Conserved Protein Domain Family
Pat17_PNPLA8_PNPLA9_like1

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cd07213: Pat17_PNPLA8_PNPLA9_like1 
Patatin-like phospholipase
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes subfamily of PNPLA8 (iPLA2-gamma) and PNPLA9 (iPLA2-beta) like phospholipases from human as well as the Pat17 isozyme from Solanum cardiophyllum.
Statistics
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PSSM-Id: 132852
View PSSM: cd07213
Aligned: 16 rows
Threshold Bit Score: 319.233
Threshold Setting Gi: 154244962
Created: 10-Feb-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 ## #                                     #                             
gi 34495896    6 PYRILSLDGGGLRG---IIALVILDRLDRa------aPGWRDGIHMHAGTSTGALIALGLARGm----TPRQILDQYLER 72
gi 53747908   18 KYRILSLDGGGLRT---LLSIGLLKRIDQl------rPGFLDQVDLVAGTSAGAISALIIAAArepavGLEQARQIWFTP 88
gi 154244962   2 TYRILSLDGGNGGKa--GVFLSTLERRAEvl-----gFSVLRQIDLFAGTSNGAVNALLLAWFddpvlAFEAVLRFDDEV 74
gi 67476083  320 KFRILSLDGCGVRT---LVQLIILKRIVEn------fPHFLDNVDMICSVGASTLPILMTLVGy----DIDTVIKIYEKT 386
gi 16080506    3 KYRIMTFDGGGTLG---ALSLQLLNRLARq------nPKLISRTHVFSGNSIGSFTALALASGr----SPRETLQYFEDE 69
gi 167534961  81 GCRILALDGLGANV---IFHISLLCRLLAq------ePEFDEQPDVYVGSSLGAVTALMLAYGy----SSRQVAQLYEHE 147
gi 67482055  383 KFRVLSLDGGGVKC---IYQCVLLKRIIKe------iPDFFDKIDLITGLSASSLVSVSILLGl----DMEFVIKVMEAV 449
gi 67473852  331 KYRIISLDGGGIKV---LMEVILLQRLSAe------fPDMFVNCNLFCGCSASSVVAVTLSMGy----NLDGLMKLIEHV 397
gi 183230181 356 KFRILSLDGGGIKC---IYQCAILQRILDk------fPDFFEKIDVITGVSASSVPCVAIALGy----DLLSVEKMMEDM 422
gi 108759846   2 PHRVLAMDGTSISGgegYVTAGLLKSLKEtltsrgggQSLLNDVELFSGTSAGSFNAAFFATYenpddAFPKILDFWGEV 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 34495896   73 GPKLFErgaarrlktlngligarydgaerericrdvlggadtlasllrdgGSRGHVLVPAFNLDGDprlp-----qgrRR 147
gi 53747908   89 GLFDSPlsnqlgalvgqsalmps----------enmaralthilgdktlrDLKRKVVIPAFQLDDGdpd------edrRG 152
gi 154244962  75 RESQTPwsiagtvlgnnaamttg----------riktfcdevfgagailaDVKRPVLVNTVQLDNAlsd-------gqRS 137
gi 67476083  387 MVKVFSstshkggvlsykyssk------------fikilatkifqdkklcDLKREVVVPVIKIDSGind-------pnRR 447
gi 16080506   70 ILPAFSisrpggpvfnqqlpysg-----------fikavrnffpadlqliDLRKRIVVPSFKLYSQk----------lDR 128
gi 167534961 148 MEQHLSwlptgfnwrivapfwnshs-------dhakeaflrrcfgqatlrDCQHWVAVAAFDLAGSpdeqelpspqptRH 220
gi 67482055  450 SLETFKneshlagiaghqftnk------------flkvmgerllgnltinDLPRNCCIQSFLIDTGkdd-------tkRT 510
gi 67473852  398 IRYSFKkdsiqsvtnskyindy-------------lrafgevafgnlklhDLPRHVLIPSFLIDSGids-------etRH 457
gi 183230181 423 LCETFSriinkggivghqysnk------------flfvmgdkvfgdltmdKLTRKVCIPSYLTDTGkdd-------pnRT 483
gi 108759846  82 VQMNRKavslgrtamaltgnaalld------ssymrdffcsyfgatlrlgDLKRKVALPSFQLDGHrk--------slRT 147
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                 #                      
gi 34495896  148 WKPKVYHNLptrdg-------sddgAEQAWRVAMRSSAAPTYFPSF-------DGFADGGVFANNPAMCALAqtrda--- 210
gi 53747908  153 WRPRIFHNFpgdt--------fvnlDDYLVDLALRSSSLPIVSPVH-------QGYVDGGLFANNPTMSAVAqaiy---- 213
gi 154244962 138 WLPRTFSNFpgd----------lgrVELIADVVLRTMAMPLQYPIYqsgaqtgPAYVDGAVASNNPALTAITsal----- 202
gi 67476083  448 MIPVLLNNIvlkktntdlddnminnEDLLSDVVLRSFATPGLFTCH-------QSFVDGSFLINQPFAVAKAf------- 513
gi 16080506  129 WTPVLFHNFpgs----------pylNEKVSDVILRSSGAPATQRAY-------QNYVDGYVVATNPSTASIAfavg---- 187
gi 167534961 221 WQPTILTNLprgrga-----hepdlDLPLVEVAMRATATPTYYAIH-------NNHIDGTVFAANPSLSGMArvla---- 284
gi 67482055  511 SKAMLYNNFmke-----------tyDKDLKNICLQSASAPGYFDPL-------NNHVDGSLLVNNPFYLTYPliige--- 569
gi 67473852  458 CKSEAFNNIipg-----------ndTEKVADVCLRSAAAPSYYKPY-------QNYVDGGIVDNVPCGVAWPyliga--- 516
gi 183230181 484 SLAKFYNNFlge-----------evPYSLTEVCIQSAAAPGYFGSV-------NAHLDGGVIINEPCGGVLSyvige--- 542
gi 108759846 148 WKAKIFHNTgpdn--------dsdhNELVVDVLMRSGSPPLSYPVYqgireqgSGYVDGGIFANNPSLVALAqiinnitr 219
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 34495896  211 ---------rlaraIPPESVSMLSLGTGFNASHLdg---------------------dnDWGALQWGRnl------tgLL 254
gi 53747908  214 -----------akaADVRDILVFSLGTGDSVDYLdgy--------------------neNWGWRKWLLdpkqpmafvaAT 262
gi 154244962 203 -----------aqqKALEDIALLSIGTPRNLAGQsrylspg------------lsegsaDWGYRQWLLdpfnplvlvdLF 259
gi 67476083  514 --------------FDPKESVVLSLSGGTYKSYFdae-------------------kytGAGYVQWATcv------tdLF 554
gi 16080506  188 -----------kanVPLDQIAVLSIGTGEAPTRLrrdtrgwgmvsadnirpenlknlppNWGVLLDRSpnep--llpfLQ 254
gi 167534961 285 ----------afpaLSVRDVSVLSLGGAVSRQYAfsa--------------------nlDWGVWQWLPwi------lkLL 328
gi 67482055  570 ----------nglnIAKENIVALSIGSGYPTVPYydie------------------qmgNAGLIQWLIkl------sdFN 615
gi 67473852  517 ----------kgigIDPKDIVCLSLSAGRPTPAHldaq------------------kigNGGLVQWAPvl------adLF 562
gi 183230181 543 ----------yglnVDIKDISVLSIGAGYPAIPYipee------------------elrEAGPVKWTLhm------pdLQ 588
gi 108759846 220 ksrtekvetletepPDLTNILMMSVGNGCMSSYLtphfc----------------kgvaNWGFSKWLLdfhdpmvlvkMM 283
                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                                                                          
gi 34495896  255 MDGVNDVADFQVRq-mLGEGRYLRVSALL-----------AEPIALDDAGRMAQMATIGGQVDLDE 308
gi 53747908  263 IEAGVEAIDFQAKm-lLPRGNYWREDPVV-----------PFNLGNSVEAQIATLDRVVKRHDLSK 316
gi 154244962 260 LQAGAAATSAQCAq--LLGSRFRRVDPPVft--------gPAWDAPETNAVLDTTVDWLNAHGWND 315
gi 67476083  555 KTAGIALADEFAKv--LFGPRAHRLDPKL-----------PCQIDDDSFKDIELLKTIAAKVDLSA 607
gi 16080506  255 MIAGGNGYYESMVsanLLGDRFFRLDPRIpnfsktdpavvPAVIEIANKTNLQPANQFIEKNWGSK 320
gi 167534961 329 NNATADACNNNMQq--VLQDRYLRLQPPAi---------pNLRYDHADRYVCPSFVEKQQTRPLHP 383
gi 67482055  616 IFARKEMGVMESRm--IFKNNYFRLDPHL-----------PIEVDIADAKNIEAIKIMGKEQNLDK 668
gi 67473852  563 MLARRDETVKEGKl--LFGERFLRVDPIL-----------PGQILLDNVEQIEEVKRIAQSYDLEE 615
gi 183230181 589 IESRKSFIEFEGKy--LLGDRFFRLNPKL-----------PYQIDLDYCKDVELIKSMGDNLDLTE 641
gi 108759846 284 LEAGSDAADYQCRm--ILRKKYFRLNPVV-----------DQTLSAANLKQVESTVQSLLSTQSTV 336

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