Conserved Protein Domain Family
THT_oxygenase_C

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cd07257: THT_oxygenase_C 
The C-terminal domain of 2,4,5-trihydroxytoluene (THT) oxygenase
This subfamily contains the C-terminal, catalytic, domain of THT oxygenase. THT oxygenase is an extradiol dioxygenase in the 2,4-dinitrotoluene (DNT) degradation pathway. It catalyzes the conversion of 2,4,5-trihydroxytoluene to an unstable ring fission product, 2,4-dihydroxy-5-methyl-6-oxo-2,4-hexadienoic acid. The native protein was determined to be a dimer by gel filtration. The enzyme belongs to the type I family of extradiol dioxygenases which contains two structurally homologous barrel-shaped domains at the N- and C-terminus of each monomer. The active-site metal is located in the C-terminal barrel. Fe(II) is required for its catalytic activity.
Statistics
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PSSM-Id: 319920
View PSSM: cd07257
Aligned: 9 rows
Threshold Bit Score: 248.407
Threshold Setting Gi: 196187088
Created: 18-Mar-2009
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
putative metalputative active
Feature 1: putative metal binding site [ion binding site], 3 residue positions
Conserved feature residue pattern:H H EClick to see conserved feature residue pattern help
Evidence:
  • Comment:predicted based on homologous proteins in the same family.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #                                                                         #  
gi 115433446 172 KLGHCGLIAK--DFDISFDFYCYHFNFKPSDVVlgp---dgsMMMVFLHVDLGksYSEHHSFFVAqpqgllqpgkPHHAA 246
gi 196187088  70 RLGHVVLSVS--DVSRTVGWWSEKFGVLVSDDIrlp---dgtTAASFLRCDRGsvPTDHHSLIFAtlps--grvgFHHAA 142
gi 9622202   151 RLGHVALLTN--DFKRNFEWMQSRLGLRPTDTMygkd--kndLVGSFMHLSGGgeWTDHHSLALFpdp----sprVHHFS 222
gi 211583408 147 KIGHFGTCVT--DFAKAFDFYTTRFNFKPSDVYst-----gkDITTFLHLDRGseLVDHHCFFFFegp----kphVHHSS 215
gi 159029973 152 RLGHLAIDVK--DFEESYHWYQQTFGLKATDLIyygh--pslRVGGFLRCQRGeeWVDHHTIAMLqgs----hpkVHHTA 223
gi 211591780 162 RWGHYGVTYPdgQYEAIHDWYMRTLSLAVSDVVykg----ddPITCFFHIDRGleYADHHAFFFKkakpt-edlnVAHAA 236
gi 2116732   173 KLGHVVLEVA--DYQATCAWYTHHFGFIPSDVQvlp---dgsPAVTFMRLDLGdtPTDHHTLAIAqgf----maaYSHSA 243
gi 211588501 189 KLGHYGVCTL--NFEALVEFYTKNFNIVPTDFLfiekegkkkNVAVFAHIDRGekHVDHHSFFMSsnp----tshVHHCS 262
gi 256723413 160 KLGHYGFEVNvdKIHEVFDWYSKTFNMKKTDTLfqpq--skkTVMIFIHLDKGkeYVDHHNIFIAgspelkegikAHHTS 237
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                        #                               
gi 115433446 247 FEVNSIESQFIGHDYLSHKGYTSFWGVGRHIEGSQVFDYWFDLDGFLVEHYTDGDLvnedtPIGWIKrDPKDGNNWGPAL 326
gi 196187088 143 FEVIDFDDLVLGGEHLVERGRERVWGVGRHLLGSQVFDYWADPWGNKVEHWTDGDLltadqAMQIQGmDAMTGVQWGPAL 222
gi 9622202   223 FEVEDLDAQGMGNQWLIKHGWKHTWGIGRHIYGSQIFDYWFDPDGNIVEHFTDGDLvrpgcKPGLVA-LDESLFAWGPPM 301
gi 211583408 216 FETHDFDTQVLGHDWLRSKGYQNCWGVGRHIMGSQIFDYWFDPSHFIIEHYVDGDLvndeyPINRSLaSPGNLHVWGPDL 295
gi 159029973 224 FEVQDFDSIKIGHDWLREKGWTPVWGIGRHLLGSQVFDYWQDPFGSLIEHFTDGDLfeqttEPELHAgGLDILYQWGPAI 303
gi 211591780 237 FEVHDFDIQQLGHNYLTSKGHKICWGVGRHVLGSQVFDYWFDPSNFVVEHYADGDLvncetPIAHVQaGPEALSVWGPPL 316
gi 2116732   244 YEVVDADAVGMGQRVLREKGWKHAWGIGRHILGSQIFDYWQDPWGDKHEHYCDGDLftadhPTGVHAvSPEAMSQWGQRM 323
gi 211588501 263 FEVHDLDTQQLGHQWLAEKGYKSVWGVGRHILGSQIFDYWWDTTGNMIEHYADGDLvndqtPIGYGPaGDESLAVWGPEV 342
gi 256723413 238 FEVDDVDSQVVGHHYLRRKGYINVFGVGRHVLGSQIFDYWFDSSGFIVEHYVDGDLvnedsPHADEPaVAATVSSWGPEI 317

                 .
Feature 1         
gi 115433446 327 P 327
gi 196187088 223 P 223
gi 9622202   302 Q 302
gi 211583408 296 P 296
gi 159029973 304 P 304
gi 211591780 317 P 317
gi 2116732   324 P 324
gi 211588501 343 P 343
gi 256723413 318 P 318

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