Conserved Protein Domain Family
PX_SNX20_21_like

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cd07279: PX_SNX20_21_like 
The phosphoinositide binding Phox Homology domain of Sorting Nexins 20 and 21
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily consists of SNX20, SNX21, and similar proteins. SNX20 interacts with P-Selectin glycoprotein ligand-1 (PSGL-1), a surface-expressed mucin that acts as a ligand for the selectin family of adhesion proteins. It may function in the sorting and cycling of PSGL-1 into endosomes. SNX21, also called SNX-L, is distinctly and highly-expressed in fetal liver and may be involved in protein sorting and degradation during embryonic liver development.
Statistics
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PSSM-Id: 132812
View PSSM: cd07279
Aligned: 8 rows
Threshold Bit Score: 169.816
Threshold Setting Gi: 212515253
Created: 14-Jan-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                              ###                       
gi 33504571   76 LLFEIASARIEERKv-------------SKFVVYQIIVIqtg-sfDNNKAVLERRYSDFAKLQKALLKTFREEIEDVEFP 141
gi 157127156  57 LCFEIPHARVLPPAltsvdgcgasassgKKYVVYDVSVRqdtsepDPNPTSIERRYTHFLKLYDGLRKEHPLLVQTINFP 136
gi 212515253   3 TKFDIVSARTVDDKen-----------qKKYVVYDIKIYhqgsdnDTQAIFVERRYSDFLELFLNLRQNYPQLTTKISFP 71
gi 196003264 106 LKFESVAARTVTEGk-------------KKFVLYALALVrtd-dtDEIPPIIERRYSQFATLHKALSSSHPQIVKKFNFP 171
gi 19920598   71 LRFDILLAHIMPPDged--------vkiKRFVVYELTVKqdgateDTQPAKIERRYTDFRELYLGLKRQHPAEMANKYFP 142
gi 60729601  121 LLFEVTSANVVKDPp-------------SKYVLYTLAVIgpg-ppDCQPAQISRRYSDFERLHRNLQRQFRGPMAAISFP 186
gi 219409848   3 IVFEVMNARTVKEGh-------------SKYVVYQVHVVksp-glDTSPAFIEKRYSQFDKLNQCLRKRFPHLLESVAFP 68
gi 91082913   12 LVFEILSARICDEGe------------eRKHVVYTLQVRhisgndDLSPSVVERRYTHFLNLYSALKKEHPNLMTNVTFP 79
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
Feature 1        ##             #                                         
gi 33504571  142 RKHLTGNFAEEMICERRRALQEYLGLLYAIrcvr-----------rsrEFLDFLTRP 187
gi 157127156 137 KKVLVGNFSTELIGERSMAFESFLDYIVSVpslr-----------dsdHFLEFLQGD 182
gi 212515253  72 RKVLIGNFSSDLIQTRLSSLENFLRIISENvtll-----------eslGFKMFLIGN 117
gi 196003264 172 RKVAMGNFSQSTIIKRSTAFQDYLNLILKSsnydrgdsvpyasvrytkAFEEFLYIS 228
gi 19920598  143 AKVLMGNFKSELIGERSAAFEAFLTYVASQamlr-----------dseYFLRFLQHD 188
gi 60729601  187 RKRLRRNFTAETIARRSRAFEQFLGHLQAVpelr-----------hapDLQDFFVLP 232
gi 219409848  69 KKTLTGNFKTQTIAERSRAFEQYLQHLHSIpqva-----------tchEFLDFFYLE 114
gi 91082913   80 KKVIIGNFDNELISTRSTGFESLLRHICTEsklr-----------askALVEFLQGV 125

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