Conserved Protein Domain Family
Pat17_PNPLA8_PNPLA9_like3

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cd07216: Pat17_PNPLA8_PNPLA9_like3 
Patatin-like phospholipase
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes subfamily of PNPLA8 (iPLA2-gamma) and PNPLA9 (iPLA2-beta) like phospholipases from human as well as the Pat17 isozyme from Solanum cardiophyllum.
Statistics
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PSSM-Id: 132855
View PSSM: cd07216
Aligned: 24 rows
Threshold Bit Score: 280.728
Threshold Setting Gi: 85114361
Created: 10-Feb-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 ## #                                                      #             
gi 28950058    16 LRILSLDGGGVRGYSMFLIIQELMHRTfveieg------------raprrsEIPKPCDHFDLIVGTGTGGLIAIMLGRLR 83
gi 189189936  216 KILLSIDGDGIRGLSALLLVESLVNAIcvk-------------------vgQRLDSHQIFDLTGGSSLGGVIAIMLCRLR 276
gi 169613052   44 DLILTLDGGGIRGYSSLLILKQLMHEVaecerrlqrdegpvagstrqkfdeNELLPCHYFDYMYGTSTGGLISVMLARLR 123
gi 46139319     9 LKFLSLDGGGVRGLSSLFILKNVMERVgsk------------------mkrRDLQPYQYFDLIGGTSTGGIIALMLGRMR 70
gi 46139829    13 KRLLALDGGGVRGIMGLVILKELMHRVqkrk-----------------nlpEIPRPSDYFELAGGTSTGGIMGIMLFRLR 75
gi 171687725    8 INLLSFDGGGVRGVTSLLIIHEIMIKIkerh-----------------glaEIPKPCDVFHMIAGTSTGGLIAIMLGRLR 70
gi 116201095   16 LRILCIDGGGIKGYTALLILRRIFRTLsadm-----------------ggnLSPRPCDIFDLIAGTSTGGIIAVMLGRLH 78
gi 169765836   29 LRILSLDGGGVRGLSSLCILREVMQRLsp--------------------egKPRKPCEVFDMIAGTSTGGLCAIMLGRLE 88
gi 170088935  395 LRLLSLDGGGVRGISSLYVLKAVMNKItg---------------------dPNAKPCDYFDMIAGTSTGGLIALMLGRLR 453
gi 116205395  107 LLKSLGNGGGIRGLSELLILNEIMSRLksrf-----------------dskEDLLPADVFHMIGGTSTGGLVALLLGRLR 169
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 28950058    84 lDLETCKELYVRLTRKVFEtdktiagipyrsti-----------------fkatkleeaiqdcvREHTINDREgndgavs 146
gi 189189936  277 mQAYRAREAYKQIAKQVFCnkrdyfkyldphahsrtv----------ddtaleneiknviqqelGNPDEILLDvr----- 341
gi 169613052  124 mTVPQCLDIYRRVGHDLFGhrrnilplatkyhhk---------------plenavreivaefckNHEDCDGDDwhpwspe 188
gi 46139319    71 mSIDDCITEYQRLGSIVFGkrrhgeymfdekv-------------------lvretkavvlkylGNEDAPLLDple---- 127
gi 46139829    76 mTVDDTITEYDRIAKSIFSpkfwgydiswlpgakginnskalvqdsrfdalsmskaidevvekyGLDENDKKLkgnap-- 153
gi 171687725   71 mSTEEALRQYDVCAENIFSskkwanitekfrs--------------------tpminiiqklvaEKDMGEMMRdpa---- 126
gi 116201095   79 mTIDECIEVYERLGKDVFGrpvggqvgrvlrgmtssp---------fydiadlqqsirsvlrarGIESDEPFIere---- 145
gi 169765836   89 mTVDECIEAYNQFMKKIFNvsslrkntrlvwkgcrf-----------gadnieavikelinerlGDSEAPLLNeh----- 152
gi 170088935  454 mSIDECIEAYNTLASKIFSagllnkikdgvdtgary-----------saevleqavkdvikkysGSEDTPMRDpv----- 517
gi 116205395  170 lTVPEAIAHYTSLAETIFTarkpfwkdgrfkasal--------------edsikrvveqtlgnaNEKMDAGNGlr----- 230
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 28950058   147 dtfsplsavsrssaassyprrhasnasvvsfsarspsaqsakpafssrwgnpharlyderenRTKTAVTAMYKgsprNAK 226
gi 189189936  342 ------------------------------------------------------------pdSGDVFAITTQMdi-gTNR 360
gi 169613052  189 ddepvaapaktpes---------------------------slanssmwsatvtkpkkpterMCQSICLTATHsg-qIGE 240
gi 46139319   128 ------------------------------------------------------------ddACKTVVYTLPFknavQQS 147
gi 46139829   154 ---------------------------------------------------------lmhegGARMFCCTTAQn---RAE 173
gi 171687725  127 -----------------------------------------------------------kpaKGKAVVCTMPRkranPKN 147
gi 116201095  146 ------------------------------------------------------------gpGCKVILCATRVe---TGK 162
gi 169765836  153 -------------------------------------------------------------gQCKAFVLAVRQdaanSKG 171
gi 170088935  518 -------------------------------------------------------------dGCKVFVVACRAddlsNRI 536
gi 116205395  231 -------------------------------------------------------------tRCKTFVCAVDTkc-vNKV 248
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                             #           
gi 28950058   227 PAVLRSYDSRkepa-----------pefDCTIWQAGRATCAIGLAFKPiqi-----------gqsIFHDDGAGVFNPSMY 284
gi 189189936  361 AALIRSYQTRritgp---------dldqNMPIWKAMKATSAASRYVQSepgfpq-----rfviekGLVDHGTSKNNPVRD 426
gi 169613052  241 AYLLRSYNHTydqntfppwaltynqgadKLKIWQVTRATSAAPFYFDMlvadiknertgykyekvGFKDGGIRENNPSFA 320
gi 46139319   148 ATALRTYINEdkdp-----------npkKWTIWEAVRATSAATTIFEPfihgp-------pgkqiRYIDAGFGYNNPSDL 209
gi 46139829   174 SMLMRSYKDStiyskskvn-dallkhgdKLTISIAARATSAAPTFFPEvkfpe-------dkpdlVFWDGGLLNNNPIDQ 245
gi 171687725  148 VRRIRSFSPEkdn------------wdkEVKIWEAARATTAATFYFKPqpltvstp--qggskieDYIDAAFGVNNPTNE 213
gi 116201095  163 ADVLRNYKSGhpta-----------enySCRIWEAASATAAAPMYFKSvkfa---------sggeRWCDGAIRRNNPIDE 222
gi 169765836  172 PVHLRSYFNTqqks-----------llpNVKAWEASRATSAAPTYFPPmevstd------kgvkhKLIDAALGANNPVGW 234
gi 170088935  537 ATHLRTYINGnvek-----------swaDYKIWEASRATSAAPTYFPRmkl-----------gdyEYIDGGMGFNNPVLL 594
gi 116205395  249 PTLFRSWAPKkda-------------shNCAIWQAARATTAAPPLFKQivige-------ptgtkAYVDGALGCNNPIRC 308
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 28950058   285 ALDEAvvne------wpgrEVGVFVSIGTGKRPKgsdmnstlwye---gfmgefadarrKLIAKIEGCEKIHEMMkkehl 355
gi 189189936  427 ILFECrklf------ryanDMMIVVSVGTGVGINrnn-------------------eipEMANSVEDRKSEARAWsekfe 481
gi 169613052  321 AYSEHgsmh------gddsEPGLLLSIGTGRPNTendgfaai---------wpgplgkvKLLQKWSEKFAVFKNVlytt- 384
gi 46139319   210 ILQEArslwegdhylslhtDVGVFLSLGTGMGEIvrmdndtvmegl-sakirapvkaieVMKNIVTGTATIHRNVaeqf- 287
gi 46139829   246 LWYTRfelvdp---sepapSISCVISLGTGYVSPgkakpswi----------kvvgvasKVMDFATNTNAKGKDFsrhmk 312
gi 171687725  214 LIKEAvaef------gpsrRLGCLISIGTGTKQErvisraasglrnvcdmwgsifdvgmAMKDMVTNAELVHFDLemrl- 286
gi 116201095  223 ALAELarepe-----wrnrEIGCILSLGTGLARSrsvssn-------------lasflkGALKMLTDAEDTAKVFsasal 284
gi 169765836  235 LWNEVlsvf------gagrPIDCILSIGTGIPKNqvfges-------------vkgaitGLGSAITNTELANILFrtlid 295
gi 170088935  595 LMGEAhlyy------gfarPFGCLVTIGTGMAPNvslapqgvela---tnvvgaavlleGMATLTTNAEHANKLAepl-- 663
gi 116205395  309 VISEAvtef------gvdrLVGCILSIGTGLHRAsalprpigye------rvvpvalmkVVGRLVTDGEEEAGRMeaqfa 376
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
Feature 1                                                                              
gi 28950058   356 v---------------krgvnIENYYRFNVEVGvgef------------gmnEWHRLSDISTNTRRYLQ 397
gi 189189936  482 teh-----------salmernWMKYFRFDVTGLedv-------------pleEWCHEDLIMKKTSEYLA 526
gi 169613052  385 ------------------rgeHRWYKRLNVDKGlqdl------------kldNWEKGSWTNPQTGKTEV 423
gi 46139319   288 ------------------ginSVRYYRFNVDHGleav------------klfDYKKTEDMEVDTLAYLD 326
gi 46139829   313 hl------------nareehkDTKYIRFNPYLKeei-------------gldDYTRMEELKDLARKTMA 356
gi 171687725  287 ------------------rrkPDAYFRFNVPLAadev------------slaQYGKIGKLKSMTAKYLC 325
gi 116201095  285 g---------------rqlaqTCRYYRFNVPHGmedl------------qldEWKATERMKALTTEYLS 326
gi 169765836  296 a--------------yapesrRLKYIRLNVGREiedwpe--------damekENEELAEMDSLTQIDGF 342
gi 170088935  664 -------------------naVGTYYRFNVGKKiqekrwvekvepdlfskwfKGEKPEEIEHFTPENWA 713
gi 116205395  377 hsqlspafggvmwidgsstatIKQSFLQAVRHMlpei------------gknDNLEPDLAVCSVLEEIA 433

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