1OXW


Conserved Protein Domain Family
Pat17_isozyme_like

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cd07214: Pat17_isozyme_like 
Click on image for an interactive view with Cn3D
Patatin-like phospholipase of plants
Pat17 is an isozyme of patatin cloned from Solanum cardiophyllum. Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue, and Nu = nucleophile). Patatin-like phospholipase are included in this group. Members of this family have also been found in vertebrates.
Statistics
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PSSM-Id: 132853
View PSSM: cd07214
Aligned: 7 rows
Threshold Bit Score: 479.625
Threshold Setting Gi: 31615943
Created: 10-Feb-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitenucleophile
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   ## #                                     #                           
1OXW_A        13 GEXVTVLSIDGGGIRGIIPATILEFLEGQLQEXDn-NADARLADYFDVIGGTSTGGLLTAXISTPNEN-------NRPFA 84
gi 7270654    32 GQLVTILSIDGGGIRGIIPGTILAYLESQLQELD--GEEARLVDYFDVISGTSTGGLIVAMLTAQDQSgghsrnsNRPLF 109
gi 167998720  11 GKRLTILSLDGGGVRGIIPATILEELEGYLQGLD--GSEVRIVDYFDLIAGTSTGGLITAMLAAPSRDn-----pKRPMF 83
gi 118484250  14 QGFATILSIDGGGVRGIVPSVVLTALEAKLQKLDvdNKDARIADYFDFVAGTSTGGLMTAMLTTPNAE-------KRPTF 86
gi 116788365  13 GQFITVLSIDGGGVRGIIPAAILEFLEETLQKLD--GPDVSIADYFDVIAGTSTGGLVTAMLAAPNEN-------NRPLF 83
gi 1916805    11 GKKITVLSIDGGGIRGIIPGIILASLESKLQDLD--GPDARIADYFDIIAGTSTGGLITTMLTAPNED-------KKPMY 81
gi 4539656    22 GRLITVLSIDGGGIRGLIPATIITCLETKLQELD--GPDARIADYFDVIAGTSTGALLTSMLAAPDEN-------RRPLF 92
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1OXW_A        85 AAKEIVPFYFEHGPQIFNPsgq---------------------------------------------------------- 106
gi 7270654   110 EAKEIVPFYLKHSPKIFPQprgifcgwg---------------------------------------------------- 137
gi 167998720  84 DASQITQFYRENANKIFQKsrgpfgtf----------------------------------------------------- 110
gi 118484250  87 AAKDIVQFYLDKSQLIFPQtteqyeddelfddeaainsvldearnqiqqyknemrnhiivdplisvlrfllnwgllpnfi 166
gi 116788365  84 AAKDITKFYLDNCPHIFPPttgflqs------------------------------------------------------ 109
gi 1916805    82 QAKDIKDFYLENCPKIFPKesrdny------------------------------------------------------- 106
gi 4539656    93 AAKDLTTFYLENGPNIFPQrkyvliklaavhhrssdvdsiva------------------------adapairvgwltpv 148
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1OXW_A       107 ------ilGPKYDGKYLXQVLqe-kLGETRVHQALTEVVISSFDIKTNKPVIFTKSNLANSpelDAKXYDISYSTAAAPT 179
gi 7270654   138 etivrlvgGPKFNGKYLHDLVeg-fLGDTKLTQSLTNVVIPCFDIKKLQPVIFSSYQAVNNqamNAKLSDICISTSAAPT 216
gi 167998720 111 rknlkslaGPKYKAEGLEDLLlqylDDDIFLSDMLTPVIIPAFDIKLQQPVFFSSSKAKTDvleNAPLLQVCRSTTAAPT 190
gi 118484250 167 rkklrslvFPRYDGVKLHEIIne-eVGQKLLSDALTNVIIPTFDIKLFQPIIFSSLKAQRDkstDARIADVCIGTSAAPY 245
gi 116788365 110 --vfkylnGPKYSGDYLHKILkk-yLGDKRLHQTLTNVVITTYDIQIQQPAIFSTFTAKKDkltDAFLADVCIGSSAAPT 186
gi 1916805   107 --dpihsiGPIYDGEYLRELCnn-lLKDLTVKDTSTDVIIPTFDIKLLLPVIFPSDDAKCNalkNARLADVCISTSAAPV 183
gi 4539656   149 anligtmrGPKYDGVFLHDKIks-lTHDVRIADTVTNIVVPAFDVKYLQPIIFSTYEAKTDalkNAHLSDICISTSAAPT 227
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  #                                                     
1OXW_A       180 YFPPHYFVTNTsng----deyEFNLVDGAVATvADPALLSISVATRLaqkdpaf---asirslNYKKXLLLSLGTGTTSE 252
gi 7270654   217 FFPAHRFTNEDseg----ikhEFNLIDGGIAA-NNPTLCAIAEVTKQiikknpv--mgdisplDFTRFLVISIGTGSIRN 289
gi 167998720 191 YFPPARFTLIDktqep-nrirEFNMIDGGIAV-NNPTYVAITQAIKElrsgglc--sdrvdyyGYDDLLILSLGTGSQVH 266
gi 118484250 246 YFPPYYFKTKV----------DFNLADGGLAA-NNPSLLAVCEVMKEqk-------------mDGRKLLILSLGTGAADQ 301
gi 116788365 187 YLPAYYFQTKDssg----ntrDFNLVDGGIAA-NNPTLLAINQVTKQtlvdcpgfavptskasDTTKFLVLSLGTGQKME 261
gi 1916805   184 LLPAHSFTTEDdkn-----ihTFELIDGGVAA-TNPTLLALTHIRNEiirqnpr--figanltESKSRLVLSLGTGKSEY 255
gi 4539656   228 YFPAHYFTTEPagpgdarpprEYHLVDGGVAA-NNPTMIAMSMLTKEvlrgnpd--fnpgkptEYRNYLVISGPPSLAEK 304
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1OXW_A       253 fdktyt------akeaatWTAVHWXLVIQKXTDAASSYXTDYYLSTAFQALdSKNNYLRVQENALTGttTEXDDASEANX 326
gi 7270654   290 qekynakm--askwglmcWVFESGSTPILDCYSEAIHDMVDYQSSVVFQALrSEKNYLRIDDDSLKGdlGSVDISTEKNM 367
gi 167998720 267 sydaeev----akwgvvdWMVHDGETPLVDMVFNASSDMVDYNLSIIFESQdSSKNYLRITTDSLERsaVKLDDASKSNM 342
gi 118484250 302 sgryvvgdpskwgllrwlWYSENNGSPLIDILTTAPDEMISTYISTIFKYCgWEDNYYRLQAKMELTg-ARMDDASQENL 380
gi 116788365 262 tynatda----akwgligWLSNGGKTPIIDMFSQSSADMVDIHASVVFQAFkSEKNYLRIQVELSGDt-SSTDLSTKKNL 336
gi 1916805   256 kekynadm--tskwrlynWALYNGNSPAVDIFSNASSDMVDSHLSALFKSLdCEDYYLRIQDDTLTGeeSSGHIATEENL 333
gi 4539656   305 ytapqca-----kwgliqWLYEGGFTPIIDIFSHASADMVDIHAAVLFEALhCEKNYLRIQDDSLTGhaSSVDIATKENM 379
                        410       420
                 ....*....|....*....|...
Feature 1                               
1OXW_A       327 ELLVQVGENLLKKPVSEDNPETY 349
gi 7270654   368 EGLVEVGEALLKKRVSRVNLESG 390
gi 167998720 343 DKLVKTAQKLLENPVAQRDFNTG 365
gi 118484250 381 RKLEKIGKDLAAKHDAELEALAQ 403
gi 116788365 337 EDLVELGRKLLDDSVSRVNIETG 359
gi 1916805   334 QRLVEIGTELLEKQESRINLDTG 356
gi 4539656   380 EALIGIGKKLLKKPVARVNIDTG 402

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