Conserved Protein Domain Family
THT_Oxygenase_N

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cd07267: THT_Oxygenase_N 
N-terminal domain of 2,4,5-trihydroxytoluene (THT) oxygenase
This subfamily contains the N-terminal, non-catalytic, domain of THT oxygenase. THT oxygenase is an extradiol dioxygenase in the 2,4-dinitrotoluene (DNT) degradation pathway. It catalyzes the conversion of 2,4,5-trihydroxytoluene to an unstable ring fission product, 2,4-dihydroxy-5-methyl-6-oxo-2,4-hexadienoic acid. The native protein was determined to be a dimer by gel filtration. The enzyme belongs to the type I family of extradiol dioxygenases which contains two structurally homologous barrel-shaped domains at the N- and C-terminus of each monomer. The active-site metal is located in the C-terminal barrel. Fe(II) is required for its catalytic activity.
Statistics
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PSSM-Id: 319928
View PSSM: cd07267
Aligned: 16 rows
Threshold Bit Score: 145.5
Threshold Setting Gi: 169629662
Created: 18-Mar-2009
Updated: 18-Aug-2016
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2116732    25 AQALAHLIFERPDLEQADRFLGDFGLQTAQRDa--ATLYMRGTgSAPYCYRIHHAkr--sRFLGFGLALASr-dDLERIA 99
gi 9622202     3 ITDIAYLVNDHTDLEAAERFYTDFGLKVAYRTa-dEIGFRPAL-ARGYCYVARKSsk--pGLRAIAFTASTr-aDLDVAA 77
gi 159029973   4 IADIAYVTYQHPDLDLCERFLRDFGLITAVKTd--TALYMRARgKKQFVYVVEWGke--aKFVSFALEATSe-sVLHELA 78
gi 2116734     4 AQALAHLIFERPDLDEAERFLVDFGLQLAHAKr--TVCTCAAPaPRLTALRVQRAsr--aRFVGFGLTLASr-aDLQPLT 78
gi 115401182   4 VTSLAHVHYAHPDLDKAICFLQDFGLVVEATVd--GKTYLRGYgEQPFVYVAEQStdgrrHFRGGYWVAESr-aDLERAA 80
gi 171691558  12 LVRIAHVYYTHADLDKAREFLADFGFTVADDRg--DTVYFKGYgTEPFVYCATKGae--nEFGGAGFVVESledLELASK 87
gi 169596498 159 LTSTAFVIYNHTNLSAARQFLLDFGLEPARESqdgKVLFFKGYgTEPFIYIARQSss--nTFGGASYEVSSrneLERATK 236
gi 211591780  11 LLKTAFVVYNHANLEKAKSFLLDFGLEVAFERp-gEEIFFKGYgTEPYVYVARKAet--sSFGGAAYVVDSp-eELQKAQ 86
gi 115433446  18 LLGPVCVAYGHPDLERTHTYLTDFGLVESFRKd--EAIYYRGYgVQPVVYIAKKTav--pEFLGVYFEAAGl-aDLEKAT 92
gi 256731587  11 VLRLGHVYYKHKDSAKAEKFLAAFGFIEVKREg--KRIYYRGYgSEPFLYCLEESst--nEFGGAALVVESpedLQKARA 86
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 2116732   100 ALPGASGIEPLe-gPGGGERVRLTDP-SGFLLEAIHGQQA 137
gi 9622202    78 RFPEASPITPIe-rEGGGDKVMLQSP-DGLPFEIVHGIHS 115
gi 159029973  79 TLASASTVETLf-tPGGGQRIKIIDP-NGFNLELVYGIEE 116
gi 2116734    79 ALPGASPIETLh-tPGGGECVQLTDP-SGFRFEAVFGQQS 116
gi 115401182  81 ANPAAVSEIEEssaPGGGEVVRLRDP-WGFLVGFVHGQRL 119
gi 171691558  88 TLPKATPVHDSd-aPGGGKRVTFRDPvDDFPFHLVYGQTP 126
gi 169596498 237 TIPGCSAIEQLa-aPGGGEYVKLIDP-AGHIVLLVHGQTK 274
gi 211591780  87 KIPGASALVQLegqVGGGQQVTLTDP-FGHQVHLIWGWQE 125
gi 115433446  93 KIPGAGAILDF---AAGGKVVQIIDP-SGIPFHVVCGLEK 128
gi 256731587  87 IFPDATDIYDLdeaPGGGRCVTVKDPiDGWPLHLVYGQTP 126
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