Conserved Protein Domain Family
Patatin

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cd07198: Patatin 
Patatin-like phospholipase
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes PNPLA (1-9), TGL (3-5), ExoU-like, and SDP1-like subfamilies. There are some additional hypothetical proteins included in this family.
Statistics
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PSSM-Id: 132837
View PSSM: cd07198
Aligned: 19 rows
Threshold Bit Score: 93.1748
Threshold Setting Gi: 15800735
Created: 2-Jan-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              ## #                            #                                          
gi 116242718    6 LSFAACGFLGiYHLGAASALCRhgkklvkdvkaFAGASAGSLVASVLLTapekieecnqft------------------- 66
gi 152032658  928 LVLGGGGARGcAQVGVLKALAEcgip----vdmVGGTSIGAFVGALYSEernysqmrirakqw----------------- 986
gi 2501686   1373 LVLGGGGARGiSHLGVIQAIEEqgip----vdvIGGTSIGSFVGGLYAKdydlvpiygrvkkf----------------- 1431
gi 197624980    6 LALGSGGARGwAHIGVIRALQEegig----isgIAGASIGALVGAMYCAgelddledfvrd------------------- 62
gi 61250928   309 LVMAGGGARGlAHFGVYQELTEagvv----idrFGGTSSGAIASAAFALgmdagdaiaaar------------------- 365
gi 158517735  399 LCLSGGATFAyYHFGVVKALLEvdyl----pdiITGTSGGALVAALVATrtndelkellnpalahkitacresftvwfwr 474
gi 15237603   232 LLLSGGASLGaFHVGVVRTLVEhkll----priIAGSSVGSIICAVVASrswpelqsffenslhslqffdqlggvfsivk 307
gi 74626314   182 LYFNGGTAFGlYHFGVAKTLWKrnll----pqiLAGCASGALIASLLSVyrdeelnglfdtfpselwkicqqtsdysl-s 256
gi 732119      27 LVCEGGGQRGiFTAGVLDEFMRaqfn---pfdlYLGTSAGAQNLSAFICnqpgyarkvim-------------------- 83
gi 15800735     9 LVLSGGGAVGaYQAGVVKALAEcgtq----ismVSGTSIGAFNGAIIAAspdlsea------------------------ 60
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 116242718   67 ----------------ykfaeeirrqsfGAVTPGYDFMarlrsgmesilp------------------------------ 100
gi 152032658  987 ---------------aegmtslmkaaldLTYPITSMFSgagfnssifsvf------------------------------ 1021
gi 2501686   1432 ---------------agrissiwrmltdLTWPVTSYTTghefnrgiwktf------------------------------ 1466
gi 197624980   63 -----------------lrwrdlltyfdMVFPTSGLLDgnrvyellsehm------------------------------ 95
gi 61250928   366 -----------------efiagsdplgdYTIPISALTRggrvdrlvqgff------------------------------ 398
gi 158517735  475 wwktgarfdsvdwakqcawwchgsltfrEAYERTGRILnvscvp------------------------------------ 518
gi 15237603   308 rvmtqgalhdirqlqcmlrnltsnltfqEAYDMTGRILgitvcs------------------------------------ 351
gi 74626314   257 kvveygnmldismiasfvrqrlgtitfqEAFERTGRIVnivapp------------------------------------ 300
gi 732119      84 -----------------ryttkreffdpLRFVRGGNLIdldwlveatasqmpl--------------------------- 119
gi 15800735    61 ---------------------avrlealWEHLGNNQVLsvnrlvyfsllkklfqamnlcqipgragallttllrhistin 119
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 116242718  101 -------------------------------------------------psahelaqnrlHVSITNAKTrenhlvstfss 131
gi 152032658 1022 ------------------------------------------------kdqqiedlwipyFAITTDITAsamrvh----t 1049
gi 2501686   1467 ------------------------------------------------gdtriedfwiqyYCNSTNITDsvqeih----s 1494
gi 197624980   96 ------------------------------------------------qamriedaaipfCCVATDLIRgkevql----r 123
gi 61250928   399 ------------------------------------------------gntliehlprgfFSVSADMITgdqiih----r 426
gi 158517735  519 ------------------------------------------------------sdphspTILCNYLTSpdc-------- 536
gi 15237603   352 ------------------------------------------------------prkhepPRCLNYLTSphv-------- 369
gi 74626314   301 ------------------------------------------------------savsgsPQVLNYFTApnv-------- 318
gi 732119     120 ----------------------------------------------qmdtaarlfdsgksFYMCACRQDdyapnyfl-pt 152
gi 15800735   120 gfdnlmaqpllsdepltalmdhyldtdaladglplyvslypteggmqdiidciraelgvgTTKNAVFQHiqslp------ 193
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                 #                       
gi 116242718  132 redliKVLLASSFVPIYAglklve-------------------------ykgqkWVDGGLTNALPilp----------vg 176
gi 152032658 1050 dgslwRYVRASMSLSGYMpplcdp-------------------------kdghlLMDGGYINNLPadvar-------smg 1097
gi 2501686   1495 fgyawRYIRASMSLAGLLpplee---------------------------ngsmLLDGGYVDNLPvtemr-------arg 1540
gi 197624980  124 sgcmvDAVRASISVPGIFtpfcd---------------------------gdrfLGDGGIVNPVPvdvar-------dlg 169
gi 61250928   427 rgsvsGAVRASISIPGLIppvhn---------------------------geqlLVDGGLLNNLPanvmc-------adt 472
gi 158517735  537 --viwSAVLASAAVPGILnpvvlmmknrd--------------gslepysfghkWKDGSLRTDIPiks----------ln 590
gi 15237603   370 --viwSAVTASCAFPGLFeaqelmakdrsgeivpyhppfnldpevgtksssgrrWRDGSLEVDLPmmq----------lk 437
gi 74626314   319 --liwSAVCSSNSWAAIYrsspllaklpdgs----------tevctpknfiwpyAGLPNTGRSNPyar----------is 376
gi 732119     153 kqnwlDVIRASSAIPGFYrsgvs--------------------------leginYLDGGISDAIPvk------------e 194
gi 15800735   194 rgqqkEALLASAALPLLFrprev---------------------------qgtmFGDGGMGGWRNmqgntpvtplvdagc 246

                  ....*..
Feature 1                
gi 116242718  177 rTVTISP 183
gi 152032658 1098 aKVVIAI 1104
gi 2501686   1541 cQTIFAV 1547
gi 197624980  170 aELVIAV 176
gi 61250928   473 dGEVICV 479
gi 158517735  591 lHFNVNF 597
gi 15237603   438 eLFNVNH 444
gi 74626314   377 eIFNVNH 383
gi 732119     195 aARQGAK 201
gi 15800735   247 nMVIVTH 253

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