Conserved Protein Domain Family
Pat_PLPL

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cd07232: Pat_PLPL 
Patain-like phospholipase
Patatin-like phospholipase. This family consists of various patatin glycoproteins from plants and fungi. The patatin protein accounts for up to 40% of the total soluble protein in potato tubers. Patatin is a storage protein, but it also has the enzymatic activity of a lipid acyl hydrolase, catalyzing the cleavage of fatty acids from membrane lipids. Members of this family have been found also in vertebrates.
Statistics
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PSSM-Id: 132870
View PSSM: cd07232
Aligned: 27 rows
Threshold Bit Score: 472.521
Threshold Setting Gi: 211969479
Created: 18-Feb-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 158517735  330 IEACVKNNFVGIENp---------------rLYSQTYYGTKNLVqny-----vdEVERSIKFLidtk--qltkEQKRVMF 387
gi 74685138   174 LAVCVRANFAGTESv---------------kMYSETFIGTKKAVeah-----ikEVAACLDYVrtat--dvslEEKRAFF 231
gi 169861137  209 LETCIRSNFAAVESp---------------rLYSETYLGTKDLIesy-----fsELEKSIVFIrdsp--hlsiDEKRRFF 266
gi 67588018   391 HCREYDNDLQVIDSgfnisk------dvsdrWNSDWGYYYENKVrknyinqfsfETGTGSVLTfsks---egsLASNSLI 461
gi 220970457 1145 YGEEVERCLEWVANgpv------------ylGGGEGDDTKMNNVes------ncPREEILQTQqd------elQKRYTLV 1200
gi 217405088  334 SRSVVRAYFQQVEEgldwiaeapvlqnlsshQCSEGENGKEAKHmhe----ssrSSSAELTGWaessskhndlLERVTLI 409
gi 116058416  288 LRTVLHRNFAGIDRl---------------lHLRSARVGTKVLVqaf-----ndEIVAAIEYMstt----ddeETAREML 343
gi 213406641  185 LDVCVRSNFAGLDSp---------------mLYSQTYYGTKKLVdef-----ytEVTECLQLLlten--vfdeYESRRVF 242
gi 164655453  195 LTLCVKQGFAGIENl---------------eLYSQSFYGTKQLIery-----yyEVEESLAYLestp--nvsaPVKSAFY 252
gi 71004996   320 LDLCLRNNFAGTENf---------------rLYSETYFGTKYLVesy-----laEIETALEYIektd--kvslETKRSFY 377
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                            ## #                          #                              
gi 158517735  388 KGIc---ANYGRTALCLSGGATFAYYHFGVVKALLEVD--YLPDIITGTSGGALVAALVATRTNDEL-----------KE 451
gi 74685138   232 RAVn---KHYGSSALCLSGGASFGYYHFGVIKAFLEAD--LLPRVITGTSAGGLCAALLCTRTDSEL-----------KE 295
gi 169861137  267 KSAn---ANLGTTALCLSGGASFGYYHFGVVKAFFDAG--LLPRVISGTSAGGLIAAFACCYTDEEL-----------KV 330
gi 67588018   462 HYLellgHSTGKTALCLSGGGALAMYHLGVVKVLLEQD--IMPKIINGTSGGSIVAAILATKSNEEIl----------NE 529
gi 220970457 1201 KRMk---QNMGHTALMLSGGGAQAMYHLGTIKALVESElyEHIHVISGTSGGSISAAMCAIKSPEELlrdvcvktvstDF 1277
gi 217405088  410 RKMk---QNMGRTALMLSGGGAQAMYHLGIIRTLLESKlyQDIKVISGTSGGSIIAAMCATKTPEELy----------NN 476
gi 116058416  344 KVLresyRSLGRTALCLSGGGALAMYHFGVLLTLLRQG--LCPQVISGTSGGSIVAAFLACHSPKDI-----------VT 410
gi 213406641  243 QYFa---RNFGRTALCLSGGASFSFYHLGVVRALLDEN--LLPSVITGTSGGGLVAALLCTRTNEEL-----------KE 306
gi 164655453  253 RAVf---LNFGRSALCLSGGAAFAYYHIGVARALLDAN--LLPNIVSGTSAGGLIAALLCTRTNEEL-----------KE 316
gi 71004996   378 RTVs---KNLGRSALCLSGGASFGYYHIGVVRALLDAN--LLPKVVTGTSAGGLIAALTCTRTDEEL-----------RQ 441
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 158517735  452 LLNPALAHKITACr----------ESFTVWFWRWWKTGARFDSVDWakqcawWCHGSLTFREAYERTGRILNVSCVPSd- 520
gi 74685138   296 LLVPELADKITACs----------DPFTVWFKRFRQTGARFDTIDWarrsmwFTRGSLTFKEAYTKTGRALNISVVPSd- 364
gi 169861137  331 MLVPELADKITACe----------ESVTVWMKRFLKTGARFDSCSF------FTRGSMTFKEAYLRTGRILNISVIPAd- 393
gi 67588018   530 YIKPTVSNMHGHRw---------fPPLLDQIRHFLVKGYMIDPKEFikt-cqTYFKNYTFYEAYKLTGRIVTITVSPTyv 599
gi 220970457 1278 LLTGEMKRKNIRWf----------PELIHMGTYWLKHRLLVDSTEFkrc-ceFYYKDITFEEAFEMTGKHVCITVTASra 1346
gi 217405088  477 ICIPTVVDDFTKTgeqrrenirwfPPVTEMAAYWLKHKLLVDSAYFrrt-cdFYYSDMTFDEAFERTGKHVCITVSASra 555
gi 116058416  411 SIRPDVSTRFGRRw---------fPLPIKMALHFLKHGVLMDEAGFskt-tkAYFGDTTFEEGFAISGLAVSIQVSIG-- 478
gi 213406641  307 IIVPQMASHFESQm----------GNFMDTIVRYVKTGARFSEVAWartcmwFTRGSMTFAEAYERTGRILNITVVPSd- 375
gi 164655453  317 LLVPDLARHITGCd----------DPMHVWIARAWRTGARFDTSKWarkvqfFTRGSLTFREAFERTGKTLNISVVPFe- 385
gi 71004996   442 MLVPALADRITACe----------EPFSVWARRAWATGARFDTVKWaekasfFTMGSMTFKEAYERTGKVLCISVIPAd- 510
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                       #                 
gi 158517735  521 ---pHSPTILCNYLTSPDCVIWSAVLASAAVPGILnPVVLMMKNRDGsl---ePYSFGHKWKDGSLRTDIPIKSLNLHFN 594
gi 74685138   365 ---rHSPTILLNHLTAPNCLIWSAILASAAVPGILnPVVLMAKDRSGni--kpHNLGGSRFKDGSLREDIPLGSLHTQFN 439
gi 169861137  394 ---rHSPTKLLNYITAPDTVIWSTLLASAAVPGILnPVVIMQKLKDGti---vPWNWGSRFKDGSLRVDIPLQGLNLYFN 467
gi 67588018   600 t-peFTEPLVLNCITTPDILLWSAVSASCALPGLM-PVAELFAKENHtnkiirYFPPGMKWMDGSISQDIPHKELSTLFN 677
gi 220970457 1347 sagsGVQRLLLNHISTPNVTLASAVAASCALPGVMaPAKLMIKDGRGkq--vpFEVDGVEWIDGSVQADLPFKRISTLFN 1424
gi 217405088  556 s-ggTAQRLLLNHISTPHVTVASAVAASCALPGVMaPAKLLAKNSSGvl--epFEVDGVEWIDGSVQADLPFQRIATLFA 632
gi 116058416  479 ----SQTGYVLNHLTSPNVLIRTAVCASCALPGLMrPVEILAKDKNGnl--ipFHPPDVKSYDGTITQDIPSARMTELFN 552
gi 213406641  376 ---vHSPPKIINYLTAPNTIIWSAVIASCAVPGILrPVPLMTRSVDGkl---iPYNFGNRWRDGSLRTDIPLAELRTQFN 449
gi 164655453  386 ---qHSPAQLMNHVTAPDCIVWSAVLASAAVPGILnPVCLLQKLPDGsi---iPWSWGNQFRDGSLRVDIPLESLNSMFN 459
gi 71004996   511 ---rHSPVKLLNYVTAPDCVIWSSLLASAAVPGILnPVCLMQKRKGTee--ivPWNWGHRFKDGSLRVDIPLQDLHALFN 585
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 158517735  595 VNFSIVSQVNPHINLFFFssrgsvgqp-vthrkgrgWRGGYLGSATEQYIKLDLTKWLRVLRQLELLPrp-lgQDWSQLW 672
gi 74685138   440 CNFSIVSQTNPHIHLFFFaprgsvgrp-vahrkgkgWRGGFILSALESYIKLDLSKHFKVIRDLDLMPqi-lqSDWSGVF 517
gi 169861137  468 VTNPVVSQVNPHVHLFFFaprgsagkp-vahrkgkgWRGNFLLSAAEQWLKHELTKNFKVIRDLELLPtl-lgQDWSSVF 545
gi 67588018   678 VRQFIVSQVNPHHVPFVSvnpin---------tsiiHPERTFLHNVLNWLTLDIKYRYTKLAKLKLIPklfgkDVSNFWM 748
gi 220970457 1425 ISNYVVAQTNFHVVPFLNka--------------hhPNINTLYWKIFQMCMWDVQNRVLNLSQLGLFPri-fgQDVTKIF 1489
gi 217405088  633 VSSFIVSQTNFHVLPFLNke--------------yhPNQKSLYWQLFQTLEWDIRSRALKLSRLGLFPrl-fgQDISKIF 697
gi 116058416  553 CNNFIVSQVNPHLNFVLHlaeeshgrkqksarsyqrRNAVQKLLRVANFLLLNIKYSIQKLLEVDLLDir-fvRTLQGVL 631
gi 213406641  450 VHYSIVSQTNPHIQIFFFsprgaigrp-vshrkgqgWRGGYLGSALEQYLKFEMIKWLHVVRSLELLPrp-fgQDWSNVF 527
gi 164655453  460 VTHPIVSQVNPHVHLFHFgsrgapgrp-tafrrgrgWRGGFLLSAAEHILKLNLLTNLAILRDLALLPki-lgQDWSSLF 537
gi 71004996   586 VNYPIVSQVNPHVHLFHFgskgspgrp-tahrkgkgWRGGFILSASERILKLNLSMNFKILRDLDLLPai-lgQDWSSVF 663
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
Feature 1                                                                          
gi 158517735  673 LQTFGGTVTIWPKsi--psDFLKILSDPDppRLARMIHEGQQSAFPKVKFIANRLKIERLVERGR 735
gi 74685138   518 LQRFSGDLTLTPRst--igDWFHILSDPDrpQMKRMLRVGERVAWPALGMVRNRMTVERAILRGR 580
gi 169861137  546 LQRFDGAITIWPRt----rDWIRILSDPDrpELERLLACGQIATWPKLHMIENRTRIEKAIFAGR 606
gi 67588018   749 LQEIEGHVTITPRvs--lfDWYRCINHPSydDMLHFITEGERRTWPHVMRIKHMNFLEKTIKDTI 811
gi 220970457 1490 KQKYYGNLTLVPRfttmqiFGLKSLVNPTvaDMEIYLQNGQLAAWPFLRVLKEMLRIEKSIDACL 1554
gi 217405088  698 KQKYYGNLTIVPRfttmqtFGLKSLSNPTikDMEGYLKYGQIAAWPYLNAIRDMIRLEKALDDCL 762
gi 116058416  632 MQDFRGHITILPSlt--wtDYSKILLHPTemDMQRYISRGEQSTWPHIEAIRYTMRIEHALVDGI 694
gi 213406641  528 LQRFEGTVTIWPKtl--lsDFYYILDPPTeqRLGRMILAGKRASFPKLKFISYRMEVEKLIEQGR 590
gi 164655453  538 LQKFQGTVTIYPKsr--lkDWPRILTDPDatELAQIIHSGCYVTYPKQHMISHLVRIERAILRGK 600
gi 71004996   664 LQRFGGAVTILPKtr--awDWVRILSDPDrkELARMMSVGKSVTFPKLHMIENRVRLERAIEQGR 726

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