1OXW


Conserved Protein Domain Family
Pat17_PNPLA8_PNPLA9_like

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cd07199: Pat17_PNPLA8_PNPLA9_like 
Click on image for an interactive view with Cn3D
Patatin-like phospholipase; includes PNPLA8, PNPLA9, and Pat17
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes subfamily of PNPLA8 (iPLA2-gamma) and PNPLA9 (iPLA2-beta) like phospholipases from human as well as the Pat17 isozyme from Solanum cardiophyllum.
Statistics
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PSSM-Id: 132838
View PSSM: cd07199
Aligned: 92 rows
Threshold Bit Score: 111.269
Threshold Setting Gi: 125545017
Created: 7-Nov-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitenucleophile
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               ## #                                      #                               
1OXW_A         18 VLSIDGGGIRG--IIPATILEFLEGqlqexdnnadarladyFDVIGGTSTGGLLTAXIStpnen--nrpfaAAKEIVPFY 93
gi 73666815     5 VLSVDGGGVRG--IVAATILQEIEKrinkp-------lckvFDLVSGSSVGSLICGALCvknad--gtprySACDLLELI 73
gi 3861146     14 LALLEGGGVKG--EIHLEKLKVIEEitgkp-------tckvFDFTGGTSVGGLILILLNlpdsdnpgkplfSAEQAQTLF 84
gi 67459804    41 ILSLSGGGVKG--IAELVVLAEIEErtgks-------iselFPIISGTSVGGLIAGLLTipkeqgsniakySAKDALKIF 111
gi 210098381  619 VLALDGGGSKG--IFQAMVLDAVYKkitpfap--gipfrelFDLIIGTSIGSAIGAAIGrm--------nfSPIDARRLI 686
gi 4678298    100 ILSIDGGGMRG--ILPGKALAYLEHalksksgdpnariadyFDVAAGSGIGGIYTAMLFgsrdg--nrpifKADDTWQFL 175
gi 157354187   60 ILSIDGGGATDg-ILAARSLAHLEAslrhksgnpnarisdyFDVVAGSGAGGILAALLFtrgkd--ggplfSADEALRFL 136
gi 115451971   77 VLCVDGGGGGLraLLSGRALAHLEAalrrasgdpdarvadyFDLVAGTGAGGVFAAMLFsthsr--gaplfHADDTWRLV 154
gi 168003996   24 VLSLDGGGMHG--IIPARLLAHLEEllkqksgdqdariadyFDVVTGTSVGGLIATMLFtgden--srplfRAEEAWRLM 99
gi 168041578   32 ILSLDGGGMRG--LIAARILSHLENilqekvge-kvklcdyFDLLAGTSTGAVLATMLVtpdan--gnptfTAEGCCEFY 106
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1OXW_A         94 FEHGPQIFNpsgqilgpkydgkylxq------------------------------------------------------ 119
gi 73666815    74 LMYAGKIFCnstvrnalslvfgpkysdkn--------------------------------------------------- 102
gi 3861146     85 ERMVHNIFPegltfrklwscnglfsykfsp-------------------------------------------------- 114
gi 67459804   112 TDAAPKIFEhhwydgikqifthkhsqgp---------------------------------------------------- 139
gi 210098381  687 EDSVSTIFVksgwrrfsgttlavlsgtiysnknlqklledgfgdttlsgapvkvgsraslsstdslsqsqtsetlrprsl 766
gi 4678298    176 TRNAKGLYGgagilkrvlrtgsgccsgta--------------------------------------------------- 204
gi 157354187  137 VENRRRIFRappagvlrrmfrpakae------------------------------------------------------ 162
gi 115451971  155 ADHAPRLFRkavggstslfcrpkkrplaapt------------------------------------------------- 185
gi 168003996  100 AERGREVFKkvsrssvwsklrwkssktswfsaks---------------------------------------------- 133
gi 168041578  107 KKNGRLIFQhrwydpfhgsvrqlyrpkysgrr------------------------------------------------ 138
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1OXW_A        120 -------------------------------------------------------vlqeklgetrvhqaltEVVISSFDI 144
gi 73666815   103 ----------------------------------------------------lnavlqeifgdvtikdliaDFIVPSYDL 130
gi 3861146    115 --------------------------------------------------dplvkllkkyckdytlkdligEVIVTGYDL 144
gi 67459804   140 ----------------------------------------------------lkeileqhlaglrlddttsRLIIPVTDL 167
gi 210098381  767 rsvskrlsmsvdmlqelkeecehtlfqhtrrrpslvvkndhkmfqhvgrrssivieeeeeeftpptdlvlpVVACCVYDM 846
gi 4678298    205 ---------------------------------------------------klkkvmkesfseltlkdtlkPVLIPCYDL 233
gi 157354187  163 ------------------------------------------------------kvfqrafgeatlkdtlkSVLITCYDL 188
gi 115451971  186 -------------------------------------------------aaldaamktafgeeltlrdtikPVLISCYDL 216
gi 168003996  134 -----------------------------------------------leqvfkeylmrkdgsaltlrdtikPVLIPCYDL 166
gi 168041578  139 ------------------------------------------------fedllkkytfidgkfltlldtlkPLVVTSFDI 170
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                       #                 
1OXW_A        145 k--TNKPVIFTKSnlanspelDAKXYDISYSTAAAPTYFPPhyfvtnts---ngdeyefNLVDGAVATvADPALLSIsva 219
gi 73666815   131 c--SNQTIMFRSWidk---ysDIKVCDVTRAAVAAPTYFTPkkmiv--------dnkkqLLVDSAIVC-NNPVIAAYsaa 196
gi 3861146    145 nnqQNPLITFSTIearksqdnNYYLSDIIQGITAAPGYFPShhfsni------tntkvhTIIDGGIYA-NDPTLQTWqll 217
gi 67459804   168 askDKEVKIFDSHdsy---spHIRVQDVLLATTAAPTYFKPvtnqehikgyenqedalyAYADGGLGA-NRPAYEALkil 243
gi 210098381  847 d--RVKTVTLSGHd------cSLKAREAVLASTAAPTYFPSvqlly--------kgekrQFCDGAIGA-NCPAGEGKkla 909
gi 4678298    234 k--SSGPFLFSRAdaletdgyDFRLSEVCRATWAEPGVFEPvemksv------dgqtkcVAVGGGLAM-SNPTAAAIthv 304
gi 157354187  189 s--TRAPFLFSRAdaleidgyDFKMKDVCVATSSDPTVAGGvelrsv------drgtriMAVDGRIAM-NNPTAAAIthv 259
gi 115451971  217 k--SSAPLVFSRAdalesesyDFRLCEVGRAAWSEAGRFEPaevasv------dgatscAAVDGGPIM-GSPAAAAIthv 287
gi 168003996  167 a--TAAPFLFSRAdalesqtwDFNLWEICRATTATPPFFPPacvtsv------dgntscTAIDGRVVM-HNPTAAAIthv 237
gi 168041578  171 s--QATPFFFVRQaaqkdqsrNFRLWEVCRATAAAPTYFPPasvrsv------dgrvqgTLIDGGAVQ-NNPALVATtha 241
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1OXW_A        220 trlaqkdpafasirslnyKKXLLLSLGTGTTSEfdktytakeaat--wtavhwxlviqkXTDAASSYXTDYYLStafqal 297
gi 73666815   197 qv------------lypnEKICCLSVGCGTVSQsfsdlqnsl--------lywsrkilcVIIDAGLEAIDYEMArlv--- 253
gi 3861146    218 ke-----------nkciiDNALYLSLKEENNADyktvccgggil-----ellknnmpnkILAATQQADEITAQRif---- 277
gi 67459804   244 knghs-----reenakilDDTMVLSLNFDNDHKasssipk-------------igfdgvIGWLVKGKLVSRLMQsse--- 302
gi 210098381  910 ke-----------vwpdrRIDTMLSVGCGGDATsgaskpns----------iatigaalLNFVVRTEQHWNELEk----- 963
gi 4678298    305 lhnkq-----efpfvrgvEDLLVLSLGMGQLLDvsyeydriikw----kakhwarpaalISNDGAADTVDQAVAmafg-h 374
gi 157354187  260 lnnkq-----efpfcnsvGDLLVVSLGNGESDSgagnlss------------taselvrIAGEGASDMVDQAVSmafg-e 321
gi 115451971  288 lhnkh-----efpfvrgvEDLLVLSIGGCSAGGtgaaadadlrrlrrwgpkdwarpiarIAADGAADLVDHAVArafg-q 361
gi 168003996  238 lhnkr-----efpfvrgvEDLLVLSLGTGQIDQtymydtvrgwg-----tmqwvkslakIVLDGVADTVDHTISmaf--g 305
gi 168041578  242 isnne-----efpyvnglEDVLILSIGAGQMDKkhdlqkarkwg-----mtkwvrpimdIMMDGTADTVDYQLAaayagn 311
                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....
Feature 1                                                     
1OXW_A        298 dsKNNYLRVQENAltgttte---xddaseANXELLVQVGENLLK 338
gi 73666815   254 kgEDSYCRISGDIvysacd----fgdasqENIKNLKKDAQKILQ 293
gi 3861146    278 gnKLIEIPTYIPEqhaem------snssiENLQALKEFAKKSIY 315
gi 67459804   303 dsSTAEVRVGLPGedefve----vklpitKETSSLDNSSLKNIE 342
gi 210098381  964 -dNDCFVRLDAPArvgige-----lplncKDVDKIKRYALSVQC 1001
gi 4678298    375 crSSNYVRIQANGsnlgpwspnmdtdpsgSNVNMLMGVAEEMLK 418
gi 157354187  322 srATNYVRIQGNAmlgkr------hsnglKDSKQLVGMTEEMLE 359
gi 115451971  362 crSSNYLRIQAKResmppcgpdgeydptpANVHALLAAADEMLK 405
gi 168003996  306 ehRKHYLRIQVTYpgpglpn---ktetdvNDVNQLLKLSEDLLD 346
gi 168041578  312 ncSENYLRIQLSGlpnktsv---mdcatqKNIHDLITISDDLIK 352

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