Conserved Protein Domain Family
Pat_PNPLA8

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cd07211: Pat_PNPLA8 
Patatin-like phospholipase domain containing protein 8
PNPLA8 is a Ca-independent myocardial phospholipase which maintains mitochondrial integrity. PNPLA8 is also known as iPLA2-gamma. In humans, it is predominantly expressed in heart tissue. iPLA2-gamma can catalyze both phospholipase A1 and A2 reactions (PLA1 and PLA2 respectively). This family includes PNPLA8 (iPLA2-gamma) from Homo sapiens and iPLA2-2 from Mus musculus.
Statistics
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PSSM-Id: 132850
View PSSM: cd07211
Aligned: 20 rows
Threshold Bit Score: 373.129
Threshold Setting Gi: 211965692
Created: 10-Feb-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                        ## #                             #                               
gi 74734299   435 DPVKGRGIRILSIDGGGTRGVVALQTLRKLVELTQKPVhQLFDYICGVSTGAILAFMLGLfh-mPLDECEELYRKLGSDV 513
gi 50508404   593 RPVAKKGLRILSMDGGGMKGLATVQILKQIEQGTGKRIhEMFDLICGTSTGGMLAMALGVkq-mTLDQCEEIYTKLGECL 671
gi 211965692 2023 RAGRRRGLRILCFDGGGTRGVLSLALLKQIMACVGQEVhETFDIICGTSTGGVIAALLGLek-aTVSEAERLYDLLIREI 2101
gi 156087559  873 NRFKNRGLRVLSIDGGGSKGVIVLEILLDLEKRLGRPLyEVFDVIVGNSCGGIIGAFLALek-sRVTDVQRYFDAMLGDV 951
gi 85001504   782 KTFENRGVRILSIDGGGSKGVVALEILDYLNKQLEKPLhECFDIICGTSTGGLLASLVALek-mEISEIQKLYDSMIKSI 860
gi 168024097  500 RPISKRGLRILSMDGGGMRGMATVQMLRNIEKGTGRRIhEIFDLICGTSTGGMLAVALGIkr-mDLDQCENIYKSLGKLV 578
gi 17544092   200 PSPKGKGVHVLSIDGGGTRGMMGLEVLEKIEKLSGKKIcELFDMICGVSTGSIIAALLTAkg-ySVKECREVYMDVSKRL 278
gi 212511637  179 NPLPSQGIRILAIDGGGIRGLLVMEMLAKFEELTGKKInELFDYICGVSTGSVIACTVGAsg-kSIDEISALYRELGNKI 257
gi 159489498  506 PPIRGRGLRVLALDGGGMRGLALVQILRHIEKRTGRPLhGLFDLVVGTSTGAIVAVGLGVfh-fSLDQCEAIYTGLGHKV 584
gi 157132785  116 PPLPGPGIRILSVDGGGIRGLIVAELLRRIEKMTGKKIfELFDMVCGVSTGAILLCALTSeknlTLDESIILYKKMSHKM 195
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 74734299   514 FSQnv------------------------------------------ivGTVKMSWSHAFYDSQTWENILKdrmgsalmi 551
gi 50508404   672 LALtfhiflivlpsellvspgklvfaepapkdeaatwkekidqlfksssQSFRVVVHGSKHSADQFERLLKemcadedgd 751
gi 211965692 2102 FVRdsa-----------------------------------------avTGARLVLRQALYNEKGWEGILErawgrrrmi 2140
gi 156087559  952 FKKegy-----------------------------------------gsKGKRLFTHLAYYNEQTLYDALTaafgnleli 990
gi 85001504   861 FVRdyy-----------------------------------------piTGTRLLFRHAIYDDTTLKDILKtsleeieli 899
gi 168024097  579 FAEpipkdneaatwr-----------------------ekidqvyksssQNFRVVVHGSKHNAEQFEHLLKemcadeegd 635
gi 17544092   279 FSQgk------------------------------------------fqGSMGLILKHSYYNTNLWISILKqmigeditm 316
gi 212511637  258 FSQnv------------------------------------------ffGARSLIWNHGYYDTALWEKILKehvgetpli 295
gi 159489498  585 FNQggwrdslf-------------------------------rvvrgtsTSLRVAVYGFKHDASTFEELLRqmc------ 627
gi 157132785  196 FHRpspl--------------------------------------dkitGASRMVLSHAYYDIELWESLLKqylgyrrii 237
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 74734299   552 -------------------etarnptcPKVAAVSTIVNRg---iTPKAFVFRNYGHFPGIn------------------- 590
gi 50508404   752 lliesavkgipkyppgtvevssvmtesPSIGSAGTPVSGap--vGIKPINTVGTAVSGAPvgi----------------- 812
gi 211965692 2141 -------------------dfaadpacPKVFCVSTVASPn----PTQVMVWRNYNFPVRLvpkaaaqprgretppngdsg 2197
gi 156087559  991 -------------------dysvdpdaPKFCCLSVQLDIy----PFKPVLWRSYNYPPNAeskk---------------- 1031
gi 85001504   900 -------------------dysvdstcPKFFCVSTQMDVt----PLRPIIWRNYNYHKDIyslgskdlt----------- 945
gi 168024097  636 l-----------------liesavkgvPKVFVVSALVSVt----PAQPFVFRNYQYPPGTpetapstndgpaasvsg--- 691
gi 17544092   317 ------------------intsrklhtPRLAIVSSIVNLp----TIQPYIFRNYDHPAGRd------------------- 355
gi 212511637  296 -------------------ktsrnhpyPKIGVISTVTNHd----QIVPYIFRNYELPYRVk------------------- 333
gi 159489498  628 -------------------------evKKLGCVGNQMIDaaalgGPKVAAVATLVSVCPV-------------------- 662
gi 157132785  238 -------------------dtsklpnvPKFCCVSTTICDe----HIEAHVFRNYTFPLNAh------------------- 275
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 74734299       --------------------------------------------------------------------------------
gi 50508404       --------------------------------------------------------------------------------
gi 211965692 2198 daseptakpeagresqrearrlgfvdwwrklgtkesgapqpeaagreardqdnsrlcrgsaqyspsqsvfsrfayffrpp 2277
gi 156087559      --------------------------------------------------------------------------------
gi 85001504       --------------------------------------------------------------------------------
gi 168024097      --------------------------------------------------------------------------------
gi 17544092       --------------------------------------------------------------------------------
gi 212511637      --------------------------------------------------------------------------------
gi 159489498      --------------------------------------------------------------------------------
gi 157132785      --------------------------------------------------------------------------------
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                     #   
gi 74734299   591 ----------------------------------------SHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGL 630
gi 50508404   813 --------------------------------------krGAFMGSCKHRIWEAIRASSAAPYYLDDFSDDVNRWQDGAI 854
gi 211965692 2278 htasgspsrqgrpdsfspyscsspcalpspssfllvpsrgSRHAGSSRILVKDALRATTAAPGFFSGIFWEGQAFSDGAL 2357
gi 156087559 1032 -------------------------------------nspRIIDGTFAVKTPDALRATSAAPTYFPMMEINGALYADGAL 1074
gi 85001504   946 --------------------------------iedlnkliEIIGGSCTIRLRDAIKATTSALGYFPLFERNGHLYGDGAL 993
gi 168024097  692 ------------------------tpatatplttqvgprrSAFIGSCKHRIWEAIRASSAAPYYLDDFSQESNRWQDGAI 747
gi 17544092   356 ----------------------------------------SHYRGGADHCLWTAIQASAAAPLYFSEVKLDNLLLQDGGV 395
gi 212511637  334 ----------------------------------------SKYLGSYKHQLWEATRASAAAPTYFEEFSLGDFLHQDGGV 373
gi 159489498  663 ----------------------------------------TPFLFTTYELPPEAVRASSAAPYYLDDFLCGEDRFQDGAA 702
gi 157132785  276 ----------------------------------------SVYSGSHTARMWEVVRASSAAPAYFGDFQLDGQLHQDGGI 315
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 74734299   631 LLNNPSALAMHECK-CLWPDVPLECIVSLGTGRYESdvr----------------------------------------- 668
gi 50508404   855 VANNPTIFAIREAQ-LLWPDTRIDCLVSIGCGSVPTksr----------------------------------------- 892
gi 211965692 2358 LANNPTAVALAEARgLYGEDVPIDLVVSIGTGKFPSsfsspsrgdslhhreeaat------------------paesdkg 2419
gi 156087559 1075 YANNPSAIAVIESK-LLYPDVPIDLLVSISNGDWNDmfsldaataaaagidiapsdteskdnnttervppldkiyndivl 1153
gi 85001504   994 YCNNPSVVALIESK-LLYPDTPVELLVSVGNGLSQFnpngsndpgncqsd---------------------------dvt 1045
gi 168024097  748 VANNPTIIALREAQ-LIWPDTQVDCLVSVGCGNVPTkar----------------------------------------- 785
gi 17544092   396 YANNPTAIAYHEAK-LLWPDENVNCVVSVGNGRTVTsvep---------------------------------------- 434
gi 212511637  374 LVNNPTALAVHEAK-QLWPNNEIQCVVSFGTGRLDPlsiesvk------------------------------------- 415
gi 159489498  703 TANNPGILALQQAR-LLWPNTPVEALVSVGCGAAPSvrr----------------------------------------- 740
gi 157132785  316 LYNNPTAVAIHEAK-CLWPNEPIQCVVSFGTGRTRTrdwkdgqkii---------------------------------- 360
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 74734299   669 --------ntvTYTSLKTKLs----------nVINSATDTEEVHIMLDGl---LPPDTYFRFNPVMcen-ipLDESRNEK 726
gi 50508404   893 ---------rgGWRYLDTGQv-----------LIESSCSVERVEETLDTlipmLPEMQYFRFNPVDercgmeLDETDPAV 952
gi 211965692 2420 dtlpagwssllGLGGWETLLa----------qLANCATNTEAVHDLLSDl---LPQSVYFRFNPDIsgd-wpIDETRTQR 2485
gi 156087559 1154 rsgmtcpratqKTVGLDKLLk----------hIIFSATNTEMVHLSLHA----VMGDHYVRINPSMpl--veLDVVVPEV 1217
gi 85001504  1046 nlvklyenfmdHGTSNIKKEnknlsidqlithLSYAVTTSEMTHSALEFt---MPRDVYYRLTPIIln--akIDETNPEV 1120
gi 168024097  786 --------gkgGWRYLDTGQv-----------LIESACSVERAEEALDTllpmLPNLQYFRFNPIDdrcdmeLDETDAAV 846
gi 17544092   435 -------tptvFSTSFQDKLl----------rIIDSATDTEGVHMNVHDm---LPESVYYRFNPYMtya-ygLDEIDQER 493
gi 212511637  416 -----tkksaaKQTSWKEKFy----------nILVSATDTEAVHMILNDl---LPPSVYFRFNPFVtql-lsMDECQPEK 476
gi 159489498  741 --------ekgAHAMLDTGAv-----------LVDAATSPDRADEALSTllplVPGCRYFRFQPVHercameLDDVDPAH 801
gi 157132785  361 --skniieqtsLSSSWKTKFl----------rILDSATDTEATHTMLSDl---LPPGRYFRFNPYLtef-lsMVETRPEK 424
                         570
                  ....*....|....*
Feature 1                        
gi 74734299   727 LDQLQLEGLKYIERN 741
gi 50508404   953 WLKLEAATDEYIQKN 967
gi 211965692 2486 LDVLKCLAERFFLDN 2500
gi 156087559 1218 LQDLKDRTKEFLKQE 1232
gi 85001504  1121 LNKIKDQTKQYLKLS 1135
gi 168024097  847 WSKLETATQEYVDAN 861
gi 17544092   494 LEQMASDAEFYVRRN 508
gi 212511637  477 LDLLKQDALMYIRRN 491
gi 159489498  802 WAALQPSSLFEYFFG 816
gi 157132785  425 IAQLERDTTEYFHRN 439

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