Conserved Protein Domain Family
PriL_PriS_Eukaryotic

?
cd07322: PriL_PriS_Eukaryotic 
Eukaryotic core primase: Large subunit, PriL
Primases synthesize the RNA primers required for DNA replication. Primases are grouped into two classes, bacteria/bacteriophage and archaeal/eukaryotic. The proteins in the two classes differ in structure and the replication apparatus components. Archaeal/eukaryotic core primase is a heterodimeric enzyme consisting of a small catalytic subunit (PriS) and a large subunit (PriL). In eukaryotic organisms, a heterotetrameric enzyme formed by DNA polymerase alpha, the B subunit and two primase subunits has primase activity. Although the catalytic activity resides within PriS, the PriL subunit is essential for primase function as disruption of the PriL gene in yeast is lethal. PriL is composed of two structural domains. Several functions have been proposed for PriL such as stabilization of the PriS, involvement in synthesis initiation, improvement of primase processivity, determination of product size and transfer of the products to DNA polymerase alpha.
Statistics
?
PSSM-Id: 143474
View PSSM: cd07322
Aligned: 48 rows
Threshold Bit Score: 306.496
Threshold Setting Gi: 215501499
Created: 3-Mar-2009
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
PriL_PriS
Feature 1:PriL_PriS interface [polypeptide binding site]
Evidence:
  • Comment:Archaeal/eukaryotic primases form a heterodimer consisting of a small catalytic subunit (PriS) and a large subunit (PriL).

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 34395782   40 FYSTPPTADITIEQ------FEQWAIDRLRILAELEACsfrnktpaetashmkpl------------------------- 88
gi 74858176   22 LYINVPDFNCNMSQ------IENYCIDRFNLLNEVSKLkdtlsaqksntskdne-------------------------- 69
gi 71747016   34 MYTCNPAGNKTLFE------LEAIVVKRLELLGWIDQLlnspnmknlsqlldevgahlplerrkqstqpaadhvvlgrds 107
gi 154331565  34 MYEQKPHGNSTLFE------LEAMVAKRMELLAWIDQRmnspqaksfesvldtiiarlpeerrsstatdasrgkmvslgy 107
gi 118381080  20 ILLENAIDNSNIQGfqtiqeIVQLAMQRMKMIKEIIQEeprfnlidplskpnynrirv---------------------- 77
gi 123396281  22 LYNVEPSANISISD------MIEFAYLRSQVHQEINDLengiekrdqfekimikygipik-------------------- 75
gi 169806208  15 FYDKLNIIMPGKIPye---tFKSYSIDRIKLLRKIESGtdn--------------------------------------- 52
gi 74621219   17 FYTALSKSSVDYET------FRKCAERRIEMLRCEEGDgiis-------------------------------------- 52
gi 123423419  22 TYETIPSMQLSFEE------ILSIAEKRFQFHRLLEFEesqetvefskirsl---------------------------- 67
gi 74889439   56 VYQTVGTISWSNVYda---lEKRWSLYRLMYNEETNSEkitqels----------------------------------- 97
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 34395782   89 --------------ldkylpldtnsssssqlFAQRQKDHYSHFILRLAFASt-------EDLRRRFTRVETMLFRMRLna 147
gi 74858176   70 ----------------dfrnlssrflgstkdEQFKIKDELSHYCLRLAMCD--------STDRNWFITTESILLANRLn- 124
gi 71747016  108 vdgstsdtpgsraprhssrsltamlstqnvfVFEGDEDLLSHRLARFVFCMs-------EKWRKWFVRTEEMLLRARLrl 180
gi 154331565 108 detasgsgsgrrsssrgstggaagssqaasiVFAPEEDVTSHLLCRFAFCMs-------ERWRDWLVRTERVLLMARIkm 180
gi 118381080  78 -----------ankfitvaipksdpeyelknKQQYAKEVLSHFLCKMAYAFya-----ePETWLEFARAEAFILMDKLk- 140
gi 123396281  76 ----------rprdgvfnpsnsvipnfecgqITKQFLDEMSFYAFMVISLRt-------DETQHKFVKVESEIFKARI-- 136
gi 169806208  53 ----------------------------iftIDSLYKDLISHFALRLIVIQs-------HVTLQWFVTMETKLFKYRInq 97
gi 74621219   53 ----------------------------kclSTSIEEDILSHFFCRVVCSQd-------AWNAVWFVNAEVNLLRLRIsk 97
gi 123423419  68 -----------------sleydiplkeasetDGEQTKDQISFFSLLMIYTEt-------EEQQTKLADYESRLFFYRLkl 123
gi 74889439   98 -------------------------elvkvnENNRWDDEVSYALFFASFCDfqnnvrcnKKLENWFVLCEMRVYLARIet 152
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                            ##  #   #   
gi 34395782  148 ------ddGRERAAFINSLNLDWETVsdeekrelaaelaatasfggykkgqqqqqqyeedqQTWCKVSWLRVPELVEQRr 221
gi 74858176  125 -------qSDLTTIISSFKGFGWSPVsqeeyeqyqddlgy-------lvkkanlnlnevnnTHFYKLPFQDIPKLIDTKk 190
gi 71747016  181 ql--akceADFLPNLMRLNGLPCDALtddqqqdprlqkyii------yhtavqgkgqlrraDEYFLVPLSLATRLIKARs 252
gi 154331565 181 ev--aknpLTFLVDLMKHNGLPCAPLsdvqladpllqeyley----rrvkadsaresegraENYYAVPLSLATRLIKKRh 254
gi 118381080 141 -------sGQHHANFFSDENLKIKTIsdelfkqafpkieatfssikikkndseveqinikkDNFKMFKFIDHPSMLSNNd 213
gi 123396281 137 --------RYALDQHAKLHEEDGQPFkfpqelleiphp------------klnldqhynrkTKMYTIPFELVFEFINVYt 196
gi 169806208  98 mntyefnhFYNTKFISKFNNLTESTItnnikkekqvqkis--------kygqydpdfdippINYEFIHFSKCSHTMSKR- 168
gi 74621219   98 -------nPGGARAFFLSEVLPHMDGartqdgvlvlgmr----------srynpdvvasaaSSEVLVHFTKVIDLIGKRa 160
gi 123423419 124 sy---sspKQIPDFFWPSKELKDQIIns-------------------------------qkDDYYCVPFEQIFDQIDVRt 169
gi 74889439  153 ------lsLEALSQFLVKFGYHPQQItvdeegfl--------------------kqqnyfqGPFIKVPFFEAGKNLKNRr 206
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         ######                                                                         
gi 34395782  222 vFLRQGYAYVPARE--QQAMVVSEFSSRLERQLELTara---lpRLDEDDRLTPILAHLSKnfitpdasyvgtssaissa 296
gi 74858176  191 aIIIKGEAYIYISN--FKDMVIHTFKNYMNFALDRIkad--ktrIKEEFPELIQFFTTLPKagdgtnkpql------gnt 260
gi 71747016  253 vLCLRGHAILHRDQ--VQEVFVTMFLSKLNKGLHEAylarmklsSHEDHDERETVMKMLDAfleyfiedtmnev-qeasa 329
gi 154331565 255 vLCRAGQAILFRDQ--VQEVFLAVFCARLNRGLHSAylarvkqqALEEETAKSTVMAMLDAflqqfvsdpvdsl-qegva 331
gi 118381080 214 vVLHKGYIIIYKES--TSKIVQNIFIERLLDEMRLLqlkf-qnnGSKLDDDRLSFLKDLHKaeifndstqf-------ns 283
gi 123396281 197 cRLSDGFVEVSPKE--LDSFFTKLYQIYLEKRIARYr------aAKIHSSDLTKIAISLFDeragsfvaks------rqn 262
gi 169806208 169 -KIKLVQGYLPSTPetQKDFVITEFNNFIEREMHKLs------nNSIRDERLIRLHSDLFIscknssd------------ 229
gi 74621219  161 vVPEGGYLRLNDEG--IGSLLVSEFRRYLSNRMDELve----isSNEPDERMRVLSTSLLAnptgye------------- 221
gi 123423419 170 iDVENGIAKLTTDH--VYQIVQKNFNDFLVKKLHSAef-----sNLDFISYLRSEFKRVTKseddqi------------- 229
gi 74889439  207 lLLYHGSAFIEKNE--VIDMVIMKNREKLQERVKNNyis---paLGKVPYELKPILEYYQTlvmkitglktat--tgfhv 279
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 34395782  297 disarniDTLVNNHHFPACMSHLHRTLRRDAHLKHygrlqYTLFLKGIGLNLEECLLFWRQSFNkit--------ddtFN 368
gi 74858176  261 khirkedVSPLSKTSFPMCMRVMYDSLVETSSLKYegrlqFGTFLKGIGLPYDEAINFWRIAFSkrvs-------ntdFD 333
gi 71747016  330 gavgagdVRHLAQTHFPLCMRQVDEHLRREGHLKHqgrftYGLFLKAIGLSMQDSMVLFSSLMTlkagaagkgdpesfAK 409
gi 154331565 332 gtvragdVQQLAQTHFPPCMRAVDSHLRREGHLKHhgrftYGLFLKAIGLSLEDSLELFATLMKvkggg----sveafSK 407
gi 118381080 284 tqihhyeLDRLAKRDMPACMTYLMYGLNQKLHLKHfgrlqLGLFLKGAGLSLNEALTFWQKKFSkts--------addFK 355
gi 123396281 263 fsrvtlqDMDAVIRSFPPCMRSMYDALKKNHKLFHsgrlqFGLYLKGIGLTMDESLKFWRSEFTkais-------lddFE 335
gi 169806208 230 kgtpnisIKNIYQENFPLCMKLLIGKLEREKHLKHndrnqLSLFLKDLGVPIEDTISYMQSHLYq------------mKN 297
gi 74621219  222 --rgtnfSLESSERYFPPCMQDIMERLRKNKHLKYndrqtLCLFLKDCGMSVEDGIAFFRGSFKapr---------evFD 290
gi 123423419 230 --ltldnYEELCHRSAPPCIYRILDSLKKSSQLSVkgrfeLCMFLKGLGFDYFSQYIFWKKHFFspkn-------pdlFD 300
gi 74889439  280 etcsksfLKESGIELFPPCMYSIYRKFLHDKHLKHfgllqLIMFLKGVGLSMEDCKKFFEEIEP--------------LS 345
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 34395782  369 KEYRYNVRHTYGDVGGdsnrrGGGYSPYSCQKILTeh-ppGPGEAHGCPYRHFNMENLqtllqqgmg----------vtd 437
gi 74858176  334 KEYLYNIRHNYGLEGK-----STNYSPYSCPRIISksphnGDKLVHGCPYIQSLERLEqklldlg--------------i 394
gi 71747016  410 TSYGYNIRHNYGMEGK-----KTSYSSLSCTSLLGlppvvDKFDCHGCPFRFKNESAFrgvllkeqlhplgkghssirla 484
gi 154331565 408 TAYGYNVRHNYGMEGK-----KTSYSSASCATILGlppvvDQHDCHGCPFRFRDEGALrtmlgketpnpkgrtypsvrpa 482
gi 118381080 356 KKYDYNIRHNYGKLGK-----QLDYTPMSCQKIIGfq--pLKDEFHGCPYKTMESQQLkdflklsyn-----------it 417
gi 123396281 336 KHYAYNIRHTYGKEGA-----GKNLSAYSCAGILRqp-apASGQTHGCPFKMMQMPQLealikeinp-----------ei 398
gi 169806208 298 KELVYNIRHNYGLEGK-----KANYSCFSCQKIFSl---sSEPNFTGCPFINNISLAE---------------------- 347
gi 74621219  291 KEYLYSIRHNYGLEGK-----RANYSCFSCSKIANm---tNEERRSSCPFVGDPEHIRerikd----------------- 345
gi 123423419 301 KQIVPSLKYIYCIDGN-----KKKYQPHSCIALIGqdnpeNPYQVQGCPFRYMMRAELkmyikkmgr-----------ki 364
gi 74889439  346 KERIYNIEHSYGRAGA-----KIDYTGYGCNNLISqi--rNAGDCNGCMMTTKSFKQEdiednllslmelk--gideikr 416
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*....
Feature 1                                                                   
gi 34395782  438 rgvlngVKEDKEKQKFHMACNRVFEHLHKeelkkakdegimtaaqletIVHPNEYFKRS 496
gi 74858176  395 ddfqriQILEATKTSPNVACTKHFNFLHPnntl------------tklINHPNQFYDQS 441
gi 71747016  485 asdiedIIQDCKGQHYTRACYKYFMATHPgarr------------dtlFRSPHEYYVTS 531
gi 154331565 483 pgdiedIVSDCKSQHYTRACYKYFMAIHPdarr------------dtlFRSPYEYFSVS 529
gi 118381080 418 deqfvqVNIFKNQKQYQLACKEVFKVLNDtrdktkeq---fypyfdkvGNHPNAYFEQS 473
gi 123396281 399 agkymaEINAKARENPGSACAILFNSLHPdhel-----------detgVFHPNVYFNES 446
gi 169806208 348 ------QFINIESADPLTNCKKYFVTQLQdknis---------snnllIRSPAEFYKAL 391
gi 74621219  346 --vgadIEDIMGEGPFNGKCTRLLEKLTCrkq-------------srlVATPVRYYLEC 389
gi 123423419 365 knevisKICEQVPEHPQIACKLFFDGMFEedpl-----------dyagMSHPIQFFKEA 412
gi 74889439  417 eeilqsVIDLIISYQPTHACTKFLYLSQNkpfdd----------eiqdIIHPNTYFLRA 465

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap