1CW3,1BXS


Conserved Protein Domain Family
ALDH_F1AB_F2_RALDH1

?
cd07141: ALDH_F1AB_F2_RALDH1 
Click on image for an interactive view with Cn3D
NAD+-dependent retinal dehydrogenase 1, ALDH families 1A, 1B, and 2-like
NAD+-dependent retinal dehydrogenase 1 (RALDH 1, ALDH1, EC=1.2.1.36) also known as aldehyde dehydrogenase family 1 member A1 (ALDH1A1) in humans, is a homotetrameric, cytosolic enzyme that catalyzes the oxidation of retinaldehyde to retinoic acid. Human ALDH1B1 and ALDH2 are also in this cluster; both are mitochrondrial homotetramers which play important roles in acetaldehyde oxidation; ALDH1B1 in response to UV light exposure and ALDH2 during ethanol metabolism.
Statistics
?
PSSM-Id: 143459
View PSSM: cd07141
Aligned: 18 rows
Threshold Bit Score: 863.966
Threshold Setting Gi: 114051966
Created: 2-Jan-2009
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:
  • Structure:1CW3; Human mitochondrial aldehyde dehydrogenase (ALDH2) complexed with NAD+ and Mn2+, defined using 3.5 A.
    View structure with Cn3D
  • Structure:1BXS; Sheep liver cytosolic aldehyde dehydrogenase (ALDH1) complexed with NAD+, defined using 3.5 A.
    View structure with Cn3D
  • Citation:PMID 9195888
  • Citation:PMID 9862807

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1CW3_A         8 QPEVFCNQIFINNEWHDAVSRKTFpTVNPSTGEVICQVAEGDKeDVDKAVKAARAAFQLGSPWRrMDASHRGRLLNRLAD 87
gi 37958173    3 QVEVKYTQIFINNEWHDSISGKTFeTINPFTEEKLANVQEGDKaDIDRAVVAAVDAFRFDSPWRqMDASQRGHLLYRLAD 82
gi 114051966   3 KVDVKYTKLFINNEWVDAVSKKTFpTINPQDETVITQVAEGDKaDIDLAVAAAKKAFHRYSPWRtMDASQRGLLLLKLAE 82
gi 115899350  39 NPDIQHHKLFINNEWVNSVSGKTFpTINPATEEVIAEVAEADKaDVELAHAAASEAFRLGSPWRtTNASRRGALINKLAD 118
gi 198424229   9 KPKIQYTQIFINNEWRNSISGKTFaTINPCTEEKICDIQEGDKaDVDLAVKAASEAFKLGSPWRtMDASQRGVLLNKLAD 88
gi 8131883     7 NPEIRYKQLFINNEWVNAASGKTFpVINPATGKKIVDIQEGDKaDVDKAVAAAKRAFEPGSVWRnTDASDRGRLLNKLAD 86
gi 156396842  10 NPQIKYTKLFINNEFVDCTSGKVFpTINPTTGEKICDISEGDKeDVDKAVKAAKEAFKLGSAWRtMDASMRGKLLYKLAQ 89
gi 196001343   8 NPEIKYTKIFINNEWHDSVSGKTFpTFNPATGEKICDIAEGDKaDIDLAVAAAKQAFKKGSTWRtMDASKRGRLISKLAE 87
gi 2494070    12 DLKVEFTKIFINNEFVDSVSGKTFaTINPSTGEKLAEVQEGDKaDIDKAVAAARAAFKRDAEYRkHDASDRGRLLFKLAD 91
gi 156551115  10 NPEIKYTKLFINNEWVDSVSGKKFtTINPANEKVIAEVSEAEKaDVDKAVKVARAAFERGSVWRnLDASARGRLIYNLAQ 89
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                               ####     
1CW3_A        88 LIERDRTYLAALETLDNGKpyviSYLVDLDMVLKCLRYYAGWADKYhGKTIPiDGDFFSYTRHEPVGVCGQIIPWNFPLL 167
gi 37958173   83 LIERDQDYIASLESMDNGKpk-tMALFDVDLAIKVFRYYAGYADKIhGKTIPaDGKVFAFTRIEPVGICGQIVPWNFPFL 161
gi 114051966  83 LLESQSRYLAELETLDCGKpv-kQSEEEVYFSASVLRYYAGKADKIlGNTIPsDGEVLTFTMKEPVGVCGQIIPWNYPIP 161
gi 115899350 119 LVERDWAHLASLETLDNGKpyqdSYTIDVPGVLATLRYFAGWADKIeGKTIPvDGDFMTYTRHEPVGVCGQIIPWNFPLL 198
gi 198424229  89 LINRDRHILASLETLDNGKpynvSYNVDLELVIRCYRYYAGFADKNhGKLIPmDGNAHAYTRHEPVGVCGQIIPWNFPLL 168
gi 8131883    87 LIERDRQYIASLESLDNGKpfsdSFNVDMVLVCKCLRYYAGWTDKIcGKTIPmDGNFFCYTKLEPVGVCGQIIPWNFPLL 166
gi 156396842  90 LIDRDIAYLASLETIDSGKlf-sDSVGDMQSSANCFRYYAGWADKVtGKTIPaDGPYFVYTRHEPVGLVGAITPWNFPLN 168
gi 196001343  88 LMERDQQYLASLESLDNGKpvrvANFADLPGAMLCFRYYAGWADKIhGKTIPvDGPYMTYTRHEPIGICGQIIPWNFPLL 167
gi 2494070    92 LIEAHRVQLRTLETLDNGKpfamSYLGDTLMAQKVLRYYAGFADKIvGQTIPaDGNVFCYTRHEPVGVVGAITPWNFPLH 171
gi 156551115  90 LIEDHVNEIASLESLDNGKpf-nNSYYQTLESTNVLRYFAGCADKIhGLTIPsDGKEFALTRKEPVGVVGAIIPWNYPII 168
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                          #  #                             #   ##            #  #  #    
1CW3_A       168 MQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGpTAGAAIASHEDVDKVAFTGSTEIGRVI 247
gi 37958173  162 MASWKFGPALCAGNTVVLKPAEQTPLSALYLASLTKEGGFPPGVVNVVPGFGeTAGAALVDNPKVDKIAFTGSTEIGKLI 241
gi 114051966 162 MMSWKIAPALAAGCTVVLKPAEQTPLTALAVAALVKEAGFPPGVVNVVPGYGaTAGGALTHHPDVDKIAFTGSTEVGRII 241
gi 115899350 199 MLAWKWAPALACGNTIIMKTAEQTPLTALHMAELAKEAGFPPGVINVLSGYGpTAGAAIASHPGIDKVAFTGSTEIGRII 278
gi 198424229 169 MQAWKLGPALSMGNVVVMKPAEQTPLTALYVAALIKEAGFPPGVVNMIPGYGpTAGAAISEHMDVDKVAFTGSTEVGHLI 248
gi 8131883   167 MLAWKIGPAIACGNVVVLKPAEQTPLTALYTAALTKEAGFPPGVVNMIPGYGpTAGAAISEHYDVDKVAFTGSTEVGQIV 246
gi 156396842 169 MASVKIAPALACGNVVILKPAEQTPLTALYFCALVKEAGFPAGVVNVIPGYGpTAGAAITTHLDIDKVSFTGSTEVGRLI 248
gi 196001343 168 MASWKLAPALACGNVVVLKPAEQTPLSVLYLCQLAVEAGFPPGVINVVPGYGpTAGAAISNHPDIDKVAFTGSTEVGQII 247
gi 2494070   172 LAASKIAPAIAAGCTLVLKPAEQTPLTALYLASLVKQAGFPAGVINIVPGLGhTAGAALTNHPDINKITFTGSTEVGQLI 251
gi 156551115 169 LLSFKLGMALAAGCTVVVKPAEQTPLTALYVASLVKEAGFPPGVVNVLPGYGpSTGAAISSHPDIDKVSFTGSSVVGKAI 248
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                       ##                                #                              
1CW3_A       248 QVAAGssNLKRVTLELGGKSPNIIMSDa-DMDWAVEQAHFALFFNqGQCCCAGSRTFVQedIYDEFVERSVARAKSRvvG 326
gi 37958173  242 MRNGSh-SMKRITLELGGKSPLVVTENveDIAQAARTAQDSCFLNmGQCCCAGTRTFVHesIYDEFVKHSVEYCQSHvfG 320
gi 114051966 242 LGAASavNLKRVTLELGGKSPLVVFNDa-DVEKAAQIAHAAAFANaGQCCCAGTRTYVQsgIYDKFVKKAAEIAEKRsvG 320
gi 115899350 279 AQSAGasNLKKVTLELGGKSPNIILADa-DLDHAVEQSHSAIFWNmGQACCAGSRTFVEdkIYDEFVERSVERAKSKvvG 357
gi 198424229 249 MQAAGksNLKRVTLELGGKSPNIIFEDa-DLDYAVEMAHFGLFFNmGQCCCAGSRVFVQesIYDEFVKKSVARAKKRtvG 327
gi 8131883   247 MQAAArtNLKRVTLELGGKSPNVVFSDa-DLDIAVEACHNGLFFNmGQCCCAASRTYVQedIYDVFTKKLVEKAKTRvvG 325
gi 156396842 249 QEASAksNLKRLTLELGGKSPNIVFADs-DMDYAVEMAHEALFFNqGQCCSAGSRTFVQdtIYDEFVKKSVKRAKARtvG 327
gi 196001343 248 AQAVGasNLKKISLELGGKSPNIIFDDv-DVDEALQWAHLGVFFNnGQCCTAGSRVFVQdaIYDEFVAKAVAKAKTVrvG 326
gi 2494070   252 IQGSGktNLKRVTLELGGKSPNIIFPDs-DLDYAVEVSHQAIMANmGQVCCAGSRTFVHedIYEEFVRRSVERAKKRtvG 330
gi 156551115 249 LEASAksNLKKVSLELGGKSPLVVFNDa-NLELALKYASDALFVNaGQTCIAPTRVFVQsgVYDKFTKRFVEIASKVkvG 327
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                        #  #                                              #             
1CW3_A       327 NPFdsktEQGPQVDETQFKKILGYINTGKqEGAKLLCGGGIaadRGYFIQPTVFGDVqdgMTIAKEEIFGPVMQILKFKt 406
gi 37958173  321 NPFdsktAFGPQVDKIQMNRILEMIESGKqEGARCVAGGNRmdkRGYFVEPTVFADVtdgMRIAREEIFGPVQQILKYKt 400
gi 114051966 321 NPFdd-vQQGPQIDEEMYKKVLNYIVAGKeGGARCVAGGDKvgdKGYYIKPTVFADVqddMKIAREEIFGPVQSILKFEt 399
gi 115899350 358 DPFdmskDQGPQINDEQFKKILGLIESGKsDGAKLATGGGRhgdKGYYIQPTVFTDVkddMKIAREEIFGPVQSIFKFSe 437
gi 198424229 328 SPFetgvEQGPQVDAEQFKKIQELIQSGKdEGAELLCGGERhgdKGYYIQPTVFGNVkdhMRIATEEIFGPVQQIMKFKt 407
gi 8131883   326 DPFdsrsMSGPQVDQEQFDKILALIDSGVqQGAKLECGGKRhgnVGYFVQPTVFSGVtedMRIGKEEIFGPVQQIIKFKt 405
gi 156396842 328 DPFds-vEQGPQIDQEQFDKIMDLIESGKkEGAKMQCGGARhgnKGFFIQPTVFSDVtddMRIAKEEIFGPVQQLIKFKs 406
gi 196001343 327 NPFdd-iDQGPQIDEEQMKKILELIDSGKaEGADMKCGGSRhgdRGYFIQPTVFTDVkddMRFAKEEIFGPVMQIFKFSt 405
gi 2494070   331 DPFdpknENGPQVDETQLKKILELIESGKtEGAKLECGGKRlgdKGYFVEPTVFTDVtssMRVAKEEIFGPVQLIFKFKd 410
gi 156551115 328 DAFepgvFQGPQIDTKGFNKVLSLIETGKkEGAKCEIGGKRrgnVGYFVEPTVFTNVsddMTIAKEEIFGPVQSIFKFEt 407
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1CW3_A       407 IEEVVGRANNSTYGLAAAVFTKDLDKANYLSQaLQAGTVWVNCYd--vFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKT 484
gi 37958173  401 LDEVIERCNDTNYGLGSAILTNDINEAMKFSRsIRAGSVWINIPymipVSVQTPFGGFKESGVGRELGEDGLRGYGEIKT 480
gi 114051966 400 FDEVVDRANDTNYGLAAGIITNDITVAMSFSKhVRAGSIWINTYe--hVTTQTPFGGFKDSGLGRELGEDGISQYLETKT 477
gi 115899350 438 MNEVINRANNTEYGLAAAVFTKDIDKALYVSNsLRAGTVWINCYn--vVSPMAPFGGYKMSGTGRELGEYGLEAYTEVKT 515
gi 198424229 408 MEEVIERANNTVYGLAAAVFTKDLNTAITVSNsVRAGTVWVNTFn--sFSSMTPFGGYKMSGQGRELGEYGLANYTEVKC 485
gi 8131883   406 MEEVVQRANKTTYGLAAAICTKDIDKVMTYSSqVKAGTVWVNSFn--vFGAQCPFGGFKMSGIGRELGESGLQQYSEVKT 483
gi 156396842 407 VDEVIERANNTTYGLAAGVFTKNIDTAIAVSSgLRAGTVWINCYe--cGAPQAPFGGYKMSGYGREWGEYGVLPYCEVKT 484
gi 196001343 406 IDEVIERANNTVYGLAAAVFTKDIKRAIHVSNhIRAGTVWINCYh--mTACQAPFGGYKMSGFGRELGEYGLELYSEVKT 483
gi 2494070   411 VDEVIERANDTSYGLAAAVFTKNIDTALKVANsLEAGTVWVNTYn--hFAFQAPFGGYKMSGQGREFGHYGLEAFLEVKT 488
gi 156551115 408 LDEVIKRANNTPYGLSAGVFTENLNTALEFSKaVQAGTVWVNQWg--aVHPQTPFGGYKTSGLGREMGIGSLDEYLETKT 485

                 ....
Feature 1            
1CW3_A       485 VTVK 488
gi 37958173  481 VVIM 484
gi 114051966 478 ITMH 481
gi 115899350 516 VTVK 519
gi 198424229 486 VIIK 489
gi 8131883   484 IIIK 487
gi 156396842 485 VTMK 488
gi 196001343 484 VTIA 487
gi 2494070   489 VYVR 492
gi 156551115 486 INIS 489

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap