2FBL,3BHD,2FJT,1D8H,3G3T,1D8H,2GFG,1YEM,3G3U,3G3R,3BHD


Conserved Protein Domain Family
CYTH-like_Pase

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cd07374: CYTH-like_Pase 
Click on image for an interactive view with Cn3D
CYTH-like (also known as triphosphate tunnel metalloenzyme (TTM)-like) Phosphatases
CYTH-like superfamily enzymes hydrolyze triphosphate-containing substrates and require metal cations as cofactors. They have a unique active site located at the center of an eight-stranded antiparallel beta barrel tunnel (the triphosphate tunnel). The name CYTH originated from the gene designation for bacterial class IV adenylyl cyclases (CyaB), and from thiamine triphosphatase. Class IV adenylate cyclases catalyze the conversion of ATP to 3',5'-cyclic AMP (cAMP) and PPi. Thiamine triphosphatase is a soluble cytosolic enzyme which converts thiamine triphosphate to thiamine diphosphate. This domain superfamily also contains RNA triphosphatases, membrane-associated polyphosphate polymerases, tripolyphosphatases, nucleoside triphosphatases, nucleoside tetraphosphatases and other proteins with unknown functions.
Statistics
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PSSM-Id: 143620
View PSSM: cd07374
Aligned: 38 rows
Threshold Bit Score: 62.0855
Threshold Setting Gi: 85544657
Created: 4-Oct-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site
Evidence:
  • Comment:This active site includes the putative metal binding and triphosphate binding sites.
  • Structure:3BHD_A; Human ThTPase bound to a citric acid molecule and a sulfate ion, contacts determined at 4A.
    View structure with Cn3D
  • Comment:For 3BHD_A, the citrate is positioned similarly to the triphosphate of ThTP, the sulfate ion is positioned similarly to the alpha-phosphate of the substrate.
  • Structure:1D8H_A; Saccharomyces cerevisiae mRNA-RTPase monomer bound with Mn2+; contacts determined at 4.5A.
    View structure with Cn3D
  • Structure:3G3R_A; Saccharomyces cerevisiae PolyP Polymerase bound with Mn2+; contacts determined at 4.5A.
    View structure with Cn3D
  • Structure:3G3R_A; Saccharomyces cerevisiae VTC4 polyP polymerase bound with a substrate analog (adenosine-5prime-[(beta,gamma)-imido] triphosphate) and Mn2+, contacts determined at 4.5A.
    View structure with Cn3D
  • Comment:Some of these residues were implicated from mutation analysis of Clostridium thermocellum TTM.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        # # #                                        #                      # # #       
2FBL_A         5 EIERKFLvatfpd---------------------gelhaVPLRQGYLTtpt------------dsIELRLRQQgt----- 46
3BHD_A        26 EVERKFLpgpgteerlq-------------elggtleyrVTFRDTYYDtpels-------lxqadHWLRRREDs------ 79
3G3T_A        11 RQTTKYWvhpdnitelkliilkhlpvlvfntnkeferedSAITSIYFDnenldlyygrlrkdegaEAHRLRWYggms--- 87
3G3U_A        11 RQTTKYWvhpdnitelkliilkhlpvlvfntnkeferedSAITSIYFDnenldlyygrlrkdegaEAHRLRWYggms--- 87
3BHD_B        26 EVERKFLpgpgteerlq-------------elggtleyrVTFRDTYYDtpels-------lxqadHWLRRREDs------ 79
gi 195639840   2 EVEIKLRlpdaaahrrls-----------aflaprlrrtHAQRNLFFDdaart-------lgaatAALRVRLYdgpddr- 62
gi 147796316   2 EVEVKLRlpdaashqkls-----------dllapfhvktLIQENIFFDgaaae-------lsskfAVLRLRFYdld---- 59
gi 168052253   2 EVEVKIRlddalahqkva-----------allasnhqvtHLQENVFFDgaqqe-------lsskrAVLRLRFYngd---- 59
gi 75206194    2 EVEVKLRlltaaahlrlt-----------tlltpyhlktLHQRNTFFDtpknd-------lslrrAVLRLRFLqnaavsa 63
gi 159479598   2 EVEIKIRlpdraayeqvaaa------laaapggkgrldsHAQANYFFDgpnqe-------lnsrrVVLRVRTYdvd---- 64
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    #               #                                                   
2FBL_A        47 ------eyFMTLKSEgg-------lsRQEYEiqidvtqf----------------------------------------- 72
3BHD_A        80 --------GWELKCPgaagvlgphteYKELTaeptivaqlckvlradg-----------------------lgagdvaav 128
3G3T_A        88 ----tdtiFVERKTHredw---tgekSVKARfalkerhvndflkgkytvdqvfakmrkegkkpmneienlealaseiqyv 160
3G3U_A        88 ----tdtiFVERKTHredw---tgekSVKARfalkerhvndflkgkytvdqvfakmrkegkkpmneienlealaseiqyv 160
3BHD_B        80 --------GWELKCPgaagvlgphteYKELTaeptivaqlckvlradg-----------------------lgagdvaav 128
gi 195639840  63 ---apsraVLALKRRariea--gvsrVEEIEeplepalavactddparlggld-------------spiirlvaaeygvg 124
gi 147796316  60 -----srcVLSLKSQpqisn--gisrAKEVEeeiepligracvaepwrfksm---------------ssriikrlrdefe 117
gi 168052253  60 -----qrcVVTLKGKaviid--gisrGSEVEedidvalaracvadpsqfig-----------------sdckliqqiire 115
gi 75206194   64 aspspprcIVSLKAKptlan--gisrVEEDEeeieywigkecvespaklsdig--------------srvlkrvkeeygf 127
gi 159479598  65 -----kkaTVTLKGKqilen--gigrASEVEaevppaaaaayltqpsrmla------------------evpivkdaaek 119
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     # #                     #                                          
2FBL_A        73 emlwpateGRRVEKTRYSGKlp--------dgQLFELDVFAgh------------------------------------l 108
3BHD_A       129 lgplglqeVASFVTKRSAWKlvllgadeeepqLRVDLDTADf-------------------------------------- 170
3G3T_A       161 mlkkklrpVVRSFYNRTAFQlpg------darVRISLDTELtmvrednfdgvdrthknwrrtdigvdwpfkqlddkdicr 234
3G3U_A       161 mlkkklrpVVRSFYNRTAFQlpg------darVRISLDTELtmvrednfdgvdrthknwrrtdigvdwpfkqlddkdicr 234
3BHD_B       129 lgplglqeVASFVTKRSAWKlvllgadeeepqLRVDLDTADf-------------------------------------- 170
gi 195639840 125 gdaapflcLGGFGNTRAVYEyeledg-gggvvLELDETRFDf-------------------------------------- 165
gi 147796316 118 vgdeglvcLGGFRNVRAVYAwng-------lnLELDETLFDf-------------------------------------- 152
gi 168052253 116 ykceiflcLGGFRNVRNVYDweg-------vkLELDETHYAf-------------------------------------- 150
gi 75206194  128 ndflgfvcLGGFENVRNVYEwrg-------vkLEVDETKYDf-------------------------------------- 162
gi 159479598 120 fgvgslvaLGGFQNQRDVYEweg-------htLELDETKFEh-------------------------------------- 154
                        250       260       270
                 ....*....|....*....|....*....|
Feature 1              # #                     
2FBL_A       109 sPLMLVEVEFlse---------daAQAFIP 129
3BHD_A       171 -GYAVGEVEAlvheeaevptalekIHRLSS 199
3G3T_A       235 fPYAVLEVKLqtqlgq---eppewVRELVG 261
3G3U_A       235 fPYAVLEVKLqtqlgq---eppewVRELVG 261
3BHD_B       171 -GYAVGEVEAlvheeaevptalekIHRLSS 199
gi 195639840 166 gTRYELECETaepdr----vkavlERLLTV 191
gi 147796316 153 gTNYEIECESseper----akkllEGFLEE 178
gi 168052253 151 gTTYEIECETndper----frqllGELLDK 176
gi 75206194  163 gNCYEIECETeeper----vktmiEEFLTE 188
gi 159479598 155 gTLYEIEVETeqpev----lrdrlEHWLSG 180

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