Conserved Protein Domain Family
M56_BlaR1_MecR1_like

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cd07341: M56_BlaR1_MecR1_like 
Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase
This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain ?-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.
Statistics
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PSSM-Id: 320700
View PSSM: cd07341
Aligned: 234 rows
Threshold Bit Score: 86.2296
Threshold Setting Gi: 748177613
Created: 11-Mar-2009
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Zn binding siteputative active
Feature 1:Zn binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 493236934 159 AWLSGVLIlt----vKTVCDQLRLKQAlrtgrpietprlsslfdetklllgVKRKVRFVase----------hipGPAVV 224
gi 495900747 108 MYWVGVAV-------SLAVRLWQFGRMrwfi----------------prgcLWKECREDgitl--------ychaRPVAP 156
gi 312941258 102 VIVLGISLisilfliKLIYSHFQIIRLkkqs-----------------eeiIENGYRIYcg-------------eFESPF 151
gi 763343488  97 IYFLVFFIll----tRFIISNFKLLKF------------------------IRFHQRVNfkgstl----iytdgkYPTFA 144
gi 522022285 118 IYLLGTLLas----iRFFRGIIKLIKL------------------------IRTSPRESrrg----------ftwVKMPV 159
gi 365755492  77 LLVVSLVLf-----vRLLIGIYKIYQMk----------------------nQYQGKELKgvrfy-------itdlHDAPF 122
gi 507725608  76 LYWIGVAI-------FSINFIVQLITL------------------------IYRSYSRPviqdgrfrivelsgeqAPCSF 124
gi 763312800  83 VPLEGILFf-----sVLGGSLLIFGQLcyk------------------fwlLFNIAKYSfpdtwr----gygfrnTPKTI 135
gi 563394906 100 AWLLGIGV-------LLIRFGIRLRSLlclicg-------------aeraeIEGVPVYF----------------APRVR 143
gi 570747023  92 TWDWVLLI-------AALGSLILLARFlimfc---------------sflrIKRKAQLIsddrtri--yqldedmRPFSF 147
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                     #   #                                       #      
gi 493236934 225 GFYkp--vVVISPRLLVtlkkEQIQYILAHEFAHIRRW---DVAMNWIMHIvliLHWFNPLVWLAVHRARQDQEIACDAC 299
gi 495900747 157 FSWm---cCVVMSEEDLe--gAAGEAIFAHEKAHVLHRhsyDTMLMLAAEA---IQWFNPVIWMMEMDMRCIHEYQADEY 228
gi 312941258 152 SYFn----AVFVPNDLLs--ePSFDTVLEHELVHLRKKhsvDRVLMEIILS---IFWFNPLLYLFRNRLIEIHEFQADAE 222
gi 763343488 145 YLK-----YIFWDNTTKlt-tKEQEMIFQHERTHIKELhslDIFLMEILKI---IFWFHPAIYLIDAALRTQHEYLADQK 215
gi 522022285 160 GFQpssffLWVLIDEEK----LIDREILKHEMTHVKQWhslDILIFELATI---LLWFNPVIYLFKRDLRQIHEYLADAN 232
gi 365755492 123 SFFk----NLFWKKSIEid-sDLGKQILKHEMVHIEQKhswDKIFIEFVQA---VFWFNPIFYWIKKELFLIHEYLADKK 194
gi 507725608 125 GNT-----IFINPEKYDw---DTYSQIILHEKVHIQQGhsyDIIFAELALV---FQWFNPFAWLYRKAVEDNLEFLTDNE 193
gi 763312800 136 QPFsf-wkTIYLNISQHk--sTELSDIFRHEQAHIDGLhsfDMILAECCCI---VLWINPFSWLMKKAIVENLEFLTDNR 209
gi 563394906 144 MPFsf-cgRIYLPTDLRs--yPSVRNVLIHEQEHTRAThfvDLLIAEVACM---LFWFNPFAWALKRDMQRNLEFLADRA 217
gi 570747023 148 GNA-----IFINTELHSg---EELEEIIRHEFVHVKQKhtlDIIWSELLCI---LLWFNPFVWLLRKSIKQNLEFLADKQ 216
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                                                                
gi 493236934 300 ALDRLSPqknnaYGQTIIHVLEHFsgsphqpgLAALSATHKql--kRRLTMIKQFQ 353
gi 495900747 229 VLNQGINak--nYQLYLIKKAVGSrlq---sfANGLNQSTLk----KRIAMMCNRK 275
gi 312941258 223 VIALKKNpi--gYQEILFQQIKTQk-------AIASANYFKlntikTRIKMINKNP 269
gi 763343488 216 ANQMTNDa---sYSQLMVQSLFEDi-------SLNVGHGFQfstvkSRIKMLKKER 261
gi 522022285 233 AMGEKPKf----YAQKMLTLATGNk-------IVLLTNSFAflplkHRIKMLTRIK 277
gi 365755492 195 AVKQNDTk---aFAQMLLASKFSGthl---paTSPFLSSNLk----KRLQMLTKPH 240
gi 507725608 194 LLEHKEVdp-gsYQLSLVKVSAPHf-------PVSLTTNYNqsvlkKRLIMMNAKK 241
gi 763312800 210 VLEAGVNrk--aYQFSLLNVAMQGk-------PVNVTNQFNyrilkRRIIMMNKQK 256
gi 563394906 218 VLLSGVSrr--eYQYELLGFTLEVa-------AGPLCSSYNindlkERIRMMNKTK 264
gi 570747023 217 VLQNGMDkk--eYQYLLLKVMGNK--------QFAFTNHFNfsslkNRIAMMNTLR 262

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