Conserved Protein Domain Family
Pat_TGL4-5_like

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cd07230: Pat_TGL4-5_like 
Triacylglycerol lipase 4 and 5
TGL4 and TGL5 are triacylglycerol lipases that are involved in triacylglycerol mobilization and degradation; they are found in lipid particles. Tgl4 is a functional ortholog of mammalian adipose TG lipase (ATGL) and is phosphorylated and activated by cyclin-dependent kinase 1 (Cdk1/Cdc28). TGL4 is 30% homologus to TGL3, whereas TGL5 is 26% homologus to TGL3. This family includes TGL4 (STC1) and TGL5 (STC2) from Saccharomyces cerevisiae.
Statistics
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PSSM-Id: 132868
View PSSM: cd07230
Aligned: 28 rows
Threshold Bit Score: 631.565
Threshold Setting Gi: 74626368
Created: 19-Feb-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 74676509   110 LLYLIRTKWVRNLGNMNNvnLYRHSHTGTKQIIHDYLEEsqavltali--hqsnmndhYLLGILQQTRRn-IGRTALVLS 186
gi 45188104   148 LLYIIRTTWVRDLGNMCNvnLYRHSHVGTKYIIDEYVEEskrslhelv--yqsnldmnYMLGMLVQTRKn-IGRTALVLS 224
gi 50302799   141 LLYLIRTTWVRNRGGMGNvnLYRHSYVGTKHIIEDYVAEssrslqelv--ynsdlddtYLLGMLTQTRKn-IGRTALVLS 217
gi 50555644   152 LLYLVRTTWSRNLGNLGDvkLYHNSFTGTKRLIEDYILEcelalnallaagndkipdqELLTELLNTRKa-FGRTALLLS 230
gi 74626368    68 LLGLLRNSVARDFANMDNsrLYNYAHSGTKKLIDEFIQEvlmcltyl---eetpdlslDEKITEFSRLKltTGNTALILS 144
gi 149246010  151 LLYYVRTSWIRNLGNIGDadLYRHSYVGTKKLIEEYLQEcqlalnylvedggsqlddrYLLGMLIQTRKn-IGRTALVLS 229
gi 150864168   86 LLYYIRTKWIRNLGNMGNinLYRHSLVGTKKLIEEYIDEcqlsldylihdpdvnlddrYLLGMLIQTRKn-IGRTALVLS 164
gi 156845678  172 LVYLIRTNWVRNLGNMGNvnLYRYSHVGTKYLIDDYMNEsrlcieall--hqselddtYLLGMLQQTRRn-IGRTALVLS 248
gi 190346431  134 LLYLIRTKWTRNIGNMGDigLYRHAYVGTKKLIEEYTQEceealeylingsqvdlddrYLLGMLIQTRKn-IGRTALVLS 212
gi 199432551  136 LLYLIRTKWTRNLGNMGDinLYRHSHVGTKTLIEDYIKEcemsldylinnkevslddrYLLGMLIQTRKn-IGRTALVLS 214
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          ## #                        #                                                  
gi 74676509   187 GGSTFGLFHIGVLAALFEsdLMPKVISGSSAGAIVASIFCVHTTQEIPSLLtnvlnmeFNIFNDDNSKSp---------- 256
gi 45188104   225 GGSTFGLFHIGVLSTLFEqeLLPRVISGSSAGAIVASILCVHHKHELVQLIed---vlQKEFNIFEDESqkv-------- 293
gi 50302799   218 GGGTFGLFHIGILVTLFEqeLLPRVISGSSAGAIVASILASRQEHELMGLMde---ilVKDFNIFKDDLems-------- 286
gi 50555644   231 GGGCLGLLHTGVLQALSDtsLLPHVISGSSAGSIMAAGLCIHKDEEHEAFIte---lmERDFDIFEESGn---------- 297
gi 74626368   145 GGGTFGMTHIGVLQSLHEqgLVPKIICGSSAGAIVACAAAVRNKEEQEILLrq---fhTGDLSVFTDPNaa--------- 212
gi 149246010  230 GGSTFGVFHIGVLATLFEanLLPRIISGSSAGSIMASILCSHTNDETVELLqt---iaRRRFNIFDITDydptk---vqg 303
gi 150864168  165 GGSTFGIFHIGVLATLFEanLLPRIISGSSAGSIVASILCCHSNEDTFELLrt---itERDFNIFSDDEspnyk---srs 238
gi 156845678  249 GGGTFGLFHIGVLSTLSEldILPRVISGSSAGAIVASILSVYHKDELPELFer---vlGTDFNIFKDDSlks-------- 317
gi 190346431  213 GGSTFGIFHIGVLITLIEenLLPRIISGSSSGSIMASIMCSHTNEETVELLkn---isEREFRIFEINStldreeptkkg 289
gi 199432551  215 GGSTFGIFHIGVVITLLEssLLPRIISGSSAGSIIASIICSHSNEETVSILnd---imVRRFDIFESGDiekek---dge 288
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 74676509   257 -nENLLIKISRFCQnGTWFNNQPLINTMLsFLGNLTFREAYNKTGKILNITVSpASIYEQPKLLNNLTAPNVLIWSAVCA 335
gi 45188104   294 khESLLMKISRFLKnGTWFDNKHLINTMIgFLGDLTFREAYNRTGKILSITVSpASVYEQPRLLNQLTAPNVLIWSAVCA 373
gi 50302799   287 esENLLIKISRFFKnGTWFDNKNLVSTMInFLGNLTFREAYNRSGKILNITVSpASVHEQPRLLNNLTSPNVLIWSAVCA 366
gi 50555644   298 -eDTVLERVSRMLKhGSLLDNRYMQDTMReLFGDMTFLEAYNRTRRILNVTVSsAGIYEMPRLLNYLTAPNVLIWSAVCA 376
gi 74626368   213 -pPSVIQSVKQYFTrGCVLDISHLERVMKlLIGDFTFQEAYDRSGYILNVTVScGSLFEMPSLLNYITAPNVLVWSAVVA 291
gi 149246010  304 kfRKVLVFLGHLIKyGTIFDIDGLQETMMgFLGDLTFREAYNRTGKILNITVSpASIHEQTRLLNHLTAPNCLIWSAVCA 383
gi 150864168  239 nfGKLLDSFAHFIKyGTLYDMTGLKQTMIgFLGDLTFREAYNRTGKILNITVSpASIHESTRLLNYLTAPNCLVWSAVCA 318
gi 156845678  318 esENFLIKFSRFFKnGTWFDNKHLGRTMIeFLSDLTFREAYNRTGKILNITVSpASLFEQPRLLNNLTAPNVLIWSAVCA 397
gi 190346431  290 nlKNTLECLSHFIKyGTFFDICGLKKTMIdFVGDLTFREAYNRTGKILNITVSpTSIHEQTRLLNYTTAPNCLIWSAVCA 369
gi 199432551  289 syKKVLKNLSHLLKyGTLFDIDSLQNTMIgLVGDLTFREAYNRTGRILNITVSpTSIHEQTRLLNYLTAPNCLIWSAVCA 368
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                #                                        
gi 74676509   336 SCSLPGVFpSTPLFEKDPHTGKIKEWGatnlhlsnmKFMDGSVDNDMPISRLSEMFNVDHIIACQVNIHVFPLLkfsntc 415
gi 45188104   374 SCSLPGVFpSTPIYEKDPKTGETTEWNss-----svKFVDGSVDNDLPIAKLSEMFNVDHIIACQVNIHAFPFLklslsc 448
gi 50302799   367 SCSLPGIFpSSPLYEKDPKTGETKQWNss-----svKFVDGSVDNDLPVSKLSEMFNVDHIIACQVNIHVFPFLklsvsc 441
gi 50555644   377 SCSVPLIFnAYTLLEKEPKTGAIQTWNas-----slRFIDGSVYADVPIARLSEMFNVNHFIVSQVNPHVAPFLkltedk 451
gi 74626368   292 TCSVPFLFkRATLWERDPLTREVSAFCvtd----apLWMDGSVDNDIPHAKLTELFHVNHFIVSQVNFHIVPFImdptsh 367
gi 149246010  384 SCSVPGVFpSSSVYEKNVKTNEIHEWNnde----lmKYMDGSVENDLPITRLLEMFNVDHIIAVQVNPHVVPIMkvsvsn 459
gi 150864168  319 SCSLPGVFpSTTIYEKNPKNNEIHEWNnda----amKYVDGSVDNDLPITRLSEMFNVDHIIAVQVNPHVVPVLkvsvsn 394
gi 156845678  398 SCSLPGIFpSSPLYEKDPKTGETREWIgss----svKFVDGSVDNDLPISRLSEMFNVDHIIACQVNIHVFPILklslsc 473
gi 190346431  370 SCSLPGVFpSTTIYEKIPKTNQIQEWNndt----smKFVDGSMENDLPIARLSEMFNVDHIIACQVNPHVVPMLkisvts 445
gi 199432551  369 SCSLPGVFpSTLIYEKVPNTNGVQEWNnda----smKFVDGSVDNDLPITRLSEMFNADHIIACQVNPHVVPILkvsiss 444
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 74676509   416 vggeiekeitarfrnqvtkifkFFSDETIHFLDILKELEFhpYLMTKLKHLFLQQYSGNVTILPDLsmvgQFHEVLKNPS 495
gi 45188104   449 vggevedefsarlkqnlstvynFVVNEVIHLLELGTELGIarNLFTKMRSVLSQQYSGDITILPDLkmllRIKELLANPT 528
gi 50302799   442 vggeiedefsarfkmalgkvykYMASEVIHALEIATEIGIgtNVLTKLRSVLSQQYSGDITILPEMntllRFNELLSNPT 521
gi 50555644   452 anpdsvd--eiytlklwhnfktLVTDEVMHQLQVLYEFGIfkNLCSKMGGVLSQRYKGDITILPQVhl-sELPGILTNPT 528
gi 74626368   368 nwv----------ercckkaidLAAQEVSLTFRLFAELGIfsVLFTKLQSVITQKYSGDITIIPRLny-rEVNKVIKNPT 436
gi 149246010  460 iggdvendlsyklkhllnnaydFVSCEAIHYLQLLTELDIqkNLSNKLISILSQNYSGDITILPDFkv-sDFAKIFENPK 538
gi 150864168  395 vggeienelsyrfkhllnnvydFVSCEIIHYLQILNVVDVykNLSNKLISVLSQNYSGDITILPDFki-qDFAKIFENPT 473
gi 156845678  474 vggdiedelsakikqgltnvynYMANEAMHFLELGCEVGLakNALTKMRSVLSQQYSGDITILPDLnmlcRINKLLSNPS 553
gi 190346431  446 iggdydnelsykvknlcnnvynFVTSEVVHYLRVLNELNIykNLSSKLISILIQNYSGDITILPDYkv-iDFLKIFENPT 524
gi 199432551  445 iggeveneinykfknflnncydFMTSEIIHYLQILNEMDVykTLSNKLITLISQHYSGDITILPDFks-kDFFKVFKNPT 523
                         410       420       430       440
                  ....*....|....*....|....*....|....*....|..
Feature 1                                                   
gi 74676509   496 QLFLLHqTTLGARATWPKISMIQNNCGQEFALDKAITFLKEK 537
gi 45188104   529 QEFLLReTVNGARATWPKIAIIKNHCGQEFELDRVINYLKGK 570
gi 50302799   522 QEFLLReATNGVRASWPKISIIKNHCGQEFELDKMINYLKGK 563
gi 50555644   529 AAYMKDtNRRGAQATYRKISLIRNHCAIELALDRAIHELKAR 570
gi 74626368   437 PSFLLDaATRGKRGTWTKVPVTRNHCAIEILIAAAYTRLIKR 478
gi 149246010  539 PDFLLDfICRGAKAAWPKISIIHNHCSIEFGLDKAISILRGR 580
gi 150864168  474 PEFLLDfITRGARASWPKVTLIHNHCGVEFALDKAISLLRGR 515
gi 156845678  554 TEFILReTTNGARATWPKISIIQNHCGQEFALERAISFLKGR 595
gi 190346431  525 TDFLLDfVIRGARSSWPKVTVIKNHCGVEFALDKAISLLRGR 566
gi 199432551  524 PAFLLDfIIRGARASWPKVTVIYNHCGVEFALDKAISLLRGR 565

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