Conserved Protein Domain Family
Pat_hypo_Ecoli_Z1214_like

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cd07209: Pat_hypo_Ecoli_Z1214_like 
Hypothetical patatin similar to Z1214 protein of Escherichia coli
Patatin-like phospholipase similar to Z1214 protein of Escherichia coli. This family predominantly consists of bacterial patatin glycoproteins and some representatives from eukaryotes and archaea. The patatin protein accounts for up to 40% of the total soluble protein in potato tubers. Patatin is a storage protein, but it also has the enzymatic activity of a lipid acyl hydrolase, catalyzing the cleavage of fatty acids from membrane lipids. Members of this family have also been found in vertebrates.
Statistics
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PSSM-Id: 132848
View PSSM: cd07209
Aligned: 51 rows
Threshold Bit Score: 145.127
Threshold Setting Gi: 118469544
Created: 2-Jan-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             ## #                                #                                      
gi 15800735    9 LVLSGGGAVGAYQAGVVKALAECGt--------qISMVSGTSIGAFNGAIIAASpdls--eaaVRLEALWEHLGNNQVLs 78
gi 111018895   5 FVLSGGASLGALEVGMLQTLIGRGi--------rPDLVVGTSVGALNGAWLAGGssd---adlRELADLWRGLTRSAVFp 73
gi 199597156   5 VVFGGGAAHGAYQAGVWSVLGPRLa---------PAFVIGSSIGAINAAATAHMa-------pQQLAHWWQSLTSLKTRg 68
gi 17544940    9 FVFAGGGSLGAIEAGMLRELVARGv--------mPDMVVGASAGAINAAFFACHphm---dgaARLEALWRGVRRAEVMp 77
gi 126347709  39 VVVGAGGVLGAAHVGVGHALEQRGf--------rPDMIIGTSVGALNGAIAAAHpdr----aaPWLEHVWTELRRRDVYq 106
gi 6689533    12 LVLCGGGARGAMQVGFYQAIIELGl--------sFDLVVGSSVGALNGTLIAAGms------pAELSELWQGLRKPDVVs 77
gi 118469544   3 FVLGGGGVLGAAEAGMALALLESGv--------rPDLVCGTSVGAINGAALAADptp---egaRALVKFWDALGGDGVFg 71
gi 159901372   4 IVFSGGANRGSLQAGAALALLEAGi--------iPDLVVGSSVGSINGAVLACHpnl---agiQRIAQRWKSVKREDIFp 72
gi 134102779   5 WVLPGGSTFGAIQAGLVTSLFEAGe--------aPDAVIGTSVGSLNAAWLAGDpta---rgaEKLREVWLSMRRSDVFa 73
gi 118355534  28 LVIAGGADLGSYEAGVIQGLVQAYnelgqvsevyWDVISGVSVGAITAVGLGQFpkeqtvemaNWVVNLWENISKSDVYs 107
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 15800735   79 vnrlvyfsllkklfqamnlcqipgragallttllrhistingfdnlmaqpllsdepltalmdhyldtdalADGLPLYVSL 158
gi 111018895  74 tglltgftgfv------------------------------------------------glrnhlvsnraIRRLLEQNLR 105
gi 199597156  69 pifar------------------------------------------------------------rnfvpLLEQMLRLPK 88
gi 17544940   78 wswrvmlgm----------------------------------------------------lgrrsthlvDAGGLQRLLN 105
gi 126347709 107 lgfpssr-------------------------------------------------------asiftdrgLRRLIARAGL 131
gi 6689533    78 wnwrgllge----------------------------------------------------tggfysfapLRRLLHEALP 105
gi 118469544  72 gslirraaevl------------------------------------------------rrptslhdnseLRRLLRERLP 103
gi 159901372  73 ggslrsawrvv------------------------------------------------rgrgslhhnhgLRRFILSLLP 104
gi 134102779  74 iqpmrilagklgltnh--------------------------------------lmsnrglarwlyqtlpYRRLEDARIP 115
gi 118355534 108 nyl----------------------------------------------------------------ggvVQGFLFEESL 123
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                               #        
gi 15800735  159 YPTeggmqdiid------ciraelgvgTTKNAVFQHIQslpr-gqqkEALLASAALPLLFRPreVQGTMFGDGGMGGWRN 231
gi 111018895 106 FERmedapip-----------lhviaaDVLTGKDVRLStgp----avDAVTASAALPGILPPvvIDGRALMDGGVVDNTP 170
gi 199597156  89 RDQravla--------------vttrlPDLRAHVTRIDelsv-adarEWLLASSAMPGIFTPidRHGTSFVDGGVVNDLP 153
gi 17544940  106 RHFaqarleaap-------lplhvvatDMQTGAEVVLStgs----ivQAVLASAAIPGVFPPvrVDGRLLIDGGVANNTP 174
gi 126347709 132 PSRieelavp-----------ftavamDLVTGAPALLDhgd----leSALLASAAIPGILPPvdREGRTLVDGGMTAYVP 196
gi 6689533   106 VKRfeelkhp-----------lvitttNLQSGQPCYHErhg---vliEPMIASMSLPGVFPPvwIAGDQHVDGGVTDNVP 171
gi 118469544 104 VHTfeelavp-----------fecvaaSIERAREHWFDsgd----lvEPVLASCALPGVLPPvrIGDEHFFDGGLVNSIP 168
gi 159901372 105 PNIrrfsdlai---------pffvtatNYRTGDMHLFGddpr-erlvDALMASAAVPPYLPAyhYRGETYLDGGFVSNLP 174
gi 134102779 116 LTVta---------------------tDIDSGEAVYFDrgp----alPALVASCSIPGMFPPikVGDRWLVDGGPAAFMP 170
gi 118355534 124 FKTdplraflkknvtrkqtrklvigvtNANTGEYERYNeslsdadliEAVMCSSAVPAFFPYqiFKGNTYFDGGVVRAAD 203
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 15800735  232 MQGNTpvtp------lvdaGCNMVIVTHLSDGSlwdr------qafPDTTILEIRPRKrlkyagdggnsggllsftsaht 299
gi 111018895 171 ISHAIelgattvwvlptgyACTLSKVPGSALGMgll-------gvtLAINQRLALDIEryesva--------------di 229
gi 199597156 154 VDIARd------------iGADFVVAISGAGIGrigs-----dhgdVYLKPSRPLPRLfnfrp----------------- 199
gi 17544940  175 ISTAIr------------lGATRVVVLPAGFACadrreprsaiehaLNALSLLVARQLaqdlehwag---------tipi 233
gi 126347709 197 VLAAMr------------aGAASVVVVPTGPESsp----------lPATTPPRRAGAIaarvglv-------------lm 241
gi 6689533   172 FDKALd------------rGAHEILMISCTCCPrd---------stALASPLKILLRS---------------------- 208
gi 118469544 169 LDRAVs------------rGADTIWVLHVGRLEeelrvp--rflweVGFAAFEIARRHr--------------------- 213
gi 159901372 175 IGVALq------------qGASEIWALEIGLDAaa---------tvPPFGVRGSLTRTfdalmrr-------------qm 220
gi 134102779 171 ISRAVe------------qGAERVYVLPCGGSEp-----------fEFDRRRRGVGTVatwpppqsppks---itgvnga 224
gi 118355534 204 VFGAIdrckelv-ddvsqiTVDVILVTSKTFEQd-----------vPDDLKPIKVGTLsqkvk----------------d 255
                        330
                 ....*....|....*.
Feature 1                        
gi 15800735  300 dawrQQGYEDTMLAME 315
gi 111018895 230 rvvpPLCPLDTSPADF 245
gi 199597156 200 aaitEMMALGKKDAEM 215
gi 17544940  234 svvpPLCPLEVSPYDY 249
gi 126347709 242 hrqiERDLREVSRRLP 257
gi 6689533   209 ----FMIALDTKYLCS 220
gi 118469544 214 ----FHGDLERVRSDV 225
gi 159901372 221 aherELVRLAAKAGVK 236
gi 134102779 225 amgaAMVAAARLDMQV 240
gi 118355534 256 hhnaRRSLLEAYEAFP 271

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