Conserved Protein Domain Family
Pat_PNPLA4

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cd07222: Pat_PNPLA4 
Patatin-like phospholipase domain containing protein 4
PNPLA4, also known as GS2 (gene sequence-2), shows both lipase and transacylation activities. GS2 lipase is expressed in various tissues, predominantly in muscle and adipocytes tissue. It is also expressed in keratinocytes and shows retinyl ester hydrolase, acylglycerol, TG hydrolase, and PLA2 activity. This family includes patatin-like proteins: GS2 from mammals, PNPLA4 (Patatin-like phospholipase domain-containing protein 4), and iPLA2-eta (Calcium-independent phospholipase A2) from Homo sapiens.
Statistics
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PSSM-Id: 132861
View PSSM: cd07222
Aligned: 11 rows
Threshold Bit Score: 378.597
Threshold Setting Gi: 170576049
Created: 12-Feb-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              ## #                            #                                         
gi 116242718   5 NLSFAACGFLGIYHLGAASALCRHGKKLVKdVKAFAGASAGSLVASVLLTAPEKIEECnqft------------------ 66
gi 195539505   5 NLSFASCGFLGIYHLGAAAALYRHGKKLLKvVKDFAGASAGSLAATVLLAVPENIEKCqqft------------------ 66
gi 122692345   5 NLSFAACGFLGIYHLGAASALYNHGRKLLKdVKACAGASAGSLVASVLLTAPQKIEECnkfi------------------ 66
gi 47226790    1 NLSFAAAGFLGIYHVGAAEAFLRHGDKLLGsLGGCAGASAGAMVAAVMVTAPDRLQMTkgfi------------------ 62
gi 157422748   4 NVSFAAAGFLGAYHLGVAKALLKHGDKLLSsLKACAGASAGALVAAMIITAPDKLQHCkeft------------------ 65
gi 147903691   5 NLSFSAAGFLGIYHLGAASALLKHGPKMVKrVKCFAGASAGSLVATVLLTAPDKIKESkefl------------------ 66
gi 126336896   5 NLSFASCGFLGIYHLGVASALYKHGKQLLKvVKAFAGASGGSLVATILLTAPEKIEECneft------------------ 66
gi 34880571    5 HLSFSSCGFLGIYHLGAATVLSKRGRGLLCnVTGFAGASAGALVATLLLTAPERIEACtrft------------------ 66
gi 115534085  96 CLSFGGCGFLGSYQFGAAKMIFDHGKKLMKrVERYSGCSSGSLVAAMFIFNPEKLVFLvsfrktkgrlplfslfllnfrn 175
gi 170576049   9 ALSFSGCGFLGVYHFGVLKGLNRDGKALMKrVKRCAGASAGALAAALWTFQPDDAEKGfndv------------------ 70
gi 71987922   21 ALSFSGSGFLGAYNFGAAKRLMQEKKTIGEkVDRFAGASAGSLVAAILALAPEKLDAAidtl------------------ 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 116242718  67 -------------ykfAEEIRRQSFGAVTPGYDFMARLRSGMESILPPSAHELaqNRLHVSITNAKTR-ENHLVSTFSSR 132
gi 195539505  67 -------------yefAEEVRKLDFGAVTPGYDFMKRLREGIESILPSNAHEIaeNRLYVSVTNVRNG-KNYLFSNFASR 132
gi 122692345  67 -------------ydfAEEIRRQSFGALTPGYDFMARLRSGVESILPTNAHELahGRLHVSITNTRTR-RNCLVSSFPSR 132
gi 47226790   63 -------------selAESVRHRQLGAVVPGSSLMIRLQQLMEVVLPADAHCLanDRLHVSITNVKSG-KNHLVSRFSSR 128
gi 157422748  66 -------------yrfASNVRSQRFGAVTPGYDFMLELREGIEQILPPSAHQLanERLHVSITHSKTH-KNSMVSSFTSR 131
gi 147903691  67 -------------yafSEDVRKQKVGAMTPTYDFMKNLRGGIESILPANAHEIaeHRLYVSITNAKSR-KNCLVSHFASR 132
gi 126336896  67 -------------ynfAEEIRKQYFGAITPGYDFMARLRGGMETILPLNAHEIadTRLYVSITNVKTR-ENNLVSRFPSR 132
gi 34880571   67 -------------fafAEEVRMQALGPLTPGYDFMARLRGGIDEILPQHAHEL--ARERLHVSVTSAVgQNYLVSNFTCR 131
gi 115534085 176 lcilsiseaveeiykmADEVNDKTLGAMSPGFVISDRLRKVVEKFLPDDVAKAn-DLLFVSVTKLKTW-KNELISNFESK 253
gi 170576049  71 -------------ikmATEIHSLRFGALSPDFALHRSLQKIVDFYIPEDISNAq-DKLYISLTDQKKN-VNRLISKFTSR 135
gi 71987922   83 -------------ysmADHVHAQRFGAMTPGYYLNEQLVTIIDDFLPTDIANAq-GRLHISITKLKKW-ENIMINKFDSR 147
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                         #                                              
gi 116242718 133 EDLIKVLLASSFVPIYAGl---KLVEYKGQKWVDGGLTNALPILPvGRTVTISPFSGRLDISPQDkgqldl----yvnia 205
gi 195539505 133 EDLIKVLLASSFVPVYAGm---KPVEYKGEKWVDGGITNGLPILPfGRTITISPFSGRLDICPQDkgrvdl----yvkfa 205
gi 122692345 133 EDLVKVLLASSFLPIYAGl---KPVEYKGQRWVDGGFTNSLPVLPvGRTVTISPFSGRLDISPQDkgrlhf----yvsit 205
gi 47226790  129 DELITALLASSYLPVYAGl---KPVEFRGQKWIDGGFTDSLPVLPvGRTITVSPFAGVQDVCPLHrgqfsa----rfrla 201
gi 157422748 132 EDLIKVLLASCFVPVYAGv---KPVEFQGQKWIDGGFTDSLPILPvGRTITVSPFSGPQDISPEHkgkknl----hlrla 204
gi 147903691 133 EELIKVLLASSYVPLYAGi---KAIDYKGEKWFDGGFTDSLPLLPtGRTITVSPFSGRIDICPQDkthwdl----yvtfa 205
gi 126336896 133 NDLIKVLLASSFVPVYAGl---KAVEYKGQKWVDGGLTNGLPILPvGRTVTVSPFSGRLDICPEDkgqmdi----yinma 205
gi 34880571  132 DNLITVLLASCFIPFYAGl---KPMDCAGQLCVDGGLTNSLPVLPtGRTVTFSPFSGRADVSPRDlgwpgi----yvrva 204
gi 115534085 254 IDLIDCLMASCYHPMYSSglngKAPILRGEAYIDGGYSNMIPEFLdMRTVTVSAFAGDADICPVEksalfnd--fmfaff 331
gi 170576049 136 NYLIDSLLASCYIPFYSGs---SPPVIDGDQYIDGGFTNNLPVFEeLPTITISPFSGSAIIAPNDydslrpfrewrlrvg 212
gi 71987922  148 DHLISCLLASCYIPMYSMgykgVPPIINQEECIDGGMTNNLPTFPdVLTITCSPFSSQADICPDDpstwn------iefg 221
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*....
Feature 1                                                                   
gi 116242718 206 kQDIMLSLANLVRLNQALFPPSkr-----------kMESLYQc---GFDDTVKFLLKEN 250
gi 195539505 206 kQDIMLSLANLVRFNQAMFPPNqe-----------kMESLYQn---GFDDAVHFLLKEN 250
gi 122692345 206 nQEVLLSVANLVRLNRALFPPSrr-----------tMESLYQr---GFDDASRFLVKEN 250
gi 47226790  202 nMSVMLSADNLRRLHQSFFPPPpqlhavlvrgrfqrHREIPEerglGELTASWNLRESV 260
gi 157422748 205 nMSIVFSKENIIRLNQALFPPPlp-----------rLEALEQe---GYQDALRFLKKEK 249
gi 147903691 206 kHSLLLSAANVRRLNQALFPPRqe-----------kMETIFQd---GYKDAVNFLQLKN 250
gi 126336896 206 nQDIMLSRTNLVRLNQALFPPTqr-----------kMKLLYQn---GFDDATKFLLKEN 250
gi 34880571  205 kQDVMVSMANLVRVQQALFPPSrg-----------mMEAIYGm---GMTDTVRFLRRER 249
gi 115534085 332 nQNMKLTLSNMKRVTNALFPPTra-----------iLQEYFNa---GARDAEKLLKDSG 376
gi 170576049 213 tQELKVNVQNMIRGAQALFPPNvd-----------vLKSYYEm---GQRDAMRFLLGVG 257
gi 71987922  222 kQIFKASRRNLYRGARALFPPNrf-----------iLKQYYEm---GISDADIFIKKNL 266

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