Conserved Protein Domain Family
Pat17_PNPLA8_PNPLA9_like2

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cd07215: Pat17_PNPLA8_PNPLA9_like2 
Patatin-like phospholipase of bacteria
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes subfamily of PNPLA8 (iPLA2-gamma) and PNPLA9 (iPLA2-beta) like phospholipases from human as well as the Pat17 isozyme from Solanum cardiophyllum.
Statistics
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PSSM-Id: 132854
View PSSM: cd07215
Aligned: 7 rows
Threshold Bit Score: 521.966
Threshold Setting Gi: 160878290
Created: 10-Feb-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               ## #                                    #                                
gi 124005609   8 RVLSIDGGGIRGVLPGQIMVAIEQQLQkkTNNPEARLADYFDLMAGTSTGGILCAIYLTPdes------grpKYTAEQAV 81
gi 110638409   6 RILSIDGGGIRGIIPATVVVYMENRLQqlTKNPDVRISDYFDFVAGTSTGAILACTYLCPaaag----eavhKFTAADAL 81
gi 88803072    5 RILSIDGGGIRGILPGVVLAQIEDKLQkkTGDPEIKLADMFDFMAGTSTGGILTLAYLTPnkk------nrpKLTAQEAV 78
gi 124008435   6 RILSLDGGGTRGVIPATILVYVEEQLKkkSGNPNTKLADYFDFVAGTSTGGMLACFYLAPgfsddqdktstaKFEATQAL 85
gi 117620702   4 RILSIDGGGIRGILPGQILVSLEEKLKskSHNTSARIADYFDLVAGTSTGAILSAAYVCPnee------grpKYSAKEAV 77
gi 160878290   5 KVLAIDGGGMKGIVSAVLLRSLEDRLQyhSNNYKARIADYFDLIAGTSTGSILTALYLFPner------gesKFSAKEVL 78
gi 209527366   6 RVLSIDGGGLRGLIPAEILVSVEHKIQekTGNPQARLVEYFDLFAGTSAGGIMTCLYLSPdlqd----ptkpRCSAEEAR 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 124005609  82 NLYLENGGDIFKkkmfsf-----ggitneKYPSAPMEEALekyLGNAKLSEMIKECLITSYDIeRSNPHFFKRHkaidnk 156
gi 110638409  82 RFYLEKGNYIFSadiwnkvt-smggflkaRYPHQPLEKVLnvaFKQSKVSELLKPCLITSYDVeRKTPVFFQSHvaqqne 160
gi 88803072   79 NIYLDRGDEIFDaniwqkik-sangvtdeKYDASELEEALqdtFGDLKLSNLLKPCIITSYDIrNGKPHFFKQHkaan-e 156
gi 124008435  86 DFYVQRGNDIFNksrknnwlglrslidatKFSPIMLDNVLlevFGNTRMTELIRPCLVTTYNMkTTSPFFFNSHedtd-k 164
gi 117620702  78 NFYLEDGDEIFDvkfwrsig-tlggtsdeKYSAKELERVLmeaFGETKLSELLKPTCFVSYDVdRREPRIFKQHtaik-n 155
gi 160878290  79 ESYYEYGEYIFKrqkfy-------pfwgpKYTNKYLEEMLlkyFGDATLGSLRKPCLMTSYDTtTRSAVFFNSVtgrkde 151
gi 209527366  82 NFFYQNSRNIFYqpcshaik-nfwgllneKYSHEKFELMMqnfFGDLKLSELLKPSLICSYEIsRRKTHFFTQHdavvsp 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              #                                         
gi 124005609 157 gyDFYMRDVARSTSAAPTYFEPnhatsf--aevkyALIDGGVYVNNPTLCAYAAtrkldfgedkikpTASEMMMVSIGTG 234
gi 110638409 161 alDFYVKDVCRAAASAPLYFEPsridsl--tgisyTLIDGSVYANNPTLCALTEasvlfa-ekgiplTADQFEVVSLGTG 237
gi 88803072  157 iyNFKIKDVARATSAAPTYFETariknd--igtpyPLIDGGVFVNNPALVAYSEvrtmqfenieekiSAKNMLLISIGTG 234
gi 124008435 165 qrDFYVRDVARSTSAAPTYFPPaqinnl-itgeemFNIDGGLFAHDPTMMAYVEcrktkf-pqkehpTAKDMLILSLGTG 242
gi 117620702 156 qkDFLVRELLRGSTAAPTYFEAariystsplkqkfVLVDGGVVANDPALCAYSEavsm------gvsGIKDMIIVSIGTG 229
gi 160878290 152 nrNYLLRDAILASTAAPTYFPPscfhak--dncynCLIDGGVFANNPTLCALIEamkl-----pgcdGIGDTICLSVGNV 224
gi 209527366 161 skDYYVRDVIRATSAAPTFFKVaairsl--gdemyTCVDGGVFANNPTLCAYAEarskl----pdnpTAKDMVILSLGTG 234
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 124005609 235 STKYSYEYe-KAKDWGaiGWIKPLIDIMMKGVSQTVDYQLKqifd--avgkPDQYYRIEPKLvha---vsgMDNAGKENL 308
gi 110638409 238 RNQKVYTYe-QVKDWGglGWLNPLLDVMINGASDAIERELSvqfq--alqaTSQYHRVQPELlda---nqeMDDASPENI 311
gi 88803072  235 SVRKGYEYk-KAKNWGavGWIKPIIEIMMSGNSQTVHYHLKqlfgtlkeedQKDYHRLEPIInta---dteMDNASLENL 310
gi 124008435 243 ASDIQLNQldKSNKWWlgKWATYLPQIVIDGAFDTITTQMRwlfdt-legdQKNYLRVDVPEenpgsfspdLADASPENI 321
gi 117620702 230 KKLKNYSYs-DVKDWGplGWAKPVIDITLEGGPQMTEYYLKqias---tvpNSKYFRIQPELyga---dpaLDNATRENL 302
gi 160878290 225 KNTKSYTYq-KVRRFGllQWAVPIFDILMDASEQTVDYQLKkiyk--svnhQQYYYRMVLNTeee---ipkMDDCSKEAV 298
gi 209527366 235 DVKKGYPYe-QARNWGqfQWLLPLFDIIMTGVAETVDYQMRqiyd--atrsPQQYLRINTTLtdkn--mlpIDKSSDENL 309
                        330       340
                 ....*....|....*....|....*
Feature 1                                 
gi 124005609 309 INLRADGSESALdnedkINKIVDML 333
gi 110638409 312 ERLMHVAAACIEkhkdqLEVIVNRL 336
gi 88803072  311 QKLNEDGLAYISme--kVDAELDRI 333
gi 124008435 322 NKLQEVAQATLEhhrnsLDEFINHL 346
gi 117620702 303 EKLRDAGIRNSEvfdkqLDEIADLL 327
gi 160878290 299 HKLTLYGEKLANrekyrIDELAKRL 323
gi 209527366 310 QAIRRVGQQLAEdyseeLDAIVDLL 334

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