Conserved Protein Domain Family
M56_like

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cd07329: M56_like 
Peptidase M56-like, integral membrane metallopeptidase in bacteria
This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain beta-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.
Statistics
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PSSM-Id: 320688
View PSSM: cd07329
Aligned: 58 rows
Threshold Bit Score: 56.3054
Threshold Setting Gi: 558527519
Created: 3-Mar-2009
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Zn binding siteputative active
Feature 1:Zn binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 558527519 117 RRKQRRRELLvrspllp--------------gedsgggPLVVLEg--------------eRPGAWWLPgaapQLVITTAA 168
gi 502886061 131 ALPENFPVLFtilrglc--------------hkadiavPRVLVTrdr-----------faRSFTFGFKk--pVIVLSQGL 183
gi 225169394 128 PSAEQSLRVDtimkkyc--------------drwqmsvPKVVFTgrk-----------gfVAFAGGLFr--pVVVVSLPL 180
gi 391353936 111 RMLSGCREVTvenapq----------------lfahldPLVRKTgiaklqvyilppergvQIFVSGIRr--pLLVISPLL 172
gi 502674466 176 ELLAMTRRLAislg---------------------irdPIEVLIlpr-----------taSPMAVGFLr--yKLVLPQPT 221
gi 493236934 170 KTVCDQLRLKqalrtgrpietpr--lsslfdetklllgVKRKVRfvas--------ehipGPAVVGFYkp-vVVISPRLL 238
gi 501196209 161 RGLRGRRAVQdarllrrle----------rlrarigmrPVALTEsag-----------vgSPLVIGAS----EICVPLAR 215
gi 648601392 183 AALAHRAGVRmp-------------------------aLYTLPDv--------------aSPFAFGRR----IVVPEWAA 219
gi 521067896 214 KDLGSRTRVDaehdanktlre------ictqidtrhvpYLSRSSdi-------------kSPICLPRR----EICLPDWA 270
gi 498270040 243 LEMLDRRELDrfvprgfrirflrlterpgfapdgavsaDLAIQNrnr----------rcsQPFACGVIh--pTVVLPQGI 310
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                       #   #                                    #                       
gi 558527519 169 Lrrl-kgRQLDAVLAHEQGHARARHDWLLHCSSalangfPQVPVFAAFQAEMHRLVELAADDVASRRfgr---ltIALAL 244
gi 502886061 184 Idnl-deDELEAVLAHELAHIIRSDSILNWITVflrdvmFFTPVMYWLFRDLSQEKEHATDDITIALsgk--plaLAGAL 260
gi 225169394 181 Leql-sdTEIEGVLLHELIHVRRGDTLSGWLFHlardlmFFSPFSTILLDRYLLERERLCDQEAVAAmgr--tktYAATL 257
gi 391353936 173 Lsal-sdSELQAVLAHELAHLVRRDQIASMVTFllrslmFYNPLLYPLVKWLKEEREKAADALATRWtkk--pfaLAGGL 249
gi 502674466 222 WwgaldrAQQMGMVAHELAHLRHRDPLWSVVGHlichtlWFQPLLWYAVKQMRMTAEFAADDEARRLsgd--glgLARSL 299
gi 493236934 239 Vtl--kkEQIQYILAHEFAHIRRWDVAMNWIMHivlilhWFNPLVWLAVHRARQDQEIACDACALDRlspqknnaYGQTI 316
gi 501196209 216 Laal-tdAEVDAVFAHELAHLERRDGLWFPAAGlvqsvlWAHPLVHWASSRFRQTAELACDDRAVELtgd--plgLARAL 292
gi 648601392 220 Tal--dpRQLQAMLAHELAHVARRDPQWRLVIEcwragfWFLPFAGVAQRRLDELAELACDAFAARQtgd--ahaVAECL 295
gi 521067896 271 Fddl-pkAELKSLLAHEMGHMVRRDPLMLMALQtlsrvfFFQPMFIVARRRLTDIAELAADEWAAKQlsd--araVATAL 347
gi 498270040 311 Edtl-ttDELRALLAHEVAHLVRRDPIWQWIVEilctclAFQPLNFLARCHWQWATELLCDDWAVDRrip--ptlLATCL 387
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
Feature 1                                                       
gi 558527519 245 VELNEdrg------------vfgPCPAPDGEl------pRRVDRLLT 273
gi 502886061 261 IKVWRmspkniwasialdnfmpyPNFAAGEGal-----eGRITRIID 302
gi 225169394 258 LKSWRivveq---------kdvsPGVAAGFVgkkq-hmeERIHSLLA 294
gi 391353936 250 VKVTKlmleqrpl--ytsyglstIELTSGGAl------rERVRLLLD 288
gi 502674466 300 ATLASwlvte--------rasdsISIATSGLssyrstlgQRVEQLLA 338
gi 493236934 317 IHVLEhfsgsp-------hqpglAALSATHKql-----kRRLTMIKQ 351
gi 501196209 293 VQVASgaslap-------rrplvPAMARSAGal-----lPRVRRLLD 327
gi 648601392 296 AACAErhfdnr-------ayalmPAMAARGSsl-----iQRIDRLLE 330
gi 521067896 348 FTCATkiqenr-------qiqwgLAMAGNKSml-----kTRVERLIG 382
gi 498270040 388 TKVAEwrldqiags-sfrgglglPAVGPDGSl------tYRMHWLLR 427

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