1D8H,1D8H,2QY2


Conserved Protein Domain Family
CYTH-like_mRNA_RTPase

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cd07470: CYTH-like_mRNA_RTPase 
Click on image for an interactive view with Cn3D
CYTH-like mRNA triphosphatase (RTPase) component of the mRNA capping apparatus
This subgroup includes fungal and protozoal RTPases. RTPase catalyzes the first step in the mRNA cap formation process, the removal of the gamma-phosphate of triphosphate terminated pre-mRNA. This activity is metal-dependent. The 5'-end of the resulting mRNA diphosphate is subsequently capped with GMP by RNA guanylytransferase, and then further modified by one or more methyltransferases. The mRNA cap-forming activity is an essential step in mRNA processing. The RTPases are not conserved among eukarya. The structure and mechanism of this fungal RTPase domain group is different from that of higher eukaryotes. This subgroup belongs to the CYTH/triphosphate tunnel metalloenzyme (TTM)-like superfamily, whose enzymes have a unique active site located within an eight-stranded beta barrel. The RTPase domain of the mimivirus RTPase-GTase fusion mRNA capping enzyme also belongs to this subgroup.
Statistics
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PSSM-Id: 143621
View PSSM: cd07470
Aligned: 28 rows
Threshold Bit Score: 77.0631
Threshold Setting Gi: 171848877
Created: 1-Oct-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:This active site includes the metal-binding and gamma-phosphate binding sites.
  • Comment:Based on the Saccharomyces cerevisiae mRNA-RTPase structure and mutational studies.
  • Structure:1D8H_A; Saccharomyces cerevisiae mRNA-RTPase monomer bound with sulfate and manganese ions, contacts determined at 4.5A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                        # #                                             
1D8H_A        45 KSVQDWVYATIYSiape----------lrsfIELEMKFGviidakgpdrvnppvss--------qcvfteldahltpnid 106
2QY2_A        22 NEIVEMLRDYSNGd----------------nLEFEVSFKni--------------------------------------n 47
gi 71667787   26 KALFDAVNKHLSNp----------------fIEVEMRLGqfkveedalrentsdqng-----aqtnglrivdanftacvs 84
gi 66805201   53 NRIIELSKQHGGId----------------sIEVEGRIGlfsntsn----------------------------gntfkp 88
gi 72386777   34 RTIVEELFQRLQSldh-------------thMELEARLCrraevsdksgstsserew------kgrrarktrtevmpgva 94
gi 224000473  52 TQPEEAIKMFLIQarstarrrppqyggspppIEIEARLGtilspfgahdmral--------------ssgaklipikgvn 117
gi 154338896  69 LEVAESLLARLKPflr------------eshIEIEARICsiiqskkrthsmmkghv--------gatattissrdaphiv 128
gi 156086038  35 GPLAAALDNGDFDstsgy---------ppceLEIEVRLGsirnvrdgqrftlpsat-------dalisdrafvrfqpsvt 98
gi 71032261   50 GPLFEALTNNHFIksgynmnsgsvfskpecqLEVEVRLGfiqnirsehrftlpvtt-------dtllasrapvkfvpgvt 122
gi 209875341  21 ITEVESIAQALVDlvegfpei--ledncnidLEIEGRIGtimsdtsreriklpcegltllapsdkdfpyrfvpgvqkwqm 98
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              #                                         
1D8H_A       107 ASLFKELSKYIRGIsevte------ntgkfSIIESQTRDSVYRVgls--------------------------------- 147
2QY2_A        48 YPNFMRITEHYINItp------------enKIESNNYLDISLIFp----------------------------------- 80
gi 71667787   85 TEDYERIKTYLMTE----------------MENSSMTRSVTHDVs----------------------------------- 113
gi 66805201   89 GMVRDDWNDLYHHLk--------------lKSEPVATTETDYIY------------------------------------ 118
gi 72386777   95 EEDYKRIEEFCLDK----------------GKEGSVTRSTTRDVs----------------------------------- 123
gi 224000473 118 RVVHAFVCNISGEGnsnant----tsaaktNFEGGITRSNYLKWtnaglselgpltnafsikhknpnesesaaikfqlae 193
gi 154338896 129 EGEHRRIQVGVSAEdfarmkaymaekpealPFQESVTEDVNTRAg----------------------------------- 173
gi 156086038  99 KSQFQSAFTLIKQIaeergt-----sdewdTQLGILTFDRFFKLpe---------------------------------- 139
gi 71032261  123 EDQYNAARNFLIETseeddp-----knewtTCLNSCTINRFFEIqgy--------------------------------- 164
gi 209875341  99 QAVIDELKRNLEETki-------------pYKVNTRNTSDYYVLnsens------------------------------- 134
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                      #                                     #   #                       
1D8H_A       148 -------tqrPRFLRMSTDIktg--------------------------rvgQFIEKRHV--AQLLLYSpkd-------- 184
2QY2_A        81 ---------dKNVYRVSLFNqeqigefitkfskassndisryivsldpsddiEIVYKNRGsgKLIGIDNw---------- 141
gi 71667787  114 ----------LRGWRHTYATdedgn----------------------ptrcvSIVRKKRL--FVKNIVVplg-------- 151
gi 66805201  119 ----------SDSIRVAYDEhs-----------------------------kKCLRKDKKt-DKTSFDQstnl------- 151
gi 72386777  124 ----------FGQWRYTYTSgkps-------------------------eciACIRKERL--FVLDVPVpsg-------- 158
gi 224000473 194 vesvetvfcyPNSTRINFPHnppqsn---------------------vltkgHQEKKEKL--SVIDIALpaa-------- 242
gi 154338896 174 ----------RYTYAIANDGses---------------------------fiGCIEKRRL--CNVEVRVpgc-------- 206
gi 156086038 140 ---------yEEPVRISVPQnqtkg-----------------------aksfEAIRKVKL--LTWNVSTgssmrdtqmnd 185
gi 71032261  165 --------deLVRISVSNDSnspvnr--------------------qqkepvDAIRKVNL--LTWNISSgsteepseese 214
gi 209875341 135 ------shsdKSLIRVSFISpktat-----------------------dkpvDAIWKEKI--AIFDFYSpgynndidgh- 182
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           #                       # # #              #                 
1D8H_A       185 ---------sydVKISLNLELPvpdndppe---kyksqspISERTKDRVSYIHnd--scTRIDITKVENhnqns------ 244
2QY2_A       142 -----------aITIKSTEEIPlvagkskiskpkitgserIMYRYKTRYSFTInk---nSRIDITDVKSspiiw------ 201
gi 71667787  152 ---------aynLRFAVSTETPgdlrfs------gagpraGHTRLKDRLSITDg----mFRYDMTQVTEk---------- 202
gi 66805201  152 ---------iydFRISTSIEEKfpppls-------lppgyIIRREKQRYTFTEd----qWKIDLTKVIVrpdfn------ 205
gi 72386777  159 ---------aydIRFSACTEITgelpsp------nfaptrGYTRHKDRLSVTDg----lFRYDLTRVRDk---------- 209
gi 224000473 243 ---------pydLRLQLATEVPmgegnnipn-vksiphgwNKKRVKRRRSYSRldnsfaWKIDVTEVTStdapaaggasa 312
gi 154338896 207 ---------pydIRVSVSTEVPkpaida------pavkphGCVRRKRRWTATEk----tFEYAFTRVSTvd--------- 258
gi 156086038 186 dnddvgdqeildYRIAVNLEYRvpirdl------pntsaaTTRRKKNRNTYINkk--ghLRFDLTQVQTvesdss----- 252
gi 71032261  215 --efstglegldYRIAVNLEYKvpltnl------pttsnaKYCRRRLRDSFIHte--skMRFDLSKVEDvten------- 277
gi 209875341 183 --cnytdqtfkdFRIAINIEHKenptlimnslvgqienvcVLERRRNRETLDVs----tFILDATNVTQiesrr------ 250
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*....
Feature 1                        # # #                                      
1D8H_A       245 -------ksrqsETTHEVELEINtpallnafdni----tndskeyaslIRTFLNNGTII 292
2QY2_A       202 -------klmtvPSNYELELELInk---------------------idINTLESELLNV 232
gi 71667787  203 -----------gVLMHEVEIEGVfsshek-------------qlteswLEELLRRAMRL 237
gi 66805201  206 --------atveQELYEVEIELFpeaiqacq---------ektsltelLNDFLNAIKGL 247
gi 72386777  210 -----------hTTGYEVEVEFLlkdndek------------kitdshVEELVGRALSL 245
gi 224000473 313 aaasmkgsgqhaDIGYEVEMELSssmt------------------qklIQTQDEAAARK 353
gi 154338896 259 ----------hkYPVFEVEVEGIhascqa-------------gvtkawLADLLGRLVAL 294
gi 156086038 253 -------vtddnNAQYEVEAELNggviinllkstglsydkkiqllkhhCSTLIRSVRRL 304
gi 71032261  278 --------kdptTSKFEIEIELLgeeivkslmdkslssdqrmmnlkyhCSTLIRSSRYI 328
gi 209875341 251 --------gyrhNETYELELELKsshflp-------------llksyvKELNNGNLKII 288

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