4AW6


Conserved Protein Domain Family
M48A_Ste24p

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cd07330: M48A_Ste24p 
Click on image for an interactive view with Cn3D
Peptidase M48 CaaX prenyl protease type 1, an integral membrane, Zn-dependent protein
This family of M48 CaaX prenyl protease 1-like family includes a number of well characterized genes such as those found in Taenia solium metacestode (TsSte24p), Arabidopsis (AtSte24), yeast Ste24p and human (Hs Ste24p) as well as several uncharacterized genes such as YhfN, some of which also containing tetratricopeptide (TPR) repeats. All members of this family contain the zinc metalloprotease motif (HEXXH), likely exposed on the cytoplasmic side. They are thought to be intimately associated with the endoplasmic reticulum (ER), regardless of whether their genes possess the conventional signal motif (KKXX) in the C-terminal. Proteins in this family proteolytically remove the C-terminal three residues of farnesylated proteins. The gene ZmpSte24, also known as FACE-1 in humans, a member of this family, is involved in the post-translational processing of prelamin A to mature lamin A, a major component of the nuclear envelope. ZmpSte24 deficiency causes an accumulation of prelamin A leading to lipodystrophy and other disease phenotypes while mutations in the protein lead to diseases of lamin processing (laminopathies), such as premature aging disease progeria and metabolic disorders. Some of these mutations map to the peptide-binding site.
Statistics
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PSSM-Id: 320689
View PSSM: cd07330
Aligned: 6 rows
Threshold Bit Score: 253.135
Threshold Setting Gi: 543112037
Created: 11-Mar-2009
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Zn binding siteputative active
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:Zn binding site [ion binding site]
Evidence:
  • Structure:Human nuclear membrane zinc metalloprotease Zmpste24p binds zinc; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
4AW6_A       118 YEITQSLVFLLLATLFSALAGLPWSLYNTFVIEEKHGFnq---qTLGFFMKDAIKKFVVTQCILLPVSSLLLYIikig-- 192
gi 223691600  80 FPTLEALVFLLVFLGYLVIVWTSAWGVQKQFFSGTVSKks---fVLSNISFSLPALLPWFLLSIMADFIQILPFegprtf 156
gi 543112037  83 YGEAGALVFVAVFAIADQLLCEVRAWARWWFAERAGGMsrsgmaTVALDSGRRVALNTVIATLAAAWVLWPFSFwafgwa 162
gi 378596780 101 NPLVAQWLVLAAVTFTAALIEFPFGWFAHFRVQERFGYml---eDRRLWMLREIRRTFAGWLVSLPVLALLIVLlehagg 177
gi 404659226 101 PGIPADWVLISLIVLLMLLIELPFGWWARYRVKERYGYmr---ePRMRWLSRTLSEALWGWLVFMPVSAALLTIfeyagg 177
gi 512682974 101 NTLLVQWSLLVSIMGLMVVVSLPLEWLIRYRLRERFGYqr---rSRKEWFKRTVGISTAGLAAALPATALLLILcevtgp 177
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
4AW6_A       193 --gdyfFIYAWLFTLVVSLVLVTIYADyiaplfdkftplpegkLKEEIEVMAKsid-----fplTKVYVVegskr--ssh 263
gi 223691600 157 latplgEILYVIFFLFAVASFGPFLIQklwgcs----sldpgpTRERIESLCNlagig--cadiLKWELFggsm----it 226
gi 543112037 163 wpisggLLIAGAALLFWARPNLIAPLFerfkp------leageLRQRLEAMMQrcgth--leqvLVVDTSrrs-----rv 229
gi 378596780 178 -awwwiGWVFFLLYLFWRWKFSNTVALfwkrrsq---pmadrkTAEMILAYMRhegfvvddvevMTKPLSwa-------h 246
gi 404659226 178 -dwwklAWGVWLVYLLWRWKLSSVYGVfwkrrsr---pyanaeTREAVRESLHrq-------giTMTEMVvmtrpaswdh 246
gi 512682974 178 -ywwllVWMLYLAWLFWRWRLSLMRGKlwsrasr---piqneaLRRKVRSLLAeegf---rmedLVIMTRppaw----ky 246
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                               #   #    
4AW6_A       264 sNAYFYgffknkRIVLFDTLLeeysvlnkdiqedsgmeprneeegnseeikakvknkkqgcknEEVLAVLGHELGHWKLg 343
gi 223691600 227 aGVMGLvs--rfRYILVTPALvsl------------------------------------mtpREVDAVIAHEIGHVKKh 268
gi 543112037 230 aNAYFTglg-rsKRVVLSDTLiaq------------------------------------lsaAELEAVMAHELGHYRC- 271
gi 378596780 247 sHLVIPgwgkkrRVVVFAHAAqrl-------------------------------------epDELLALIAHDVGHVKH- 288
gi 404659226 247 sNIVLSgwglrrRVIVFAHVAhll-------------------------------------rkDEIVALAAHEAAHVRHf 289
gi 512682974 247 aHVLLPgsgelrRVVVFADTAakl-------------------------------------teDELLAVIASQAARIKFh 289
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                      # 
4AW6_A       344 HTVKNIIISQMNSFLCFFLFavligrkelfaafgfyd-------sqptliglliifqfifsPYNEVLSFCLTVLSRRFEF 416
gi 223691600 269 HIHFYLFFFAGYIACVYALFdpllvlvyamdplswfvsf---vgmeqetfstlvfslililLFILYFRFVFGFFMRNFER 345
gi 543112037 272 GHLQRFYASQAALLFGAYLAlgavaammnlspa---------------paelvvaaylllpALGWPLQFWQSARRRRYEY 336
gi 378596780 289 LHLFLRAAVYAVTGWLVFRFagwgaehveffegfgyapavsflqdgtragyvlaiavtafpILYYPLRPAVNLFARLMQY 368
gi 404659226 290 HGVLRLLINVAVSYIFCWLAgwgathvqffegfnyspmlt-ldmpgthagsvcavalvvfpMLLYPLSPLLNLAARLMQY 368
gi 512682974 290 HGPWRIALSAAGGFITCAVLgwaantpvffeglgfspslt-vmqpgthagfamaaaviafpIVFFPLRALNNFIIRQLRY 368
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                                                   #            
4AW6_A       417 QADAFAKKLgka-kdLYSALIKLNkdnlgfpvsdWLFSMWHYSHPPllERLQALKT 471
gi 223691600 346 QADTYVFSLlgsgrsLIDTFYKIArfs----rtsPDRPNWHHFNIT--QRINFLER 395
gi 543112037 337 EADAYAAEHada-aaLISALEKLLannltaptmdRWYAALFATHPPgaSRLARLHA 391
gi 378596780 369 DADEYAAKAvst-daLVRALVKLHrdykqtltpsRLYSLFHYSRPHagMRVAHLLW 423
gi 404659226 369 DADLHAARIagl-dpMISALVKLHkdtttslspsLVYSLFHYKRPHpgMRVANLQR 423
gi 512682974 369 AADRCGAAKmgg-dtLARALAKLHrdysntltpsRFYSVLHYDHPHaaMRVAHLLK 423

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