Conserved Protein Domain Family
PX_SNX18

?
cd07286: PX_SNX18 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 18
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX18, like SNX9, contains an N-terminal Src Homology 3 (SH3) domain, a PX domain, and a C-terminal Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature. The PX-BAR structural unit helps determine specific membrane localization. SNX18 is localized to peripheral endosomal structures, and acts in a trafficking pathway that is clathrin-independent but relies on AP-1 and PACS1.
Statistics
?
PSSM-Id: 132819
View PSSM: cd07286
Aligned: 4 rows
Threshold Bit Score: 290.417
Threshold Setting Gi: 147907046
Created: 11-Feb-2009
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:based on the binding of phosphatidylinositol-3-phosphate to human SNX9
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                            ###                       #             #   
gi 215273942 276 FQCTIDDPTkqtKFKGMKSYISYKLVPThtQVPVHRRYKHFDWLYARLAEKFPvISVPHLPEKQATGRFEEDFISKRRKG 355
gi 118103782 252 FQCSIEDPTkqtKFKGMKSYIAYKLVPShtGQQVHRRYKHFDWLYGRLAEKFPvISVPHLPEKQATGRFEEDFISKRRKG 331
gi 147907046 245 FRCNIDEPTkqtKFKGMKSYISYRLLPShtGLQVHRRYKHFDWLYARLLYKFPvISVPRIPEKQATGRFEEDFISKRRKG 324
gi 153792003 221 FSCTIDDPTkqtKFKGMKSYISYKLTPThtQSQVNRRYKHFDWLYARLVEKFPvISVPHIPEKQATGRFEEDFISKRRRG 300
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
Feature 1                                                        
gi 215273942 356 LIWWMNHMASHPVLAQCDVFQHFLTCPsstDEKAWKQGKRKAEKDEMV 403
gi 118103782 332 LAWWMDHMCSHPVLAQCDAFQHFLTCPs-tDEKAWKQGKRKAEKDEMV 378
gi 147907046 325 LVWWMDHMCSHPVLARCDAFQHFLTCT---DDKAWKQGKRKCERDDMV 369
gi 153792003 301 LIWWMNHMTSHPVLARCDVFQHFLSCSs-tDEKAWKQGKRKAEKDEMV 347

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap