Conserved Protein Domain Family
M48B_HtpX_like

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cd07335: M48B_HtpX_like 
Peptidase M48 subfamily B HtpX-like membrane-bound metallopeptidase
This family contains peptidase M48 subfamily B, also known as HtpX, which consists of proteins smaller than Ste24p, with homology restricted to the C-terminal half of Ste24p. HtpX, an integral membrane (IM) metallopeptidase, is widespread in bacteria and archaea, and plays a central role in protein quality control by preventing the accumulation of misfolded proteins in the membrane. Its expression is controlled by the Cpx stress response system, which senses abnormal membrane proteins. HtpX participates in the proteolytic quality control of these misfolded proteins by undergoing self-degradation and eliminating them by collaborating with FtsH, a membrane-bound and ATP-dependent protease. HtpX contains the zinc binding motif (HEXXH), has an FtsH-like topology, and is capable of introducing endoproteolytic cleavages into SecY (also an FtsH substrate). However, HtpX does not have an ATPase activity and will only act against cytoplasmic regions of a target membrane protein. Thus, HtpX and FtsH have overlapping and/or complementary functions, which are especially important at high temperature; in E. coli and Xylella fastidiosa, HtpX is heat-inducible, while in Streptococcus gordonii it is not. Mutation studies of HtpX-like M48 metalloprotease from Leptospira interrogans (LA4131) has been shown to result in altered expression of a subset of metal toxicity and stress response genes.
Statistics
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PSSM-Id: 320694
View PSSM: cd07335
Aligned: 39 rows
Threshold Bit Score: 223.228
Threshold Setting Gi: 739375120
Created: 25-Feb-2009
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Zn binding siteputative active
Feature 1:Zn binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 122423522  43 GFGGSFVSLLMSKWMALRSVGGEvie--qprNERERWLVNTVATQARQAGIA-MPQVAIYHApdINAFATGarrdaSLVA 119
gi 491085350 156 GLIGAVIGLPFSRWLAKRAHQVVvierhnesHHDYFWLYDTVARLAAKANIP-TPEVGVYQSedMNAFASGatpqhSVVA 234
gi 686529220  42 SCTLPFISLCLSRWMAKRAHQMYivdqehfqSDAEHHLFTLVETLSMKAGLGkTPQVGIYRSddINAFATGvtrnrSLVA 121
gi 244539043  44 GLGSVITTLRMSKEMVLKSVQGVpinikepkDKQERFLVETIQRQRKRLHLP-MPEILVYKAnnVNAFAAGcrknsAVIG 122
gi 730210803  46 ALTMPFFSLMFSKYYVKKAYKLTilddnytyDDDIEWYREKVYELCKRAGMKkMPEVAIYTSndKNAFATGrsknkSLIA 125
gi 685120951  66 GTIWGLIGLGRQREIAVRRHGIRl-------LADGDPLTQRVAALAAKLGLKtRPWVGVMPH--NNAYAIGsnannALVV 136
gi 19704255   56 AFGTPFISLMISKATVKRAYNIRmigdggasTEKEKLVVDTVTLLSQKLDLQkFPEIGVYPSndINAFATGasknsAMVA 135
gi 820946550  17 VVITSLFSYYLSKWLAKWAKNVKriddrrdlNPKLKEIVRIVREISRIYKMK-MPEVGVYPSqeINAFATGpa-gnTLIA 94
gi 810865000  47 SLLCAYCAFFISRYKARQSLATKfie--tprNPMEDWLLGIVAQQARQAGIR-SPKVGIYDSvdINAFASGin--rSMIA 121
gi 563110828 163 GVGLSLLGLLFGGRYIARASGDVrssfgvtsVEDGHWLAERVHGLAEKLNLP-KPAVGITNV--VNAFAMGarqksSMVV 239
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           #   #                                                        
gi 122423522 120 VSTGLLQnMSPDEAEAVIAHEISHIANGDMVTMTLIQG-------VVNTFVIFISRILAQlaagfmggnrdegeesngnp 192
gi 491085350 235 FSTGLLEqMDEAEIEAVVAHEIGHIISNDMRGMAILTG-------LISSFVLVFTLPLQGlrilnllgnsf----stive 303
gi 686529220 122 FSSAILEnMDEEALAAVAAHEIAHIANGDMLTLTLVQS-------VINSVVMLCTLPLQLlewfsrfseqg----srmmi 190
gi 244539043 123 VSTGLLKnLEESEVEAVLAHEMSHIFNGDMVTMTLIQG-------IINAYVGVIANILAQissncvsgtset-iahhrsm 194
gi 730210803 126 VSTGLLYgMSEEAVEAVIAHEVAHIVNGDMVTQTLLQSsl----rMIIGVIILPITLYRLialfvsdnen------ifvy 195
gi 685120951 137 VGQPLIDtLTEAEVDAVIGHELGHVANNDMRRMGLARS-------FQNSLVWYLGFSGTLq------------------- 190
gi 19704255  136 VSQGLLNsMNETEIIGVLAHEMSHVVNGDMLTSSILEG-------FVSAFGVIATLPFLMgennnrgr----------ra 198
gi 820946550  95 FSSNLINtMSLSEIRGVVGHELSHLIHYDIARILVVQGi-----fDVLHFVLCVLIASWLfrpseeek---------ktg 160
gi 810865000 122 VSSGMLCfMPRDETEAVLGHEISHLARHDMSRLIAYQG-------ALNLGLFYFSVLSAYgisglffnq--------tge 186
gi 563110828 240 IGKPLFA-LEKDELDAIIGHELGHILHKDIARMQFAEGfqrmlvgVVNILTVFGMFFAASasrkrangrl-----nahla 313
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                            #                                           
gi 122423522 193 liyfaVATVLELVFGILASIit-------mWFSRHREFHADAGSAKLVGrEKMIAALQRLKtsyepqeatsmm------- 258
gi 491085350 304 iilwlTKLVLSIVLTFFGSLlv-------kKYSRNREYKADAIASLLVGkDSMISALSRLSqekiaipieqrgf------ 370
gi 686529220 191 wvitlTRLLLTVILMFLGSLlv-------kAFSRHREFKADHLAAQLLDsHSMISALEYLKhqtpvtppnqkay------ 257
gi 244539043 195 vhnlcSKYLKYILFGVVSTLii-------nKFSRYREYYADAGSASIVGtEKMIAALKKLKlsyppkepdniqi------ 261
gi 730210803 196 yiilfVEIIVTTILLFISSLfi-------kSFSRKREFKADHLAAQLTTpANMILALKELDgpaqllksqkky------- 261
gi 685120951 191 ----rWARWILTWLSEFFVL----------GLSRKREYWADAIGAALTGkEHMIAALEKLHqapalsgferdh------- 249
gi 19704255  199 assmaTYYMVRNVANIFGKIvs-------sAYSRRREYGADKLAAEITDpSYMKSALLRLQeisegrislqnsdref--- 268
gi 820946550 161 elnivKFILKWIFFEILSAImrlggillvlWYNRKRELAADKRGAEIVGiDDMLAALRKLLelenrswivdgidlteden 240
gi 810865000 187 mallmMMLGAELFFSVLVVLll-------mRFSRQLEFRADAGGAALSKrAAMIAALERLNserwwpppekmav------ 253
gi 563110828 314 wgtgvVVRKTVFIGSELVTK----------GISRNREFHADAVGAHVTSvDAMARALTRLHgmgdeptaqehh------- 376
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|..
Feature 1                                      #           
gi 122423522 259 -------------afcingkskslsELFMTHPPLDKRIEALR 287
gi 491085350 371 -------------nafkisnqsqwaEIFNTHPSIEKRIQALQ 399
gi 686529220 258 -------------aafkihtssswlDILSTHPSIDRRIEALR 286
gi 244539043 262 -------------fcingkrikffpGLFSTHPSINNRIKALI 290
gi 730210803 262 -------------aaiqfngmgrflDIFSTHPSINRRIKYIE 290
gi 685120951 250 --------------arlmfrgfakgSLWSTHPTLEQRRAALQ 277
gi 19704255  269 ---------asfkitnnfsmgnifgNLFASHPSLAKRIAAIE 301
gi 820946550 241 tkatgepnsvsllkfnpekkkrgllELFRTHPRLEERIERLE 282
gi 810865000 254 -------------cgikgsissglrHLLMSHSTLGERISLLK 282
gi 563110828 377 -------------ygylmfrgtgfgNLFSTHPTLQARLKALN 405

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