1UG9,1LF6,1LF6


Conserved Protein Domain Family
GH15_N

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cd07430: GH15_N 
Click on image for an interactive view with Cn3D
Glycoside hydrolase family 15, N-terminal domain
Members of this family are N-terminal domains uniquely found in bacterial and archaeal glucoamylases and glucodextranases. Glucoamylase (glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; amyloglucosidase; exo-1,4-alpha-glucosidase; gamma-amylase; lysosomal alpha-glucosidase; EC 3.2.1.3) hydrolyzes beta-1,4-glucosidic linkages of starch, glycogen and malto-oligosaccharides, releasing beta-D-glucose from the non-reducing end. Glucodextranase (glucan 1,6-alpha-glucosidase; exo-1,6-alpha-glucosidase; EC 3.2.1.70) uses an inverting reaction mechanism to hydrolyze alpha-1,6-glucosidic linkages of dextran and related oligosaccharides, releasing beta-D-glucose from the non-reducing end. These N-terminal domains adopt a structure consisting of antiparallel beta-strands, divided into two beta-sheets, with one sheet wrapped by an extended polypeptide, which appears to stabilize the domain. The function of these domains in the enzymes is as yet unknown. However, it is suggested that domain N of bacterial GA is involved in folding and/or the thermostability of the A domain that forms an (alpha/alpha)6-barrel structure.
Statistics
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PSSM-Id: 143632
View PSSM: cd07430
Aligned: 42 rows
Threshold Bit Score: 227.488
Threshold Setting Gi: 222479924
Created: 4-Oct-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Domain
Conserved site includes 29 residues -Click on image for an interactive view with Cn3D
Feature 1:Domain interface
Evidence:
  • Structure:1UG9: Arthrobacter globiformis glucodextranase interface between N domain and A domain, including linker region; contacts 4.0A
    View structure with Cn3D
  • Structure:1LFG: Thermoanaerobacterium thermosaccharolyticum glucoamylase interface between N-terminal domain (all beta-strand) and C-terminal domain (all alpha-helical), including the linker region; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  ######## ## ##        ## # #        # #   ##                           
1UG9_A          6 PGSPGa--aATWTKGDKeGVGTSln----paSKVWYTLTEGtm-sEVYYPhADTPNTRELQFAVSd---gTSAQRese-- 73
gi 55378685   694 TEGPSe--rPTWTTGEKyGIGTVadhdeqdpSRVWFTLTEGal-tEARYPqVDMMNLRTLDFLVRctdetDYAVRthren 770
gi 81654707    30 FGSPGg--aPVRGPAAKsFVGTAvn----spSRVYFTGYRGiv-sEVYYPlLDTPESVDLQFLVGda-grTFVDEekr-- 99
gi 115378229   44 PGHPGa--pALWTPANKaGFGTArs----aaSRVWYTLGSRgdltEVYYPtLSTPSVRELRFVVSdg--rTFAELese-- 113
gi 134102743   34 PGAPGt--dPGYAGADKlGFGTSrd----aqSPVWFTLGRGga-aDLYYPnLSTPASRQLQVVVTdg--aGFVQRlsd-- 102
gi 162452301   33 AKGGGv--pSVWGPAEKsFLGTStt----atSRVYFTGHRGiv-sEVFYPvLDTVNTVDLQFLVGdt-akTFVDEekq-- 102
gi 167588842   15 AGSPSaqarGVWNPANKhGFGTStt----leSKVWFTLGSTgl-dEIYYPrLDTPSVRDLRFVVSdg--eTFAERvtd-- 85
gi 182680331   27 PGAPGy--kQGWSPANKqGFGTStt----heSNVWFTLGGSgl-tDVYFPrIDTPSIRDLQFVVSdg--rTFAERese-- 95
gi 222479924  727 PRGPDe--aPHWTTGRKfGVGTPadhkaadpVPVWFTLTEGal-tEARFPrIDVMNVRTFDFLVAdp-etGYTVRtfdet 802
gi 227387549  528 IEGPTe--rPTWTTGEKfGLLTAadhyeddpSRIWCTLTRGav-tEVRFPrVDLMNLRTLDFLIRss-dgEYTVRthret 603
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   # #         # #                                                       
1UG9_A         74 -----qttRTVELAdp----kaLSYRQTTTdn----agRWRLTKTYVTDPrrSTVMLGVTFEVldg---gdYQLFVLSDP 137
gi 55378685   771 rrtddsvrRRVEPVdd----eaLLYRHVFTetgdgqghEWTLTVDYATDPehDAIVADVSFSSadd---ksYDVFSVADI 843
gi 81654707   100 ------qaYSASQSdk----rtMSWVASTGna----ghNWQISKRIFADPnrDALVQRVTFTAlnghtvgdFNLYALFKP 165
gi 115378229  114 -----ateHRVELAdp----rsLTFRQVNTar----sgRYRLTKTYVTDPerHVVLIGVRFESlsg---epYSLHVVYDP 177
gi 134102743  103 ------vpTRTEPVdp----ktPSYRQVSTg------qGWEATATYVTDParPSVVVDLQVRSldg---kpLQTYVLHEP 163
gi 162452301  103 ------qsFTVTQPdk----rsMRWQATTTns----ahNWRITKTVFTDPgrHALIQRVRFDAlggrnvtqFNLYLLHNP 168
gi 167588842   86 -----hatHETTLIda----dgLTYRQVDVdn----agRYRLTKTYVTDParPVVLVDVTFESldg---rpYQVYALLNP 149
gi 182680331   96 -----atrYHVELAap----hsLVYRQINTan----nkRYRLTKTYSTDPerAVLLVDVTFESldg---hpYQLYALLNP 159
gi 222479924  803 s--hvtatETVERTteptvddaLAYTQTICetgdghghSWTLTVEYAVDTdgDAILADVAFEGsr-----eYDVYALVDT 875
gi 227387549  604 rraddsveRRVEPLdd----esLRFRHVFVetgdgrghEWELTVEYAVDPahDALLADVDFEAadg---edYDLFAVADT 676
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #                      #                                                        
1UG9_A        138 Sla------------------gtSGGDTGSVTd----gALLASdlad---------------aatpVATALVssv----- 175
gi 55378685   844 AltstitedrairygqpgsyhlvGRNPRAYTDqtd-ndLLVDEngd-----------------aysIAVAMAaag----- 900
gi 81654707   166 Yld------------------nsGSGNSAQTVavaggyALAASrn--------------------sRASALMaslpwktv 207
gi 115378229  178 Sls------------------ndGMDDVGTSQe----gALLASdg--------------------tSASALLaap----- 210
gi 134102743  164 Tlt------------------kdGSDDRSRTEg----aTLVASdr--------------------sAASALRatp----- 196
gi 162452301  169 Amd------------------nsGAGDTSRTVdaggrtLLVASen--------------------sRASALAasrpwkvv 210
gi 167588842  150 Apg------------------naLTNTTGETNg----aTLTARng--------------------nVATAFAssp----- 182
gi 182680331  160 Apg------------------ndQMHTHGETFh----hALVASng--------------------nVASAFTadp----- 192
gi 222479924  876 Tit------------------nvGSNDRGDRVddsdgyHLLARnndaaerstgk--lvdddgdsfeVALAIDsdd----- 930
gi 227387549  677 Slt------------------ntGTAERGLRLgepgahHLVARdptaytgetddpllvdeegeaysVAMAMTatt----- 733
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                #                                        
1UG9_A        176 -gfgAVANGYvgtSDGWTDLaadgr---ldnaSATAGpGNISQTGQIPlaag--gkteFSLALGFGad--taEALATAKA 247
gi 55378685   901 -rfdWATVGAa-gSDRLDGLysdgd---lpdpVESIDgTNLVLIGRLDsgt----rvrDTVALGFArqadtaAALGEADG 971
gi 81654707   208 ngqaMLSSGFvgqSDGWTDLigsgdn-tmnwtYGSATnGNVAQTGWLDlgdptatsvsFDMVLAFGkn--qgDAVNAAAA 284
gi 115378229  211 -altRTSSGYlgvSDGWSDLqdnfq---qdwaYASAApGNVVQTAQLEvdgr--drqqVTLALGFGtt--pdEARVAARD 282
gi 134102743  197 -aftETSSGYlgaSDGWTDLsrhkr---ltahHPEAGaGHVGQVGRLPvdgv--gtshAVLTLGYGkh--asEAVATAEE 268
gi 162452301  211 sgapMVSSGFvgeSDGWTDLlggnvdrtmtwrNDLASgGNVAQMGWIDlgasavtsvsFDVVLGFGat--eiEAAATANA 288
gi 167588842  183 -pftRLSNGHagaSDGWQDIgrhfr---mtwtYACAPrGNVVQTGHTPldgv--trthVTLAIGFGad--taQAASAAQC 254
gi 182680331  193 -pftRTSNGYlgvSDGWQDLsshhh---mewtYVVADqGNVVQTGQTTldgv--trkhLTIAIGFGst--ttQAASQAQD 264
gi 222479924  931 -gfeWASVLAa-gSDKAGALfadgd---rgegTETAT-GNVVLAGLVGsgt----evsDAVALGFAenadtaAALGEARG 1000
gi 227387549  734 -rfdWATVAAa-gSDHLKDLfadge---lpppKDDVDnENIVLLGRIGsge----rtvETLALGFAryadtaAALGEATG 804
                         330       340
                  ....*....|....*....|
Feature 1                         #  #
1UG9_A        248 SLgtgykkVSKSYTGEWKKY 267
gi 55378685   972 ALdrgyetVSAEYADSWGDF 991
gi 81654707   285 ALgsdlsaAQQQYDNAWHAY 304
gi 115378229  283 SLasgfeqVAERYAAGWHGY 302
gi 134102743  269 TErtgfeaAARAYGEGWHRY 288
gi 162452301  289 TLgsnlstLQTTYDNGWKSY 308
gi 167588842  255 TLadgfasTARLYAEGWHRY 274
gi 182680331  265 ALrtgfdrIAQAYAYGWRRY 284
gi 222479924 1001 AIsrgfadISESYVETWREW 1020
gi 227387549  805 ALdrgygmAEAAYDDTWAAF 824

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