Conserved Protein Domain Family
Pat17_PNPLA8_PNPLA9_like4

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cd07217: Pat17_PNPLA8_PNPLA9_like4 
Patatin-like phospholipase
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes subfamily of PNPLA8 (iPLA2-gamma) and PNPLA9 (iPLA2-beta) like phospholipases from human as well as the Pat17 isozyme from Solanum cardiophyllum.
Statistics
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PSSM-Id: 132856
View PSSM: cd07217
Aligned: 5 rows
Threshold Bit Score: 540.544
Threshold Setting Gi: 145224873
Created: 11-Feb-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ## #                                     #                              
gi 119900051  15 KKILACDGGGILGLVSVEILTRLETdlrr-eldqPELVLGDWFDFTCGTSTGAIIAACIAaGMSMARIGRFYTESGGQMF 93
gi 87302372    7 KKILACDGGGIRGLISVEILARLEHdlqq-slgqPDLLLGDYFDFICGTSTGGIIAACLSsGMSMSQVRDFYVNNGASMF 85
gi 189500321  13 KKILTLDGGGIRGMITIEVLGKIEQllrkqlnkgPDFVLADYFDFVAGTSTGAIIATCISlGMPVEDIKSFYTKNGRAMF 92
gi 196258131  11 KRILSLDGGGIRGTLSAKILVEIEKalqt-hyqdPLYRLGDFFDLVGGTSTGSILAAGIAkGLSTTDLLSLYELDGVNIF 89
gi 145224873  16 KRILALDGGGIRGALTLGYLGRMEDilrdrhgddPEFRLCDYFDLIGGTSTGSIIATGLAlGFAVDELVDLYRSLGEKVF 95
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 119900051  94 DRaalfkrlrysyddepla---aklrsefdaalghqpsTAEPHATLGSPALRTLLMMVMRNhsTDSPWPVCNNPDARYnd 170
gi 87302372   86 ERakwymklhqnyeaeplalllqqaltcqlngpgsifnESAPPVELGDPRLRGLLMLVLRNhsTDSPWPVCNNPLAKYnq 165
gi 189500321  93 DKafllnrlffnky-------------kheklarklrnTFGAKTTLGSDTLRTVLMMVMRNatTNSPWPISNNPFAKYne 159
gi 196258131  90 QKhwlaqipglrkly----------nqydptnlekkllEVFQETTLGDSTLKCYLSITTKNatTGQTRFFDNNQESYLys 159
gi 145224873  96 DKsrlrlglfgak--------------fpkepllraldTHFGDMTLGGDELRTGLMIMTKRldTGSPWLLHNNPRGKYfd 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                           #                            
gi 119900051 171 sg-----rrdcNLRLPLWQLVRASTAAPTFFPPEVVSFAegtpqayrfVFVDGGVTTYNNPAFLAFQMATarpyaINWPT 245
gi 87302372  166 ld-----rkdcNLHLPLWQLVRASTAAPTFFPPEMVSFApgtdreyqfVFVDGGITTYNNPAYLAFQMATakpyhINWKT 240
gi 189500321 160 svrrntfphdcNLDMPLWKLVRASTAAPTYFPPEVIKVGsk-----efIFVDGGVTMYNNPAFQAFLMATvepynMNWQV 234
gi 196258131 160 ----------eNSQVKLRDIVRASSAAPTFFPPHRFTIGqr-----ayEFIDGGVSLYNNPSFQLFLQAYek-dkLGWEV 223
gi 145224873 162 gdg----gsypNRDLLLRNIVRASTAAPHYFEPETLRIApgv----tgAFVDGGVSPYNNPALQMLMLATcsgygLNWRF 233
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 119900051 246 GTGQLLVVSVGTGTAPRlrpdlqaddlwlldhaknIPGALMNAASAGWDMACRm-----LGECRHGGLLDREIGTMvsap 320
gi 87302372  241 GVDQLLIVSVGTGNAPAarpdlrpeelwlldhaktIPSALMNAAIAGWDMVCRv-----MGECRFGGEVDREFGAMiqep 315
gi 189500321 235 GENNLLVVSVGTGYCANankdlkpsemnilynassIPSALMFAASNEQDFLCRv-----FGDCKAGDLIDREVRTMmgkq 309
gi 196258131 224 GADKLLLVSIGTGFAYEnipvgkaanytaldwapyLVTRLMDDANVGQNQILQlisyqpNRFARKTNKVVKEQTFPhvda 303
gi 145224873 234 GAENLMLVSAGTGHRPLrmtaaqvtdmpavvlaaqSMLSIMADANWLGQAVLQw-----LASSPTSWQIDSEIGDLrddr 308
                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
Feature 1                                                                      
gi 119900051 321 ge---ppnwsGPKLFSYVRYDPDV----SRAGLDALGLSdv--aeADVQRLDAIDRLPAIQR 373
gi 87302372  316 ltpadqsnwtGAKQFAYVRYDPLT----NAEGLIEIGLA------DVSLKEFETMSMEEKQR 367
gi 189500321 310 -------gpvSPKLFTYLRYDVEL----SRQELNVLCLKdi--lpENVQKMDSVDHMEELQR 358
gi 196258131 304 -------eklKDKFLTYYRFNASF----EESRLKALGLSpspqqlKALKKMDCVDQINFLQQ 354
gi 145224873 309 -------lgpGPDLITYQRYEVSLepnwLRDTLDVHIDD------EQCDALYAMDNPKNLTR 357

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