Conserved Protein Domain Family
Pat_hypo_Ecoli_yjju_like

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cd07208: Pat_hypo_Ecoli_yjju_like 
Hypothetical patatin similar to yjju protein of Escherichia coli
Patatin-like phospholipase similar to yjju protein of Escherichia coli. This family predominantly consists of bacterial patatin glycoproteins, and some representatives from eukaryotes and archaea. The patatin protein accounts for up to 40% of the total soluble protein in potato tubers. Patatin is a storage protein, but it also has the enzymatic activity of a lipid acyl hydrolase, catalyzing the cleavage of fatty acids from membrane lipids. Members of this family have also been found in vertebrates.
Statistics
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PSSM-Id: 132847
View PSSM: cd07208
Aligned: 49 rows
Threshold Bit Score: 172.794
Threshold Setting Gi: 149926816
Created: 14-Nov-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             ## #                              #                                        
gi 732119     27 LVCEGGGQRGI-FTAGVLDEFMRAQFn----pFDLYLGTSAGAQNLSAFICNQPGyarkvi------------------- 82
gi 159473607  54 LVVEGGGMRGC-ISGAMLMALHDLGIsq---vFDVVYGASAGAINATYFLTGQRNglriyh------------------- 110
gi 149926816  12 LALAGGGPLGAiYEIGALVALSEAIDgldlndMSVYVGVSAGGIVATGLAHDLTPhqiyklfiegepee----------- 80
gi 120553749  15 LALGGGGPLGGiYEIGALRALDEALDgldfnnIDVYVGVNAGSFVAANLANQMTTaqlcrifvrneaevh--------pf 86
gi 54022955   22 LVIEGGGSRGV-YSSGMVQGLEELGLsa---vFDAVYGTSAGAINGAWLLCGRAVpgmrsw------------------- 78
gi 27365611    4 LVVEGGAMRGV-FASGVLDAFLQKNYq----pFDLAIGVSAGASNLVGYLANAQGrsfnvi------------------- 59
gi 196156692   5 LVVEGGAMRGI-FAAGVLDKFMEHDYy----aFDFTLGVSAGATNLSTYVSKMPGlsktii------------------- 60
gi 163848819   8 LVLAGGGVAGAaYEIGALCAIDQVLEhlsvneFDVYVGTSAGALISACLANNMSPrtlisvlessllgidqlephhlyql 87
gi 186471411 104 LAISGGGDNGA-FAAGLLNAWTETGTrp---eFKLVTGISTGALIAPFAFLGEKYdat---------------------- 157
gi 168704380  99 LVVTGGGSYGA-YPAGVLVGWTATGTrp---eFDVVTGISTGALLGAFAFLGPGE------------------------- 149
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 732119     83 --------mryttKREFFDPLRFVRGGNLIDLDWLVeatas------------------------------qmplQMDTA 124
gi 159473607 111 --------edltqGTAFLDFRNLFSSSGAPVMNVDFlidqv------------------------------mntsKPLDW 152
gi 149926816  81 lafdpsillrpawKEYWMRLRKVPSLLGTAVSDYFLrddatwvscierlgaaiptglfngdelhewmadliakggGTNDF 160
gi 120553749  87 hpevfyrpalreiGRRLLAVPGLLSTAVSRFVNNPYdqslleamtilaqaapagl--fdneglheylkraftmlgRTNDF 164
gi 54022955   79 --------snpdiMRAVIDPARLLRGGAAFDVRYLVhrvy--------------------------------dgvEPMDF 118
gi 27365611   60 --------tklatSQRFYNPKRFIKGGDLVDVKWLVdes---------------------------------mrrYPIEA 98
gi 196156692  61 --------tqyatKREFFSPLRFIKGGHMTDVHWLWhh-----------------------------------skQSMNI 97
gi 163848819  88 nitdllyraahlpGALVQAIQRWVREGGEVSLLDLIetvavglptglyd-------------srsleqylraaleQPGRS 154
gi 186471411 158 ----------lkqVYTTISPNDVLEKRSLLGGVLSDamadnrpll----------------------altrkfvtEDLLK 205
gi 168704380 150 -------------DAELQRCYTTLRDRDIYKRNRIIpsllseslad--------------------sgplaqvieRTAND 196
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 732119    125 ARLFDSgk------sFYMCACRQDDYAPNYFlptk----------qnwlDVIRASSAIPGFYRSg-------VSLEGINY 181
gi 159473607 153 DGVLTSpa------pLKVVASCLDSLQPVIIsdfad--------raelaEALKATAAVPQIAGPp-------RQLRGRQL 211
gi 149926816 161 RSLRNR---------LILVATDLDSGDSIPFgmpgfd-------dvpisKAVQASASLPGLFPP--------TRIANRHY 216
gi 120553749 165 RQLKRS---------LYVVAADVESTEAVCFgapgyd-------hvpisRAVQASTSSPGLYVP--------VEIDGRFY 220
gi 54022955  119 DAILNHat------tFHPIATDARTGNAVDLrphiyd-------krslmRALRASANLPILAGGp-------ILLDGVPY 178
gi 27365611   99 ERLFTNt-------rLLATTTDIATGLPHYQqltk----------qnvnQLLEASCALPIAYRQp-------PCFAGSCF 154
gi 196156692  98 PEPGEKsc-----mpLFVGITNADSGECEYIqatk----------dnvdDLMVASCALPTAYRDe-------ITINGTRY 155
gi 163848819 155 NRFADLdh------eLMIVATDLDTGERAIFglppld-------nvpisLAVCASAAIPIFYRP--------VRIGDHDY 213
gi 186471411 206 EIAAEYakg----rmLLVGTTDLDSRRGVIWdmgkiatyggpaaldlfvKVLIASTSIPGAFPPmmidvevgGKRYQEMH 281
gi 168704380 197 ERIARFaaehakgrrYYVGTTDLDARRAVVWdmgaiaarntpesrelfrKVLLASAAIPGFFPPvripmtvdGRRYVERH 276
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #                                                                              
gi 732119    182 LDGGISDAIPVk-----------eAARQGAKTLVVIRTVPSQMYYtpqwfkrmerwlgdsSLQPLVNLVQHhe------- 243
gi 159473607 212 VDAAVFEPVPVp-----------sAIRDGCTHVLVLCTRPAPTQRspw---------aqrMRSTIEAMAKStvlnapymr 271
gi 149926816 217 VDGALKKTLHAs-----------tALKEGVELLICLNPLVPYDDRlnpknhr--rrakgkAFQPGSRLYEGglplvlsqt 283
gi 120553749 221 VDGTLRKGLHAs-----------iAFEDGADLVLAVNPQVPIDASaavragt--mkpgelTRSGMPNLLSQmfrtm---- 283
gi 54022955  179 FDGGIAEPIPIh-----------aAVRGGGTHFLVLRTRRADESRppasrvh-rlvgggyLRRRAPGAYRAwla------ 240
gi 27365611  155 TDGGVSDSIPVk-----------eAYRQGARDITVVLSHPLSYEMpesrht----wltntLLSRQPAIAKAlrqr----- 214
gi 196156692 156 LDGGVADAIPAi-----------qAYKMGARDITVILSQPVGFTKpevksp----wlmqkMYKNQPALLEKmlqr----- 215
gi 163848819 214 IDGGLRGTASLd-----------vAIERGAELIVCINPMVPFDNRrhppga---aisdqgIQRIGNQVFRTfiha----- 274
gi 186471411 282 VDGGVVAQVFAypa--------tlRVAEEASALGVIRERKLYIIRnarldsd-wmqvqraTMRIASRAVESmiqsq---- 348
gi 168704380 277 IDGGTTSSMFFappyappghepppGWLHGSNLYILVAGKLYPDPSpvk----------prTFAIASNAVSTviydq---- 342
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
Feature 1                                                                                   
gi 732119    244 ------------------tsYRDIQQFIEKPPGKLRIFEIYPPkpl------hsiALGSRIPALREDYKLGRLCG 294
gi 159473607 272 eawrtarspdivswdqqlvaTLHGCPHEVQRSMGAYVLPLYPEhta------gchPLCLDPNTLLAACDVGRQAL 340
gi 149926816 284 ------------frsiihsrMDIGMERYGHLYPNADIVLFEPQhgdgeffftnpfSYASRRRLCEHAYQRTREEL 346
gi 120553749 284 ----------------vysrMQSGIAQYTRDYPDKDILLFEPTrddaklffsnvfSFQSRRMVCEHAYQMTRRDL 342
gi 54022955  241 ------------------rpDQERAEDRLLADLGEKVWQIHPPpgsp-----tvdGTARDTGLLSRALELGRRAV 292
gi 27365611  215 -----------------aqnYNAALAFIRFPPADATIRVIAPEegf------kvkRLTMNQSKLHQGYQMGITQG 266
gi 196156692 216 -----------------asiYNDTLAFLNSPPSDCTIRVIAPDeaf------cvkRLTMDKKKLLKGYGQGRRMA 267
gi 163848819 275 -----------------glhYHIKQVRRRHPEVDIILIEPSRQdrt------mfaENTMRFHTRMTIARHGFESV 326
gi 186471411 349 ---------------gvgdlYRIYATANRDGVDFNLAFIPSSFqa--------phEEEFDTEYMRALYYTAYNMA 400
gi 168704380 343 ----------------trsdLHKLFLLTAMTGMTYNVSVIPPEins------pleSTKFDPKEMSRLFCAGAQWA 395

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