 ALAIANDTPFGLSSGLCTTsMKHAQHFKAhTKSGLASINTsTSGVDFHVPFGGNGASs-YGPREQGEFAIEFFTTLRTVY 472

                 .
Feature 1         
gi 1169281   486 V 486
gi 56965568  481 L 481
gi 108803393 479 V 479
gi 70607429  479 L 479
gi 55379616  479 R 479
gi 116620096 476 L 476
gi 159042340 483 V 483
gi 74556534  476 E 476
gi 33597371  475 I 475
gi 33600202  473 I 473

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