Conserved Protein Domain Family
M48B_HtpX_like

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cd07327: M48B_HtpX_like 
HtpX-like membrane-bound metallopeptidase
This family contains peptidase M48 subfamily B, also known as HtpX, which consists of proteins smaller than Ste24p, with homology restricted to the C-terminal half of Ste24p. HtpX, an integral membrane (IM) metallopeptidase, is widespread in bacteria and archaea, and plays a central role in protein quality control by preventing the accumulation of misfolded proteins in the membrane. Its expression is controlled by the Cpx stress response system, which senses abnormal membrane proteins. HtpX participates in the proteolytic quality control of these misfolded proteins by undergoing self-degradation and eliminating them by collaborating with FtsH, a membrane-bound and ATP-dependent protease. HtpX contains the zinc binding motif (HEXXH), has an FtsH-like topology, and is capable of introducing endoproteolytic cleavages into SecY (also an FtsH substrate). However, HtpX does not have an ATPase activity and will only act against cytoplasmic regions of a target membrane protein. Thus, HtpX and FtsH have overlapping and/or complementary functions, which are especially important at high temperature; in E. coli and Xylella fastidiosa, HtpX is heat-inducible, while in Streptococcus gordonii it is not. Mutation studies of HtpX-like M48 metalloprotease from Leptospira interrogans (LA4131) has been shown to result in altered expression of a subset of metal toxicity and stress response genes.
Statistics
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PSSM-Id: 320686
View PSSM: cd07327
Aligned: 30 rows
Threshold Bit Score: 138.926
Threshold Setting Gi: 805374630
Created: 14-Jul-2008
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Zn binding siteputative active
Feature 1:Zn binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 24212639   47 SYWFSDSIVLnwynari------------------------vseeeapELHYIVEKLARQAGIP---KPRVAIVPTm-vP 98
gi 122423522  50 SLLMSKWMALrsvggevie---------------------qprnererWLVNTVATQARQAGIA---MPQVAIYHAp-dI 104
gi 805374630  61 IYVVSLVIAIspfgewilrlhsgc-----------rkikrkdqidyiePIFKEVLKQARELDPHiseDVKLYIRNTq-dV 128
gi 150421574  48 VQWLFSPYIInmvyrtr------------------------eplpgeeWIVAEVEQLARRSGLK---PPKVVVSEMn-mP 99
gi 575416314  44 LYRWSERVVLrlheare------------------------iteaeapDCLAITRALALRAGIP---APRLYAVDTl-tP 95
gi 517827260  44 VFWKADTLVLrankaqevd--------------------enhpspmvrAFVKECRDLSSSAGMI---CPRLFIMDAh-qP 99
gi 542111121  50 GYWNSDKIAIramharp------------------------vseleqpAMYRIVRELSTEARQP---MPRLYVSPTq-aP 101
gi 296888791  53 AYWCSSKLAIiamrate------------------------vseeeepVLYAMVRDLSARIGKP---MPRVMVSPYe-sP 104
gi 815099558  54 SYWNSATIALrsmnayq------------------------vtreerpDLYRIVEELAARAKQP---VPSIWIAPTa-tP 105
gi 744452758  55 IGLPSLYWVVtttyryfsavptyinqrnirvinpflldekglyskeeqWLCSTIRDLAIAEKIPk--PPVVGIELCddnA 132
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          #   #                                         
gi 24212639   99 NAFATGrsprnAVVAVTEGLLqllnkDELEGVIAHEISHIKNrDTLIQTIAAvlagaimilvdfarwslwfgaydder-- 176
gi 122423522 105 NAFATGarrdaSLVAVSTGLLqnmspDEAEAVIAHEISHIANgDMVTMTLIQgvvntfvifisrilaqlaagfmggnrde 184
gi 805374630 129 NAFAIGr----KTICITDGLLka-pvEQIKATIAHEIGHIAHrDTDWILVISvgnfivtfvyiivrvfgkmmstffellp 203
gi 150421574 100 NAFAYGspiagSYVAVTRGLLrllpkDEVRAVLAHEVGHLKHrDVTVILALSlipiaafligrtlvwagilgggggerr- 178
gi 575416314  96 NAFAIGrssqhASLVITEGLLqrldqDEWTSVIAHEVAHIKHrDILVMTMTAalagilsrpfasvipqwpfladpipag- 174
gi 517827260 100 NAFAVGrdalhASIAVTNGLLmtlnrAETRAVIAHELAHVKRgDSLSMGIASavsgllnrltwiffaplg---------- 169
gi 542111121 102 NAFATGrnpknAAVCCTEGILrlldeRELRGVLGHELMHVYNrDILTSSVAGalagvitslaqfllifggdrdrg----- 176
gi 296888791 105 NAFATGrserhASICCTRGLInilneRELRSVIGHELTHIYNhDILASSLATatatvltyvgswlvyvgdtyrrdir--- 181
gi 815099558 106 NAFATGrdpnhAAVCCTEGILeilgeRELRGVLGHELMHVYNrDILTSSVAAgiagligsvaqfllffgggsrrdgeg-- 183
gi 744452758 133 NAYAVGatrsySMVVATTGLMkhlnrEQVAAVMAHEIGHIANlDTMAKTLLSaswsgvmmvlfapmllvvwylgrfvall 212
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                            #                           
gi 24212639  177 ----------------------dsgsviglilaiilaplaatliqlaISRSREYLADETGARISgkphaLASALMKIeea 234
gi 122423522 185 ge---------------esngnpliyfavatvlelvfgilasiitmwFSRHREFHADAGSAKLVgr-ekMIAALQRLkts 248
gi 805374630 204 ssdnltd-----ggvkvagmvqsyitdaiiialmwawtkigillvmkSNRDNEFEADKFAFNLS-----YGNALCELlds 273
gi 150421574 179 ---------------------gnpmalvavgaallaagmvfqlivshFNRLREYYADAHSALVTgsprsLQRALARIhaa 237
gi 575416314 175 --------------------skdglrtaagalasvivtplaawsrlfHSRLREFEADEASAWLTgdplaLVEALRKLdyw 234
gi 517827260 170 ------------------------------lsaissrfisrtvaqifASKAREHAADRDGAKICgkpldLASALEKIern 219
gi 542111121 177 ------------------------gnplaaiamvvlapiaamlvqlaISRTREYDADEDGAKLTgdplaLASALRKLdag 232
gi 296888791 182 ----------------------kyckfigkflnilfapiasviinaiIPGSREFDADKGSSEITgdfaaLASALNKItyg 239
gi 815099558 184 ----------------------ggvgvigsiliallapfaatliqfsLSRTREFDADEDGSKLTgdplaLASALRKLeqv 241
gi 744452758 213 mlgagliyglfgswqeatgfftgviiwivlmllpsmtqflvtlltfwHSRWREYHADAVAARLTsv-daMVSALATLdrl 291
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                  #           
gi 24212639  235 vryrplrrgnpat-------------------------ahmfiinpfrgidfaELFSTHPpteKRIERLR 279
gi 122423522 249 yepqeatsmm-------------------------------afcingkskslsELFMTHPpldKRIEALR 287
gi 805374630 274 stes------------------------------------------stkglfaNLMSSHPdknDRIAALM 301
gi 150421574 238 yehnphlveearsnemasmlfivapltsltasplvdvdylverlkeqetnplvELFSTHPpvsKRLRFLD 307
gi 575416314 235 nqvtpmqvgspvm-------------------------ahlytvnpfawgkwaQGFDTHPstvDRILLLE 279
gi 517827260 220 tvsllnpvaeknp------------------------atahiyvvdplhaskrTHRSSHPateKRIARLR 265
gi 542111121 233 tqraplpqnrdlvd-----------------------vshlmianpfrgagvgKMFATHPpmaERIKRLE 279
gi 296888791 240 mqlhemkstaglqav---------------------aslmiicpwnnnapalvRLCTARPtvqDRIGRLM 288
gi 815099558 242 tahrpmeqtpanq-------------------------svaammianpfgkarNLFSTHPsmdERCARLE 286
gi 744452758 292 pfqppkkdreqms------------------------tlwvrvpmkeetgtfhWLKHTHPpihKRMEALR 337

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